LOCUS Exported 9694 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of BMP4. ACCESSION . VERSION . KEYWORDS BMP4_pLX307 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9694) AUTHORS Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe J, Lage K, Boehm JS, Hahn WC TITLE Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in beta-Catenin-Active Cancers. JOURNAL Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 10.1016/j.cels.2016.09.001. PUBMED 27684187 REFERENCE 2 (bases 1 to 9694) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9694 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 98..1276 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 329..1267 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1224..1244 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1296..1320 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2545..2569) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2577..2618 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2729..3317 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2782..2802) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3200..3211) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3389..3622 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3700..3821 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3737..3756) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3791..3810 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 3861..3996 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(4016..4035) /label=T7 /note="T7 promoter, forward primer" promoter complement(4017..4035) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4045..4062) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4045..4061) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4054..4076) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4203..4658 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4290..4309) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4500..4521 /label=F1ori-F /note="F1 origin, forward primer" promoter 4684..4788 /gene="bla" /label=AmpR promoter CDS 4789..5649 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5007..5026) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5820..6408 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6309..6328 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6562..6579 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6696..6717 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6732..6762 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6770..6786 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6775..6797 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6794..6810 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6794..6810 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6829..6849 /label=T3 /note="T3 promoter, forward primer" promoter 6831..6849 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 6877..7103 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7104..7284 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7331..7456 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7949..8182 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8367..8411 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 8642..8852 /label=SV40 promoter /note="SV40 early promoter" promoter 8664..8860 /label=SV40 promoter /note="SV40 early promoter" rep_origin 8711..8846 /label=SV40 ori /note="SV40 origin of replication" CDS 8892..9494 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(8892..8911) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9388..9408 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 9537..9654 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag 61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc 121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt 421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca 1321 tgattcctgg taaccgaatg ctgatggtcg ttttattatg ccaagtcctg ctaggaggcg 1381 cgagccatgc tagtttgata cctgagacgg ggaagaaaaa agtcgccgag attcagggcc 1441 acgcgggagg acgccgctca gggcagagcc atgagctcct gcgggacttc gaggcgacac 1501 ttctgcagat gtttgggctg cgccgccgcc cgcagcctag caagagtgcc gtcattccgg 1561 actacatgcg ggatctttac cggcttcagt ctggggagga ggaggaagag cagatccaca 1621 gcactggtct tgagtatcct gagcgcccgg ccagccgggc caacaccgtg aggagcttcc 1681 accacgaaga acatctggag aacatcccag ggaccagtga aaactctgct tttcgtttcc 1741 tctttaacct cagcagcatc cctgagaacg aggcgatctc ctctgcagag cttcggctct 1801 tccgggagca ggtggaccag ggccctgatt gggaaagggg cttccaccgt ataaacattt 1861 atgaggttat gaagccccca gcagaagtgg tgcctgggca cctcatcaca cgactactgg 1921 acacgagact ggtccaccac aatgtgacac ggtgggaaac ttttgatgtg agccctgcgg 1981 tccttcgctg gacccgggag aagcagccaa actatgggct agccattgag gtgactcacc 2041 tccatcagac tcggacccac cagggccagc atgtcaggat tagccgatcg ttacctcaag 2101 ggagtgggaa ttgggcccag ctccggcccc tcctggtcac ctttggccat gatggccggg 2161 gccatgcctt gacccgacgc cggagggcca agcgtagccc taagcatcac tcacagcggg 2221 ccaggaagaa gaataagaac tgccggcgcc actcgctcta tgtggacttc agcgatgtgg 2281 gctggaatga ctggattgtg gccccaccag gctaccaggc cttctactgc catggggact 2341 gcccctttcc actggctgac cacctcaact caaccaacca tgccattgtg cagaccctgg 2401 tcaattctgt caattccagt atccccaaag cctgttgtgt gcccactgaa ctgagtgcca 2461 tctccatgct gtacctggat gagtatgata aggtggtact gaaaaattat caggagatgg 2521 tagtagaggg atgtgggtgc cgctgcccaa ctttcttgta caaagtggtt gatatcggta 2581 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact agtccgtaac 2641 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat ccgatcgtac 2701 tgatttaata aatacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag 2761 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 2821 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 2881 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 2941 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 3001 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 3061 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 3121 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 3181 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 3241 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 3301 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt 3361 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 3421 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 3481 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 3541 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 3601 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat 3661 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 3721 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 3781 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 3841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 3961 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta 4021 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa 4081 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 4141 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 4201 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 4261 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 4321 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 4381 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 4441 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 4501 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 4561 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 4621 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 4681 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 4741 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 4801 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 4861 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 4921 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 4981 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 5041 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 5101 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 5161 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 5221 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 5281 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 5341 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 5401 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 5461 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 5521 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 5581 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 5641 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 5701 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 5761 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 5821 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 5881 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 5941 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 6001 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 6061 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 6121 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 6181 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 6241 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 6301 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 6361 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 6421 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 6481 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 6541 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 6601 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 6661 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 6721 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 6781 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct 6841 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt 6901 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc 6961 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga 7021 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta 7081 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc 7141 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 7201 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 7261 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa 7321 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga 7381 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga 7441 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg 7501 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga 7561 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat 7621 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa 7681 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc 7741 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc 7801 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat 7861 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg 7921 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag 7981 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt 8041 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt 8101 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat 8161 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca 8221 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca 8281 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa 8341 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat 8401 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca 8461 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga 8521 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg 8581 gacccatgca ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 8641 agcaggcaga agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct 8701 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 8761 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 8821 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac 8881 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 8941 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 9001 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 9061 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga 9121 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 9181 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 9241 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 9301 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 9361 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 9421 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 9481 ccggtgccag ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt 9541 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca 9601 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt 9661 tattacaggg acagcagaga tccactttgg cgcc //