LOCUS       Exported               13259 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  For red/far-red light-regulated gene expression in Saccharomyces 
            cerevisiae. Integrates into the yeast ura3-52 locus. Encodes 
            constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD 
            fusions..
ACCESSION   .
VERSION     .
KEYWORDS    pRL_CT_GI_del1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13259)
  AUTHORS   Hochrein, Lena , Fabian Machens, Katrin Messerschmidt, Bernd 
            Mueller-Roeber
  TITLE     PhiReX: a programmable and red light-regulated protein expression 
            switch for yeast.
  JOURNAL   Nucleic Acids Research,
REFERENCE   2  (bases 1 to 13259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13259
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             192..1286
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        1416..2082
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase"
     primer_bind     1952..1975
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             complement(7650..7799)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(7830..7895)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(7902..7922)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(10487..10506)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             11128..11988
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11346..11365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12159..12747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12648..12667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12901..12918
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat cacaatactt
       61 gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt agcaatcgtc
      121 ttactttcta acttttctta ccttttacat ttcagcaata tatatatata tttcaaggat
      181 ataccattct aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc
      241 aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg
      301 tcaagttcga tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgttc
      361 cacttccaga tgaggcgctg gaagcctcca agaaggctga tgccgttttg ttaggtgctg
      421 tgggtggtcc taaatggggt acaggtagtg ttagacctga acaaggttta ctaaaaatcc
      481 gtaaagaact tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt
      541 tagacttatc tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag
      601 aattagtggg aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt
      661 gggatagtga acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca
      721 tggccctaca acatgagcca ccattgccta tttggtcctt ggataaagct aatgttttgg
      781 cctcttcaag attatggaga aaaactgtgg aggaaaccat caagaacgaa tttcctacat
      841 tgaaggttca acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc
      901 acctaaatgg tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct
      961 ccgttatccc aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca
     1021 agaacaccgc atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga
     1081 ataaggtcaa ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga
     1141 acttgcctga agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta
     1201 tcagaactgg tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg
     1261 aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa
     1321 actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga ataaataccc
     1381 gcgcttaaag ggtaatttcg aaaacagcga gtttatcatt atcaatactg ccatttcaaa
     1441 gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa aaaattagcc
     1501 ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca cagaatatat
