LOCUS Exported 2602 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION AAV expression of GFP spaghetti monster myc construct. ACCESSION . VERSION . KEYWORDS pAAV.CAG.GFPsm-myc.WPRE.SV40 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2602) AUTHORS Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger LL TITLE High-performance probes for light and electron microscopy. JOURNAL Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 2015 Apr 27. PUBMED 25915120 REFERENCE 2 (bases 1 to 2602) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2602 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 388..410 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 472..491 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" regulatory 527..536 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 536..565 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 569..598 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 602..631 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1163..1192 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1199..1228 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1241..1270 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1277..1306 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1520..1549 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1553..1582 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1586..1615 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" misc_feature 1635..2223 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(1688..1708) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2106..2117) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 2266..2387 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2277..2296) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2331..2350 /label=SV40pA-R /note="SV40 polyA, reverse primer" ORIGIN 1 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc 61 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc 121 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg 181 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa 241 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg 301 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct 361 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta 421 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc 481 tggttattgt gctgtctcat cattttggca aagaattgga tcggccgcca ccatggagca 541 aaaactgata tcggaggaag acttggcaga gcagaaactg atttcggaag aggatcttgc 601 cgaacaaaaa ctgatcagtg aggaagacct tgccggagtg tcgaagggtg aagagctgtt 661 caccggcgtg gtgcccatcc tcgtcgagct ggacggagat gtcaatggac ataaattctc 721 cgtacgggga gagggcgagg gcgacgcaac caacggtaaa ctgaccctga agttcatttg 781 caccacgggt aaactgcccg taccctggcc caccctggtc acaaccttgg gtggcggagt 841 tcagtgtttt tcgcggtatc cagatcacat gaagcagcat gactttttca agagcgctat 901 gcccgagggt tacgttcaag agaggaccat cagtttcaag gacgacggca cctacaagac 961 acgggctgag gtgaaattcg agggtgatac actcgtgaat cgtatcgagc tgaagggcat 1021 agattttaag gaggatggca atatcctggg ccacaagttg gagtataact tcaacagtca 1081 caatgtctat ataactgccg ataagcagaa gaatggcatt aaagccaatt tcaaaattcg 1141 tcacaatgtc gaaggcggcg cagaacagaa gctcatcagt gaagaggatc tggccgcaga 1201 gcagaaactc attagcgagg aggacttggg cggaggcggc gagcagaagc tgatatcgga 1261 ggaagacctg gcggccgagc aaaagctcat ctcggaggag gatctcgcgg gaggagatgg 1321 aagtgtccag ttggcggatc actaccagca gaatacgcca attggtgacg gccccgtcct 1381 tctgccggat aaccactacc tctccaccca gtcggtgctg agcaaggacc cgaatgaaaa 1441 acgtgaccac atggttcttc ttgagttcgt cacggctgcg ggcatcacgt tgggcatgga 1501 cgaactgtac aagggcgctg aacagaaact tatctccgag gaggatctgg cggaacagaa 1561 gctgatcagc gaagaggacc tggcggagca gaaacttatc agcgaagagg atctgtaaac 1621 cggagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 1681 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 1741 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 1801 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 1861 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 1921 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 1981 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 2041 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 2101 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 2161 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 2221 cgcatcgata ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg 2281 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg 2341 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt 2401 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa 2461 acctctacaa atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag 2521 tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc 2581 tctctgcgcg ctcgctcgct ca //