LOCUS       Exported                9144 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Thy1 promoter construct
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Feng G, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QT, Wallace M,
            Nerbonne JM, Lichtman JW, Sanes JR
  TITLE     Imaging neuronal subsets in transgenic mice expressing multiple 
            spectral variants of GFP.
  JOURNAL   Neuron. 2000 Oct . 28(1):41-51.
  PUBMED    11086982
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7099..7118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7218..7240
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7278..7296)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7364..7468
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7469..8329
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7687..7706)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8500..9088
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8989..9008
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 gatcgcggcc gcgatccccg ggcgagctcg aattcagaga ccgggaacca aactagcctt
      241 taaaaaacat aagtacagga gccagcaaga tggctcagtg ggtaaaggtg cctaccagca
      301 agcctgacag cctgagttca gtccccacga actacgtggt aggagaggac caaccaactc
      361 tggaaatctg ttctgcaaac acatgctcac acacacacac acaaatagta taaacaattt
      421 taaatttcat ttaaaaataa tttgtaaaca aaatcattag cacaggtttt agaaagagcc
      481 tcttggtgac atcaagttga tgctgtagat ggggtatcat tcctgaggac ccaaaaccgg
      541 gtctcagcct ttccccattc tgagagttct ctcttttctc agccactagc tgaagagtag
      601 agtggctcag cactgggctc ttgagttccc aagtcctaca actggtcagc ctgactacta
      661 accagccatg aagaaacaag gagtggatgg gctgagtctg ctgggatggg agtggagtta
      721 gtaagtggcc atggatgtaa tgaccccagc aatgctggct agaaggcatg cctcctttcc
      781 ttgtctggag acggaacggg agggatcatc ttgtactcac agaagggaga acattctagc
      841 tggttgggcc aaaatgtgca agttcacctg gaggtggtgg tgcatgcttt taactccagt
      901 actcaggagg cagggccagg tggatctctg tgagttcaag accagcctgc actatggaga
      961 gagttttggg acagccagag ttacacagaa aaatcctggt ggaaaatctg aaagaaagag
     1021 agaaagaaag aaagaaagaa aggaagaaag aaagaaagag tggcaggcag gcaggcagga
     1081 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaaatagg tgcgacttca
     1141 agatccggag ttacaagcag aatgcactgt ttccctaaca gggccaagtg ttttgagtaa
     1201 ctgaaggtgg gcatgatgcc tgggaagcag aaacaagcca ggcagatgca ccccttgcct
     1261 tgcttccgaa gggctgcagt agcatggaaa acatggaaaa caaccaatcc attccctttg
     1321 ctgatataac aggctccaaa gccaaaacct gtcactggag gctcaagagc agatctccag
     1381 ccaagaggca aaggaatggg ggaagctgga gggcctccct ctggttatcc aggcttctga
     1441 aggttcaagc aaagaaaggg ttacaacctt aaaaggagag cgtcccgggg tatgggtaga
     1501 agactgctcc accccgaccc ccagggtccc taaccgtctt ttccctgggc gagtcagccc
     1561 aatcacagga ctgagagtgc ctctttagta gcagcaagcc acttcggaca cccaaatgga
     1621 acacctccag tcagccctcg ccgaccaccc caccccctcc atccttttcc ctcagcctcc
     1681 gattggctga atctagagtc cctccctgct cccccctctc tccccacccc tggtgaaaac
     1741 tgcgggcttc agcgctgggt gcagcaactg gaggcgttgg cgcaccagga ggaggctgca
     1801 gctaggggag tccaggtgag agcaggccga cgggagggac ccgcacatgc aaggaccgcc
     1861 gcagggcgag gatgcaagcc ttccccagct acagttttgg gaaaggatac cagggcgctc
     1921 ctatatgggg gcgcgggaac tggggaaaga aggtgctccc aggtcgaggt gggagaggaa
     1981 ggcagtgcgg ggtcacgggc tttctccctg ctaacggacg ctttcgaaga gtgggtgccg
     2041 gaggagaacc atgaggaagg acatcaagga cagcctttgg tccccaagct caaatcgctt
     2101 tagtggtgcg aatagaggga ggaggtgggt ggcaaactgg agggagtccc cagcgggtga
     2161 cctcgtggct ggctgggtgc ggggcaccgc aggtaagaaa accgcaatgt tgcgggaggg
     2221 gactgggtgg caggcgcggg ggaggggaaa gctagaaagg atgcgaggga gcggaggggg
     2281 gagggagcgg gagaatctca actggtagag gaagattaaa atgaggaaat agcatcaggg
     2341 tggggttagc caagccgggc ctcagggaaa gggcgcaaag tttgtctggg tgtgggctta
     2401 ggtgggctgg gtatgagatt cggggcgccg aaaacactgc tgcgcctctg ccaaatcacg
