LOCUS       Exported               10000 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  High copy number plasmid 1 to prepare Penn State DNA molecular 
            weight ladders.
ACCESSION   .
VERSION     .
KEYWORDS    pPSU1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Henrici RC, Pecen TJ, Johnston JL, Tan S
  TITLE     The pPSU Plasmids for Generating DNA Molecular Weight Markers.
  JOURNAL   Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1.
  PUBMED    28550309
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     112..131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     365..382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    499..520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        535..565
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    573..589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     578..600
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     597..613
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     597..613
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7997..8014)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7997..8013)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8006..8028)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8222..8241)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8341..8363
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8401..8419)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8487..8591
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8592..9452
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8810..8829)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9623..211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
       61 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
      361 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
      421 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      481 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      541 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      601 aaacagctat gaccatgatt acgccaagct tgatatcatc aaagccatga acaaagcagc
      661 cgcgctggat gaactgatac cggggttgct gagtgaatat atcgaacagt caggttaaca
      721 ggctgcggca ttttgtccgc gccgggcttc gctcactgtt caggccggag ccacagaccg
      781 ccgttgaatg ggcggatgct aattactatc tcccgaaaga atccgcatac caggaagggc
      841 gctgggaaac actgcccttt cagcgggcca tcatgaatgc gatgggcagc gactacatcc
      901 gtgaggtgaa tgtggtgaag tctgcccgtg tcggttattc caaaatgctg ctgggtgttt
      961 atgcctactt tatagagcat aagcagcgca acacccttat ctggttgccg acggatggtg
     1021 atgccgagaa ctttatgaaa acccacgttg agccgactat tcgtgatatt ccgtcgctgc
     1081 tggcgctggc cccgtggtat ggcaaaaagc accgggataa cacgctcacc agatatcgaa
     1141 gcctacgcgc tgaacgccag cggtgtggtg aatatcatcg tgttcgatcc gaaaggctgg
     1201 gcgctgttcc gttccttcaa agccgtcaag gagaagctgg atacccgtcg tggctctaat
     1261 tccgagctgg agacagcggt gaaagacctg ggcaaagcgg tgtcctataa ggggatgtat
     1321 ggcgatgtgg ccatcgtcgt gtattccgga cagtacgtgg aaaacggcgt caaaaagaac
     1381 ttcctgccgg acaacacgat ggtgctgggg aacactcagg cacgcggtct gcgcacctat
     1441 ggctgcattc aggatgcgga cgcacagcgc gaaggcatta acgcctctgc ccgttacccg
     1501 aaaaactggg tgaccaccgg cgatccggcg cgtgagttca ccatgattca gtcagcaccg
     1561 ctgatgctgc tggctgaccc tgatgagttc gtgtccgtac aactggcgta atcatggccc
     1621 ttcggggcca ttgtttctct gtggaggagt ccatgacgaa agatgaactg attgcccgtc
     1681 tccgctcgct gggtgaacaa ctgaaccgtg atgtcagcct gacggggacg aaagaagaac
     1741 tggcgctccg tgtggcagag ctgaaagagg agcttgatga cacggatgaa actgccggtc
     1801 aggacacccc tctcagccgg gaaaatgtgc tgaccggaca tgaaaatgag gtgggatcag
     1861 cgcagccgga taccgtgatt ctggatacgt ctgaactggt cacggtcgtg gcactggtga
     1921 agctgcatac tgatgcactt cacgccacgc gggatgaacc tgtggcattt gtgctgccgg
     1981 gaacggcgtt tcgtgtctct gccggtgtgg cagccgaaat gacagagcgc ggcctggcca
     2041 gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgtctgcag ctgccattgc
     2101 ccgcgccgat gaaacgatac gcgggtacat ggatatcggt acccttcatt tcgcataaat
     2161 caccaactcg ttgcccggta acaacagcca gttccattgc aagtctgagc caacatggtg
     2221 atgattctgc tgcttgataa attttcaggt attcgtcagc cgtaagtctt gatctcctta
     2281 cctctgattt tgctgcgcga gtggcagcga catggtttgt tgttatatgg ccttcagcta
     2341 ttgcctctcg gaatgcatcg ctcagtgttg atctgattaa cttggctgac gccgccttgc
     2401 cctcgtctat gtatccattg agcattgccg caatttcttt tgtggtgatg tcttcaagtg