     1561 aacatcgtag gtgtctgggt gaacagttta ttcctggcat ccactaaata taatggagcc
     1621 cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt
     1681 caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa
     1741 acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg
     1801 acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt
     1861 ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta
     1921 ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa ttctgtaaat
     1981 ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac
     2041 accaagaact tagtttcgaa taaacacaca taaacaaaca aaatggtttc cggagtcggg
     2101 ggtagtggcg gtggccgtgg cggtggccgt ggcggagaag aagaaccgtc gtcaagtcac
     2161 actcctaata accgaagagg aggagaacaa gctcaatcgt cgggaacgaa atctctcaga
     2221 ccaagaagca acactgaatc aatgagcaaa gcaattcaac agtacaccgt cgacgcaaga
     2281 ctccacgccg ttttcgaaca atccggcgaa tcagggaaat cattcgacta ctcacaatca
     2341 ctcaaaacga cgacgtacgg ttcctctgta cctgagcaac agatcacagc ttatctctct
     2401 cgaatccagc gaggtggtta cattcagcct ttcggatgta tgatcgccgt cgatgaatcc
     2461 agtttccgga tcatcggtta cagtgaaaac gccagagaaa tgttagggat tatgcctcaa
     2521 tctgttccta ctcttgagaa acctgagatt ctagctatgg gaactgatgt gagatctttg
     2581 ttcacttctt cgagctcgat tctactcgag cgtgctttcg ttgctcgaga gattaccttg
     2641 ttaaatccgg tttggataca ttccaagaat actggtaaac cgttttacgc cattcttcat
     2701 aggattgatg ttggtgttgt tattgattta gagccagcta gaactgaaga tcctgcgctt
     2761 tctattgctg gtgctgttca atcgcagaaa ctcgcggttc gtgcgatttc tcagttacag
     2821 gctcttcctg gtggagatat taagcttttg tgtgacactg tcgtggaaag tgtgagggac
     2881 ttgactggtt atgatcgtgt tatggtttat aagtttcatg aagatgagca tggagaagtt
     2941 gtagctgaga gtaaacgaga tgatttagag ccttatattg gactgcatta tcctgctact
     3001 gatattcctc aagcgtcaag gttcttgttt aagcagaacc gtgtccgaat gatagtagat
     3061 tgcaatgcca cacctgttct tgtggtccag gacgataggc taactcagtc tatgtgcttg
     3121 gttggttcta ctcttagggc tcctcatggt tgtcactctc agtatatggc taacatggga
     3181 tctattgcgt ctttagcaat ggcggttata atcaatggaa atgaagatga tgggagcaat
     3241 gtagctagtg gaagaagctc gatgaggctt tggggtttgg ttgtttgcca tcacacttct
     3301 tctcgctgca taccgtttcc gctaaggtat gcttgtgagt ttttgatgca ggctttcggt
     3361 ttacagttaa acatggaatt gcagttagct ttgcaaatgt cagagaaacg cgttttgaga
     3421 acgcagacac tgttatgtga tatgcttctg cgtgactcgc ctgctggaat tgttacacag
     3481 agtcccagta tcatggactt agtgaaatgt gacggtgcag catttcttta ccacgggaag
     3541 tattacccgt tgggtgttgc tcctagtgaa gttcagataa aagatgttgt ggagtggttg
     3601 cttgcgaatc atgcggattc aaccggatta agcactgata gtttaggcga tgcggggtat
     3661 cccggtgcag ctgcgttagg ggatgctgtg tgcggtatgg cagttgcata tatcacaaaa
     3721 agagactttc ttttttggtt tcgatctcac actgcgaaag aaatcaaatg gggaggcgct
     3781 aagcatcatc cggaggataa agatgatggg caacgaatgc atcctcgttc gtcctttcag
     3841 gcttttcttg aagttgttaa gagccggagt caaccatggg aaactgcgga aatggatgcg
     3901 attcactcgc tccagcttat tctgagagac tcttttaaag aatctatgga tcccattcgt
     3961 tcgcgcacac caagtcctgc ccgcgagctt ctgcccggac cccaacccga tggggttcag
     4021 ccgactgcag atcgtggggt gtctccgcct gccggcggcc ccctggatgg cttgcccgct
     4081 cggcggacga tgtcccggac ccggctgcca tctccccctg ccccctcacc tgcgttctcg
     4141 gcgggcagct tcagtgacct gttacgtcag ttcgatccgt cactttttaa tacatcgctt
     4201 tttgattcat tgcctccctt cggcgctcac catacagagg ctgccacagg cgagtgggat
     4261 gaggtgcaat cgggtctgcg ggcagccgac gcccccccac ccaccatgcg cgtggctgtc
     4321 actgccgcgc ggccgccgcg cgccaagccg gcgccgcgac gacgtgctgc gcaaccctcc
     4381 gacgcttcgc cggccgcgca ggtggatcta cgcacgctcg gctacagcca gcagcaacag