     2461 ctacccctgt atctagttct gccaggcttc tccagcccca gccccaattc ttttctctag
     2521 tgttccccct tccctcccct gaatctcaag cccacactcc ctcctccata acccactgtt
     2581 atcaaatcta agtcatttgc cacccaacaa ccatcaggag gcggaagcag acgggaggag
     2641 tttgagatca acttgggcta catcacgagt tccaggctca ccaaggcttc ttaaggagac
     2701 cttgtctcta aaattaatta attaattaat taatagtccc ctttctctgc cacagaacct
     2761 tgggatctgg ctcctggtcg cagctccccc caccccaggc tgacattcac tgccatagcc
     2821 catccggaaa tcctagtcta tttccccatg gatcttgaac tgcagagaga atggcagagt
     2881 ggcccgccct gtgcaaagga tgttcctagc ctaggtggag ctcgcgaact cgcagactgt
     2941 gcctctcttg ggcaaggaca ggctagacag cctgccggtg tgttgagcta gggcactgtg
     3001 gggaaggcag agaacctgtg cagggcagca atgaacacag gaccagaaaa ctgcagccct
     3061 aggaacactc aagagctggc catttgcaag catctctggc ctccgtgctt ctcactcatg
     3121 tcccatgtct tatacaggcc tctgtggcac ctcgcttgcc tgatctcatc cctagccgtt
     3181 aagctttctg catgacttat cacttggggc ataatgctgg atacctacca ttttcttaga
     3241 ccccatcaaa atcctatttg agtgtacggt tcggagaacc tcatttatcc ggtaaatgtc
     3301 ttttactctg ctctcaggga gctgaggcag gacatcctga gatacattgg gagaggagat
     3361 acagtttcaa taaaataata ggttgggtgg aggtacatgc ctataatgcc accactcagg
     3421 aaatggtggc agcttcgtga gtttgaggcc aacccaagaa acatagtgaa accctgtcag
     3481 taaataagta agcaagtatt tgagtatcta ctatatgcta gggctgacct ggacattagg
     3541 ggtcatcttc tgaacaaact agtgcttgag ggaggtattt ggggtttttg tttgtttaat
     3601 ggatctgaat gagttccaga gactggctac acagcgatat gactgagctt aacaccccta
     3661 aagcatacag tcagaccaat tagacaataa aaggtatgta tagcttacca aataaaaaaa
     3721 ttgtattttc aagagagtgt ctgtctgtgt agccctggct gttcttgaac tcactctgta
     3781 gaccaggctg gcctggaaat ccatctgcct gcctctgcct ctctgcctct ctgcctctct
     3841 gcctctctct ctgcctctct ctgcctctct ctgcccctct ctgcccctct ctgcccctct
     3901 ctgccgccct ctgccttttg ccctctgccc tctgttctct ggcctctgcc ctctgccctc
     3961 tggcctctgg cctctgcctc tgcctcttga gtgctggaat caaaggtgtg agctctgtag
     4021 gtcttaagtt ccagaagaaa gtaatgaagt cacccagcag ggaggtgctc agggacagca
     4081 cagacacaca cccaggacat aggctcccac ttccttggct ttctctgagt ggcaaaggac
     4141 cttaggcagt gtcactccct aagagaaggg gataaagaga ggggctgagg tattcatcat
     4201 gtgctccgtg gatctcaagc cctcaaggta aatggggacc cacctgtcct accagctggc
     4261 tgacctgtag ctttccccac cacagaatcc aagtcggaac tcttggcacc tagaggatct
     4321 cgaggtcctt cctctgcaga ggtcttgctt ctcccggtca gctgactccc tccccaagtc
     4381 cttcaaatat ctcagaacat ggggagaaac ggggaccttg tccctcctaa ggaaccccag
     4441 tgctgcatgc catcatcccc cccaccctcg cccccacccc cgccacttct ccctccatgc
     4501 ataccactag ctgtcatttt gtactctgta tttattccag ggctgcttct gattatttag
     4561 tttgttcttt ccctggagac ctgttagaac ataagggcgt atggtgggta ggggaggcag
     4621 gatatcagtc cctggggcga gttcctccct gccaaccaag ccagatgcct gaaagagata
     4681 tggatgaggg aagttggact gtgcctgtac ctggtacagt catactctgt tgaaagaatc
     4741 atcggggagg ggggggggct caagagggga gagctctgct gagcctttgt ggaccatcca
     4801 atgaggatga gggcttagat tctaccaggt cattctcagc caccacacac aagcgctctg
     4861 ccatcactga agaagccccc tagggctctt gggccagggc acactcagta aagatgcagg
     4921 ttcagtcagg gaatgatggg gaaaggggta ggaggtgggg gagggatcac cccctcctct
     4981 aaaacacgag cctgctgtct ccaaaggcct ctgcctgtag tgagggtggc agaagaagac
     5041 aaggagccag aactctgact ccaggatcta agtccgtgca ggaaggggat cctagaacca
     5101 tctggttgga cccagcttac caagggagag cctttattct tctttccctt gcccctctgt
     5161 gccagcccct cttgctgtcc ctgatccccc agacagcgag agtcttgcaa cctgcctctt
     5221 ccaagacctc ctaatctcag gggcaggcgg tggagtgaga tccggcgtgc acactttttg
     5281 gaagatagct ttcccaagga tcctctcccc cactggcagc tctgcctgtc ccatcaccat
     5341 gtataatacc accactgcta cagcatctca ccgaggaaag aaaactgcac aataaaacca
     5401 agcctctgga gtgtgtcctg gtgtctgtct cttctgtgtc ctggcgtctg tctcttctgt
     5461 gttcttccaa ggtcagaaac aaaaaccaca cacttcaacc tggatggctc ggctgagcac
     5521 ttctgtgtgc agaaggtcca accagactct ggggtacccc ggccctccct attcccttgc
