LOCUS Exported 2413 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION part designed to occupy position 21 of EMMA. Functional category: Selection marker. ACCESSION . VERSION . KEYWORDS YCe2040 HC_Kan_PuroR_p21 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2413) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 2413) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2413 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 71..667 /codon_start=1 /gene="pac from Streptomyces" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA PRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA" primer_bind 564..584 /label=Puro-F /note="Puromycin resistance gene, forward primer" terminator 756..785 /label=T3Te terminator /note="phage T3 early transcription terminator" rep_origin 807..1394 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1295..1314 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1406..1433 /label=T7Te terminator /note="phage T7 early transcription terminator" CDS complement(1457..2272) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 2180..2199 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" promoter complement(2273..2375) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" terminator 2376..2407 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" ORIGIN 1 tatggaggtc aggtatgatt taaatggtca gtattgagcg atatctagag aattcgtcac 61 gtctcgtgga accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag 121 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga 181 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg 241 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac 301 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt 361 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc 421 caaggagccc gcgtggttcc tggccaccgt cggcgtgtcg cccgaccacc agggcaaggg 481 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc 541 cttcctggaa acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt 601 caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg 661 tgcctaataa gttgcgagac gaaaacgaat tcaagcttga tatcattcag gacgagcctc 721 agactccagc gtaactggac tgcaatcaac tcactggctc accttcacgg gtgggccttt 781 cttcggtaga aaatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 901 actctttttc cgaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 961 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 1021 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 1081 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 1141 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 1201 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 1261 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 1321 ctgtcgggtt tcgccacctc tgacttgagc atcgattttt gtgatgctcg tcaggggggc 1381 ggagcctatg gaaaaacgcc agcaacgcag aaaggcccac ccgaaggtga gccaggtgat 1441 tacatttggg ccctcattag aaaaactcat cgagcatcaa gtgaaactgc aatttattca 1501 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact 1561 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc 1621 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat 1681 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga 1741 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcacca accaaaccgt 1801 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgccgtta aaaggacaat 1861 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt 1921 cacctgaatc aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg 1981 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa 2041 attccgtcag ccagtttagc ctgaccatct catctgtaac atcattggca acgctacctt 2101 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg 2161 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt 2221 tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc attttagctt 2281 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat 2341 tatggtgaaa gttggaacct cttacgtgcc gatcaagtca aaagcctccg gtcggaggct 2401 tttgactttc tgc //