     4441 gagaagatca aaccgaaggt tcgttcgaca gtggcgcagc accacgaggc actggtcggc
     4501 catgggttta cacacgcgca catcgttgcg ctcagccaac acccggcagc gttagggacc
     4561 gtcgctgtca agtatcagga catgatcgca gcgttgccag aggcgacaca cgaagcgatc
     4621 gttggcgtcg gcaaacagtg gtccggcgca cgcgccctgg aggccttgct cacggtggcg
     4681 ggagagttga gaggtccacc gttacagttg gacacaggcc aacttctcaa gattgcaaaa
     4741 cgtggcggcg tgaccgcagt ggaggcagtg catgcatggc gcaatgcact gacgggtgcc
     4801 cccctgaacc ttacgccgca gcaggtggtg gccatcgcca gccacgatgg cggcaagcag
     4861 gcgctggaga cggtgcagcg gctgcttccg gtgctgtgcc aggcccatgg cctgaccccg
     4921 cagcaggtgg tggccatcgc cagcaatggc ggtggcaagc aggcgctgga gacggtgcag
     4981 cgattgttgc cggtgctgtg ccaggcccat ggcctgaccc cggagcaggt ggtggccatc
     5041 gccagcaata ttggtggcaa gcaggctctg gagacggtgc agcggctgtt gccggtgctg
     5101 tgccaggccc atggcctgac cccggagcag gtggtggcca tcgccagcaa tattgggggc
     5161 aagcaggcgc tggagacggt gcagcggctg ttgccggtgc tgtgccaggc ccatggcctg
     5221 accccgcagc aggtggtggc catcgccagc aataagggcg gcaagcaggc gctggagacg
     5281 gtgcaggcgc tgttgccggt gctgtgccag gcccatggcc tgaccccgga gcaggtggtg
     5341 gccatcgcaa gcaatattgg tggcaagcag gcgctggaga cggtgcaggc gctgttgccg
     5401 gtgctgtgcc aggcccatgg cctgaccccg gagcaggtgg tggcaatcgc cagcaatggc
     5461 ggtggcaagc aggcgctgga gacggtgcag cggctgttgc cggtgctgtg ccaggcccat
     5521 ggcctgaccc cgcaacaggt ggtagccatc gccagccacg acggtggcaa gcaggcgctg
     5581 gagacggtgc agcggctgtt gccggtgctg tgccaggccc atggcctgac accccagcag
     5641 gtggtagcga tcgccagcaa tggcggtggc aagcaggcgc tggagacggt gcagcggctg
     5701 cttccggtgc tgtgccaggc ccatggcctg accccgcagc aggtggtggc catcgccagc
     5761 aatggcggtg gcaagcaggc gctggagacg gtgcagcgat tgttgccggt gctgtgccag
     5821 gcccatggcc tgaccccgga gcaggtggtg gccatcgcca gcaataaggg tggcaagcag
     5881 gcgctggaga ctgtccagcg gctgttgccg gtgctgtgcc aggcccatgg cctgaccccg
     5941 gagcaggtgg tggccatcgc cagcaatggc ggtggcaagc aggcgcttga gacggtgcag
     6001 cggctgttgc cggtgctgtg ccaggcccat ggcctgaccc cggagcaggt ggtggccatc
     6061 gccagcaata agggtggcaa gcaggctctg gagacggtgc agcggctgtt gccggtgctg
     6121 tgccaggccc atggcctgac cccggagcag gtggtggcca tcgccagcaa tggcgggggc
     6181 aagcaggcgc tggagacggt gcagcggctg ttgccggtgc tgtgccaggc ccatggcctg
     6241 accccgcagc aggtggtggc catcgccagc aataagggcg gcaagcaggc gctggagacg
     6301 gtgcaggcgc tgttgccggt gctgtgccag gcccatggcc tgacacccca gcaggtggtg
     6361 gccatcgcca gccatgacgg cggcaggccg gcgctggaga gcattgttgc ccagttatct
     6421 cgccctgatc cggcgttggc cgcgttgacc aacgaccacc tcgtcgcctt ggcctgcctc
     6481 ggcggacgtc ctgcgctgga tgcagtgaaa aagggattgc cgcacgcgcc ggccttgatc
     6541 aaaagaacca atcgccgtat tcccgaacgc acatcccatc gcgttgccga ccacgcgcaa
     6601 gtggttcgcg tgctgggttt tttccagtgc cactcccacc cagcgcaagc atttgatgac
     6661 gccatgacgc agttcgggat gagcaggcac gggttgttac agctctttcg cagagtgggc
     6721 gtcaccgaac tcgaagcccg cagtggaacg ctccccccag cctcgcagcg ttgggaccgt
     6781 atcctccagg catcagggat gacataggtg aatttacttt aaatcttgca tttaaataaa
     6841 ttttcttttt atagctttat gacttagttt caatttatat actattttaa tgacattttc
     6901 gattcattga ttgaaagctt tgtgtttttt cttgatgcgc tattgcattg ttcttgtctt
     6961 tttcgccaca tgtaatatct gtagtagata cctgatacat tgtggatgct gagtgaaatt
     7021 ttagttaata atggaggcgc tcttaataat tttggggata ttggcttttt tttttaaagt
     7081 ttacaaatga attttttccg ccaggataac gattctgaag ttactcttag cgttcctatc
     7141 ggtacagcca tcaaatcatg cctataaatc atgcctatat ttgcgtgcag tcagtatcat
     7201 ctacatgaag tgtccggtgt cccaaatggt taccaagtgc acccagtaag ctactatgaa