     2461 gagcatcagg cagacccctc cttattgctt taattttgct catgtaattt atgagtgtct
     2521 tctgcttgat tcctctgctg gccaggattt tttcgtagcg atcaagccat gaatgtaacg
     2581 taacctgcag atcactgttg attctcgctg tcagaggctt gtgtttgtgt cctgaaaata
     2641 actcaatgtt ggcctgtata gcttcagtga ttgcgattcg cctgtctctg cctaatccaa
     2701 actctttacc cgtccttggg tccctgtagc agtaatatcc attgtttctt atataaaggt
     2761 tagggggtaa atcccggcgc tcatgacttc gccttcttcc catttctgat cctcttcaaa
     2821 aggccacctg ttactggtcg atttaagtca acctttaccg ctgattcgtg gaacagatac
     2881 tctcttccat ccttaaccgg aggtgggaat atcctgcatt cccgaaccca tcgacgaact
     2941 gtttcaaggc ttcttggacg tcgctggcgt gcgttccact cctgaagtgt caagtacatc
     3001 gcaaagtctc cgcaattaca cgcaagaaaa aaccgccatc aggcggcttg gtgttctttc
     3061 agttcttcaa ttcgaatatt ggttacgtct gcatgtgcta tctgcgccca tatcatccag
     3121 tggtcgtagc agtcgttgat gttctccgct tcgataactc tgttgaatgg ctctccattc
     3181 cattctcctg tgactcggaa gtgcatttat catctccata aaacaaaacc cgccgtagcg
     3241 agttcagata aaataaatcc ccgcgagtgc gaggattgtt atgtaatatt gggtttaatc
     3301 atctatatgt tttgtacaga gagggcaagt atcgtttcca ccgtactcgt gataataatt
     3361 ttgcacggta tcagtcattt ctcgcacatt gcagaatggg gatttgtctt cattagactt
     3421 ataaaccttc atggaatatt tgtatgccga ctctatatct ataccttcat ctacataaac
     3481 accttcgtga tgtctgcatg gagacaagac accggatctg cacaacattg ataacgccca
     3541 atctttttgc tcagactcta actcattgat actcatttat aaactccttg caatgtatgt
     3601 cgtttcagct aaacggtatc agcaatgttt agatatctct agacttccga ttagaaacgt
     3661 caaggcagca atcaggattg caatcatggt tcctgcatat gatgacaatg tcgccccaag
     3721 accatctcta tgagctgaaa aagaaacacc aggaatgtag tggcggaaaa ggagatagca
     3781 aatgcttacg ataacgtaag gaattattac tatgtaaaca ccaggcatga ttctgttccg
     3841 cataattact cctgctaatt aatccttaac tttgcccacc tgccttttaa aacattccag
     3901 tatatcactt ttcattcttg cgtagcaata tgccatctct tcagctatct cagcattggt
     3961 gaccttgttc agaggcgctg agagatggcc tttttctgat agataatgtt ctgttaaaat
     4021 atctccggcc tcatcttttg cccgcaggct aatgtctgaa aattgaggtg acgggttaaa
     4081 aataatatcc ttggcaacct tttttatatc ccttttaaat tttggcttaa tgactatatc
     4141 caatgagtca aaaagctccc cttcaatatc tgttgcccct aagaccttta atatatcgcc
     4201 aaatacaggt agcttggctt ctaccttcac cgttgttcgg ccgatgaaat gcatatgcat
     4261 aacatcgtct ttggtggttc ccctcatcag tggctctatc tgaacgcgct ctccactgct
     4321 taatgacatt cctttcccga ttaaaaaatc tgtcagatcg gatgtggtcg gcccgaaaac
     4381 agttctggca aaaccaatgg tgtcgccttc aacaaacaaa aaagatggga atcccaatga
     4441 ttcgtcatct gcgaggctgt tcttaatatc ttcaactgaa gctttagagc gatttatctt
     4501 ctgaaccaga ctcttgtcat ttgttttggt aaagagaaaa gtttttccat cgatactgat
     4561 gtatcatgtc caaagaacga ttatctgcag atcgaacaac gagaatcaag atcactgagc
     4621 taaatcccca cctgatgtgt gtgctttgtg gagggtactt cattgatgcc acaaccataa
     4681 tagaatgtct acattccttc tgtaaaacgt gtattgttcg ttacctggag accagcaagt
     4741 attgtcctat ttgtgatgtc caagttcaca agaccagacc actactgaat ataaggtcag
     4801 ataaaactct ccaagatatt gtatacaaat tagttccagg gcttttcaaa aatgaaatga
     4861 agagaagaag ggatttttat gcagctcatc cttctgctga tgctgccaat ggctctaatg
     4921 aagatagagg agaggttgca gatgaagata agagaattat aactgatgat gagataataa
     4981 gcttatccat tgaattcttt gaccagaaca gattggatcg gaaagtaaac aaagacaaag
     5041 agaaatctaa ggaggaggtg aatgataaaa gatacttacg atgcccagca gcaatgactg
     5101 tgatgcactt aagaaagttt ctcagaagta aaatggacat acctaatact ttccagattg
     5161 atgtcatgta tgaggaggaa cctttaaagg attattatac actaatggat attgcctaca
     5221 tttatacctg gagaaggaat ggtccacttc cattgaaata cagagttcga cctacttgta
     5281 aaagaatgaa gatcagtcac cagagagatg gactgacaaa tgctggagaa ctggaaagtg
     5341 actctgggag tgacaaggcc aacagcccag caggaggtat tccctccacc tcttcttgtt
     5401 tgcctagccc cagtactcca gtgcagtctc ctcatccaca gtttcctcac atttccagta
     5461 ctatgaatgg aaccagcaac agccccagcg gtaaccacca atcttctttt gccaatagac
     5521 ctcgaaaatc atcagtaaat gggtcatcag caacttcttc tggttaatgt acataatcag
     5581 atctctgcag ttgatgcgtg gatggagtcc cggatttatc cggtgatgag cgatatcccg
     5641 gcactgtcag atttgatcac cagtatggtg gccagcggct atgactaccg gcgcgacgat
     5701 gatgcgggct tgtggagttc agccgatctg acttatgtca ttacctatga aatgtgagga
     5761 cgctatgcct gtaccaaatc ctacaatgcc ggtgaaaggt gccgggacca ccctgtgggt
     5821 ttataagggg agcggtgacc cttacgcgaa tccgctttca gacgttgact ggtcgcgtct
