LOCUS Exported 11904 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259). ACCESSION . VERSION . KEYWORDS pCMVR8.74 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11904) TITLE TRONO lab plasmids REFERENCE 2 (bases 1 to 11904) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11904 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 32..51 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 285..302 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(372..392) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 377..706 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 557..692 /label=SV40 ori /note="SV40 origin of replication" primer_bind 619..638 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 1840..1905 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 2035..2055 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" enhancer 2509..2888 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2889..3092 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 3043..3063 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 3089..3113 /label=LNCX /note="Human CMV promoter, forward primer" CDS 3230..4732 /codon_start=1 /gene="gag" /product="gag protein from human immunodeficiency virus 1" /label=HIV-1 gag /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ" CDS 4525..7536 /codon_start=1 /gene="pol" /product="pol protein from human immunodeficiency virus 1" /label=HIV-1 pol /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ MAGDDCVASRQDED" misc_feature 7221..7338 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" misc_feature 8110..8343 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8528..8572 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" CDS 8866..8892 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" promoter complement(9505..9523) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(9506..9523) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(9813..9832) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(10046..10065) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 10165..10187 /label=pGEX 3' /note="pGEX vectors, reverse primer" promoter 10311..10415 /gene="bla" /label=AmpR promoter CDS 10416..11276 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10634..10653) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11447..131 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 361 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 421 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 481 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 541 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 601 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 661 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa 721 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg 781 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc 841 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac 901 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac 961 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg 1021 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg 1081 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg 1141 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc 1201 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg 1261 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg 1321 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc 1381 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc 1441 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta 1501 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc 1561 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt 1621 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga 1681 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca 1741 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca 1801 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt 1861 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga 1921 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa 1981 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa 2041 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt 2101 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa 2161 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat 2221 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct 2281 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat 2341 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt 2401 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 2461 gaatgcaatt gttgttgttg ggctgcagga attaattcga gctcgcccga cattgattat 2521 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 2581 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 2641 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 2701 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 2761 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 2821 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 2881 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 2941 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 3001 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 3061 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga 3121 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg 3181 gccgcgttga cgcgcacggc aagaggcgag gggcggcgac tggtgagaga tgggtgcgag 3241 agcgtcagta ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg 3301 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt 3361 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct 3421 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac 3481 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat 3541 agaggaagag caaaacaaaa gtaagaaaaa agcacagcaa gcagcagctg acacaggaca 3601 cagcaatcag gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca 3661 tcaggccata tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt 3721 cagcccagaa gtgataccca tgttttcagc attatcagaa ggagccaccc cacaagattt 3781 aaacaccatg ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac 3841 catcaatgag gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattgc 3901 accaggccag atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca 3961 ggaacaaata ggatggatga cacataatcc acctatccca gtaggagaaa tctataaaag 4021 atggataatc ctgggattaa ataaaatagt aagaatgtat agccctacca gcattctgga 4081 cataagacaa ggaccaaagg aaccctttag agactatgta gaccgattct ataaaactct 4141 aagagccgag caagcttcac aagaggtaaa aaattggatg acagaaacct tgttggtcca 4201 aaatgcgaac ccagattgta agactatttt aaaagcattg ggaccaggag cgacactaga 4261 agaaatgatg acagcatgtc agggagtggg gggacccggc cataaagcaa gagttttggc 4321 tgaagcaatg agccaagtaa caaatccagc taccataatg atacagaaag gcaattttag 4381 gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa gaagggcaca tagccaaaaa 4441 ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga aaggaaggac accaaatgaa 4501 agattgtact gagagacagg ctaatttttt agggaagatc tggccttccc acaagggaag 4561 gccagggaat tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag 4621 gtttggggaa gagacaacaa ctccctctca gaagcaggag ccgatagaca aggaactgta 4681 tcctttagct tccctcagat cactctttgg cagcgacccc tcgtcacaat aaagataggg 4741 gggcaattaa aggaagctct attagataca ggagcagatg atacagtatt agaagaaatg 4801 aatttgccag gaagatggaa accaaaaatg atagggggaa ttggaggttt tatcaaagta 4861 agacagtatg atcagatact catagaaatc tgcggacata aagctatagg tacagtatta 4921 gtaggaccta cacctgtcaa cataattgga agaaatctgt tgactcagat tggctgcact 4981 ttaaattttc ccattagtcc tattgagact gtaccagtaa aattaaagcc aggaatggat 5041 ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa taaaagcatt agtagaaatt 5101 tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg ggcctgaaaa tccatacaat 5161 actccagtat ttgccataaa gaaaaaagac agtactaaat ggagaaaatt agtagatttc 5221 agagaactta ataagagaac tcaagatttc tgggaagttc aattaggaat accacatcct 5281 gcagggttaa aacagaaaaa atcagtaaca gtactggatg tgggcgatgc atatttttca 5341 gttcccttag ataaagactt caggaagtat actgcattta ccatacctag tataaacaat 5401 gagacaccag ggattagata tcagtacaat gtgcttccac agggatggaa aggatcacca 5461 gcaatattcc agtgtagcat gacaaaaatc ttagagcctt ttagaaaaca aaatccagac 5521 atagtcatct atcaatacat ggatgatttg tatgtaggat ctgacttaga aatagggcag 5581 catagaacaa aaatagagga actgagacaa catctgttga ggtggggatt taccacacca 5641 gacaaaaaac atcagaaaga acctccattc ctttggatgg gttatgaact ccatcctgat 5701 aaatggacag tacagcctat agtgctgcca gaaaaggaca gctggactgt caatgacata 5761 cagaaattag tgggaaaatt gaattgggca agtcagattt atgcagggat taaagtaagg 5821 caattatgta aacttcttag gggaaccaaa gcactaacag aagtagtacc actaacagaa 5881 gaagcagagc tagaactggc agaaaacagg gagattctaa aagaaccggt acatggagtg 5941 tattatgacc catcaaaaga cttaatagca gaaatacaga agcaggggca aggccaatgg 6001 acatatcaaa tttatcaaga gccatttaaa aatctgaaaa caggaaagta tgcaagaatg 6061 aagggtgccc acactaatga tgtgaaacaa ttaacagagg cagtacaaaa aatagccaca 6121 gaaagcatag taatatgggg aaagactcct aaatttaaat tacccataca aaaggaaaca 6181 tgggaagcat ggtggacaga gtattggcaa gccacctgga ttcctgagtg ggagtttgtc 6241 aatacccctc ccttagtgaa gttatggtac cagttagaga aagaacccat aataggagca 6301 gaaactttct atgtagatgg ggcagccaat agggaaacta aattaggaaa agcaggatat 6361 gtaactgaca gaggaagaca aaaagttgtc cccctaacgg acacaacaaa tcagaagact 6421 gagttacaag caattcatct agctttgcag gattcgggat tagaagtaaa catagtgaca 6481 gactcacaat atgcattggg aatcattcaa gcacaaccag ataagagtga atcagagtta 6541 gtcagtcaaa taatagagca gttaataaaa aaggaaaaag tctacctggc atgggtacca 6601 gcacacaaag gaattggagg aaatgaacaa gtagataaat tggtcagtgc tggaatcagg 6661 aaagtactat ttttagatgg aatagataag gcccaagaag aacatgagaa atatcacagt 6721 aattggagag caatggctag tgattttaac ctaccacctg tagtagcaaa agaaatagta 6781 gccagctgtg ataaatgtca gctaaaaggg gaagccatgc atggacaagt agactgtagc 6841 ccaggaatat ggcagctaga ttgtacacat ttagaaggaa aagttatctt ggtagcagtt 6901 catgtagcca gtggatatat agaagcagaa gtaattccag cagagacagg gcaagaaaca 6961 gcatacttcc tcttaaaatt agcaggaaga tggccagtaa aaacagtaca tacagacaat 7021 ggcagcaatt tcaccagtac tacagttaag gccgcctgtt ggtgggcggg gatcaagcag 7081 gaatttggca ttccctacaa tccccaaagt caaggagtaa tagaatctat gaataaagaa 7141 ttaaagaaaa ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa 7201 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg 7261 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 7321 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggaaagga 7381 ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata 7441 aaagtagtgc caagaagaaa agcaaagatc atcagggatt atggaaaaca gatggcaggt 7501 gatgattgtg tggcaagtag acaggatgag gattaacaca tggaattctg caacaactgc 7561 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga 7621 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag 7681 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt 7741 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga 7801 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta 7861 atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt 7921 gtgtggtcca tagtaatcat agaatatagg aaaatggccg ctgatcttca gacctggagg 7981 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga 8041 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 8101 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 8161 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 8221 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 8281 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 8341 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 8401 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 8461 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 8521 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 8581 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 8641 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 8701 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 8761 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 8821 cttggcactt atctgggacg atctgcggag cctgtgcctc ttcagctacc accgcttgag 8881 agacttactc ttgattgtaa cgaggattgt ggaacttctg ggacgcaggg ggtgggaagc 8941 cctcaaatat tggtggaatc tcctacaata ttggagtcag gagctaaaga atagtgctgt 9001 tagcttgctc aatgccacag ccatagcagt agctgagggg acagataggg ttatagaagt 9061 agtacaagga gcttgtagag ctattcgcca catacctaga agaataagac agggcttgga 9121 aaggattttg ctataagctc gagtgacctt cagaccttgg cactggaggt ggcccggcag 9181 aagcgcggca tcgtggatca gtgctgcacc agcatctgct ctctctacca actggagaac 9241 tactgcaact aggcccacca ctaccctgtc cacccctctg caatgaataa aacctttgaa 9301 agagcactac aagttgtgtg tacatgcgtg catgtgcata tgtggtgcgg ggggaacatg 9361 agtggggctg gctggagtgg cgatgataag ctgtcaaaca tgagaattaa ttcttgaaga 9421 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 9481 tagtctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca 9541 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt 9601 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg 9661 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag 9721 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg 9781 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga 9841 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg 9901 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc 9961 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg 10021 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 10081 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 10141 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 10201 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 10261 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 10321 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 10381 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 10441 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 10501 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 10561 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 10621 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 10681 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 10741 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 10801 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 10861 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 10921 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 10981 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 11041 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 11101 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 11161 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 11221 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 11281 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 11341 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 11401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 11461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 11521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 11581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 11641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 11701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 11761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 11821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 11881 ggtatccggt aagcggcagg gtcg //