     7261 agactttaca aagaactcga agagttccag aatgaaataa aaattaaaac agttgtgaat
     7321 aacagaagac aatcgcgaac tccaaaatga gctatcaaaa acgatagatc gattaggatg
     7381 actttgaaat gactccgcag tggactggcc gttaatttca agcgtgagta aaatagtgca
     7441 tgacaaaaga tgagctaggc ttttgtaaaa atatcttacg ttgtaaaatt ttagaaatca
     7501 ttatttcctt catatcattt tgtcattgac cttcagaaga aaagagccga ccaataatat
     7561 aaataaataa ataaaaataa tattccatta tttctaaaca gattcaatac tcattaaaaa
     7621 actatatcaa ttaatttgaa ttaactttat aacatatcga gatcgaaatc gtccagagca
     7681 tcggagccga gcatgtccag atcaaagtca tccaatgcgt cgcttcccaa catatccagg
     7741 tcaaagtcat cgagggcatc agaacccagc atgtcgagat cgaaatcgtc cagcgcgtcg
     7801 gcgcgcccgc ttccaccgcc tcctgcagcc ttgtcatcgt catccttgta atcgatgtca
     7861 tgatctttat aatcaccgtc atggtctttg tagtcgctcg agactttcct cttcttcttg
     7921 ggcgacgatc cacaaaactg atcagaagac ccctgttttc tctttattgg tttcaagtta
     7981 ctgttgttat tcgtcgttgt ttgatcttta gaaccagata aatctctagt cgagccattg
     8041 ccaatcgtcg aagaaccggc aaagatacca ccaccagaga cacgtggaat acccatcgcc
     8101 actggttgtt gttgcatccc cgagacttgg aactgtggtc cgtggttaac cgatgaacca
     8161 ccacggctca aatcaggcaa gcccattgca taaggcattc ccatacccat tgccattgca
     8221 gcagctgctg ccgggtaatg ccccataccc ggtgggaaca taaccgccgg tggcagatag
     8281 taaccagacg ccattgacat gatctgcact tgaagttgga gtgacttgag atactcgatg
     8341 gcttcatcta gcatcgaagc tttgtccacc ttgttacagt ttggaatgag ttcttgcagg
     8401 gcacgcatct tctcgttgat cctatcacgc cgtctccttt cagacagatt atgcacttct
     8461 gcagagcggc ttctctttga acccaaaccc gttcgagatg gacctgcttc ctttcttcca
     8521 tctcctgatt cttcttccac atcttcacta tgacagtcaa tgtcttgaat attcgaatgc
     8581 tttctcttta aagaaagtga aggactttcg gatgggccat cgagactatt acccgagcca
     8641 acagaagaac atacaactgc tttttcactc tcttggtctt ttctacatga gtcctctgat
     8701 attaggcaag ccttttggtt atctttaggc gtagcagaag caacagactc gttaagaacc
     8761 ttatcttcag agtctttagc tccaagaact ctggtctgaa acacatttgg cgggctttga
     8821 ggactctttt ctttagtgtc atgaaggtta ttattagtag tcttcgcaaa agttgcaggg
     8881 cgtaagaaat gtgaaaagtt gaccaaacta ggcttgttac tagtaattag tggttcctgc
     8941 gtctgagtaa accgatccgg attggtttgt tggctaggaa gatctctagc tctatcactt
     9001 ccatacagag aatgcgattg gaaaccgtta tactgcgacg aagaagcagc aggagctgat
     9061 tcattgccat cctttcttct ctgaaacaac gggaaagcag tttggtttac cgccatatca
     9121 ctctcttgct cgttgacagt aacaggagac gacacatcac gcaagaaatc agagcaatat
     9181 ccatcaaggg agggatgatg attcaaccat ggaacaaagt catcgtcttg actcaaaccc
     9241 gtcattagtg atggcactga catagggatc tcgtccacca tagtcgtctt tgagccattt
     9301 ccaatctctc tagctctaga agagtttgct tgtggtggag gaatgttcct cgatctactt
     9361 gactgacttt gagttgatat ctgaccattt tcccacacca gctccacaac ttcatctaca
     9421 ggtggagaag ggttcctgtc ttgagcagat tcaagcttag ctttggtgag cctgaaaagc
     9481 tcaaacagag gcattttgaa tatgtattac ttggttatgg ttatatatga caaaagaaaa
     9541 agaagaacag aagaataacg caaggaagaa caataactga aattgataga gaagtattat
     9601 gtctttgtct ttttataata aatcaagtgc agaaatccgt tagacaacat gagggataaa
     9661 atttaacgtg ggcgaagaag aaggaaaaaa gtttttgtga gggcgtaatt gaagcgatct
     9721 gttgattgta gatttttttt ttttgaggag tcaaagtcag aagagaacag acaaatggta
     9781 ttaaccatcc aatacttttt tggagcaacg ctaagctcat gcttttccat tggttacgtg
     9841 ctcagttgtt agatatggaa agagaggatg ctcacggcag cgtgactcca attgagcccg
     9901 aaagagagga tgccacgttt tcccgacggc tgctagaatg gaaaaaggaa aaatagaaga
     9961 atcccattcc tatcattatt tacgtaatga cccacacatt tttgagattt tcaactatta
    10021 cgtattacga taatcctgct gtcattatca ttattatcta tatcgacgta tgcaacgtat