     5581 ctcctgtctc ccgcttttta tagctcccta tgctgggctt ctctggagag tgaaatcttt
     5641 gcccaaatca atgcgcattc tctctgctga gtcatctggc gacagcagtt gagttcaccc
     5701 gccaacacat gggcccagct atgtagccga accctggctc tggaagtgcc agggactttg
     5761 tgcataagta tgtaccatgc ccttttttca cagtcctagc tctgcagaag tgcagcctga
     5821 aggcctgtct gctgagagga catgccctgg agccctgaaa caggcacagt gggaggagga
     5881 acggaggatg acaggcatca ggccctcagt ccaaaagcaa ccacttgaga atgggctgga
     5941 gtacgaaaca tggggtcccg tccctggatc cctcctcaaa gagtaataag taaaatataa
     6001 acaggtaccc caggccgttc tgggtttggg ttgtaatggg atccatttgc agagaactat
     6061 tgagacagcc cagccgtact gtgacaggca atgtggggga ggaggttgaa tcacttggta
     6121 tttagcatga atagaataat tccctgaaca tttttcttaa acatccatat ctaaattacc
     6181 accactcgct cccagtcttc ctgcctttgc gccagcctcc tgtctggcca tgcctgaaga
     6241 aggctggaga agccacccac ctcaggccat gacactgcca gccacttggc aggtgcagcc
     6301 aaacctgagc tgtcccagaa agggacattc tcaagaccca ggcaccctga tcagcactga
     6361 cttggagcta caagtgtcat gccagaaaag tctctaagaa aaccttttca gggaaaaggg
     6421 ggtgactcaa caccgggcaa gtttgggaag ccccaccctt cgagtgatgg aagagcagat
     6481 aggaagcctc agaagagaga caccggcacc caggtaacgt tcctcatgtg gtctctgtca
     6541 cactaggtgc tcttccctgg acatctccgt gaccacactc tcagttctta gggagatgcg
     6601 ggtgctctct gaggctatct cagagttgca gattctgagg cctagagtga ctacagtcag
     6661 cctaggaagc cacagaggac tgtggaccag gagggcagaa gaggagaagg gaagaaaaac
     6721 catcagatag gacttgcaat gaaactaacc caagacaatc ataatgcaga caggaatgtt
     6781 aaaggcgttc agcagctggc catgacaccc atctgtccct ctggccaagt cagcaagcct
     6841 ggaagacctg ggactcctgc ccatatgtcc taagctcccc acccacccac tcgttcactg
     6901 tccttattct ctctctacct tcagccactt agtttcctac cttaagtcct agaattgatc
     6961 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     7021 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     7081 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     7141 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     7201 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     7261 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     7321 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7381 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7441 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7501 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7561 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7621 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7681 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7741 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7801 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7861 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7921 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7981 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8041 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8101 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8161 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8221 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8281 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8341 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8401 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8461 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8521 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8581 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8641 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8701 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8761 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8821 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8881 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8941 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9001 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9061 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9121 ggccttttgc tggccttttg ctca
//