     5881 ggcaaaagtt aaagacctga cgcccggcga actgaccgct gagtcctatg acgacagcta
     5941 tctcgatgat gaagatgcag actggactgc gaccgggcag gggcagaaat ctgccggaga
     6001 taccagcttc acgctggcgt ggatgcccgg agagcagggg cagcaggcgc tgctggcgtg
     6061 gtttaatgaa ggcgataccc gtgcctataa aatccgcttc ccgaacggca cggtcgatgt
     6121 gttccgtggc tgggtcagca gtatcggtaa ggcggtgacg gcgaaggaag tgatcacccg
     6181 cacggtgaaa gtcaccaatg tgggacgtcc gtcgatggca gaagatcgca gcacggtaac
     6241 agcggcaacc ggcatgaccg tgacgcctgc cagcacctgg atctctgcag ccatggagat
     6301 catctgccgg gacgtggctc ggggctatga gaacgtgccc attccctgtg tcaacggtgt
     6361 ggatggggag ccctgccctg aggattacaa gtacatctca gagaactgcg agacgtccac
     6421 catgaacatc gatcgcaaca tcacccacta acagcactgc acgtgtgtgg acgactgctc
     6481 tagctccaac tgcctgtgcg gccagctcag catccggtgc tggtatgaca aggatgggcg
     6541 attgctccag gaatttaaca agattgagcc tccgctgatt ttcgagtgta accaggcgtg
     6601 ctcatgctgg agaaactgca agaaccgggt cgtacagagt ggcatcaagg tgcggctaca
     6661 gctctaccga acagccaaga tgggctgggg ggtccgcgcc ctgtaaacca tcccacaggg
     6721 gaccttcatc tgcgagtatg tcggggagct gatctctgat gctgaggctg atgtgagaga
     6781 ggatgattct tacctcttcg acttagacaa caaggatgga gaggtgtact gcatagatgc
     6841 ccgttactat ggcaacatca gccgcttcat caaccacctg tgtgacccca acatcattcc
     6901 cgtccgggtc ttcatgctgc accaagacct gcgatttcca cgcatcgcct tcttcagttc
     6961 ccgagacatc cggactgggg aggagctagg gtttgactat ggcgaccgct tctgggacat
     7021 caaaagcaaa tatttcacct gccaatgtgg ctctgagaag tgcaagcact cagggcccgg
     7081 atctctgcag gtgcagagtg atttgccgtg gtggctggtc tgccggggga cgattcataa
     7141 gttccgctgt gtgccgcatc tcaccgggcg gcgctttgag cacggtgtga cggactgtta
     7201 cacactgttc cgggatgctt atcatctggc ggggattgag atgccggact ttcatcgtga
     7261 ggatgactgg tggcgtaacg gccagaatct ctatctggat aatctggagg cgacggggct
     7321 gtatcaggtg ccgttgtcag cggcacagcc gggcgatgtg ctgctgtgct gttttggttc
     7381 atcagtgccg aatcacgccg caatttactg cggcgacggc gagctgctgc accatattcc
     7441 tgaacaactg agcaaacgag agaggtacac cgacaaatgg cagcgacgca cacactccct
     7501 ctggcgtcac cgggcatggc gcgcatctgc ctttacgggg atttacaacg atttggtcgc
     7561 cgcatcgacc ttcgtgtgaa aacgggggct gaagccatcc gggcactggc cacacagctc
     7621 ccggcgtttc gtcagaaact gagcgacggc tggtatcagg tacggattgc cgggcgggac
     7681 gtcagcacgt ccgggttaac ggcgcagtta catgagactc tgcctgatgg cgctgtaatt
     7741 catattgttc ccagagtcgc cggggccaag tcaggtggcg tattccagat tgtcctgggg
     7801 gctgccgcca ttgccggatc attctttacc gccggagcca cccttgcagc atggggggca
     7861 gccattgggg ccggtggtat gaccggcatc ctgttttctc tcggtgccag tatggtgctc
     7921 ggtggtgtgg cgcagatgct ggcaccgaaa gccagaactc cccgtataca gacaacggat
     7981 aacgctgcag gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8041 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8101 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     8161 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     8221 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     8281 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     8341 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     8401 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     8461 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8521 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8581 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8641 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8701 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8761 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8821 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8881 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8941 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9001 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9061 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9121 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9181 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9241 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9301 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9361 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9421 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9481 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9541 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9901 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9961 agcccagctt ggagcgaacg acctacaccg aactgagata
//