    10081 gtgaagccaa gtaggcaatt atttagtact gtcagtattg ttattcattt cttagggctt
    10141 gcgtcagctt gatgaggatt tgtgatagta gccgttggag gcatgaaggg cattggaaac
    10201 atggggaaag gaggtatacc gaaaggtaac ggtactggtg gcactggcgt accgccgtta
    10261 tttacagaag ttggaaggct ggtattgttg ttcaagccag cggtgccagt tggaagttgt
    10321 aacgccatcc gcacattgtt ggctggtgac cggtaggtgt acaaattacc ctgttatccc
    10381 taactagtgg atcatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    10441 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    10501 ttcccttcct ttctcgccac gttcgccggc tttcccctgt agagaccaca tcatccacgg
    10561 ttctatactg ttgacccaat gcgtctccct tgtcatctaa acccacaccg ggtgtcataa
    10621 tcaaccaatc gtaaccttca tctcttccac ccatgtctct ttgagcaata aagctgataa
    10681 caaaatcttt gtcgctcttc gcaacgtcaa cagtaccctt agtatattct ccagtagata
    10741 gggagccctt gcatgacaat tctgctaaca tcaaaagggt ttaaaccctc taggttcctt
    10801 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
    10861 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
    10921 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
    10981 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agataactgt
    11041 attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca
    11101 gttattaccc attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
    11161 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
    11221 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
    11281 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
    11341 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
    11401 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
    11461 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    11521 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    11581 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    11641 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    11701 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    11761 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    11821 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    11881 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    11941 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    12001 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    12061 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    12121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    12181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    12241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    12301 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    12361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    12421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    12481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    12541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    12601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    12661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    12721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    12781 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    12841 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    12901 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    12961 cgttggccga ttcattaatg cagggccgta gggataacag ggtaatatta tgaatttcat
    13021 ttataaagtt tatgtacaaa tatcataaaa aaagagactc aggtatcgta agatgcaaga
    13081 gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca cacaggggcg
    13141 ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag aggtcgcctg
    13201 acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag agcgccgga
//
