LOCUS Exported 9481 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of CSNK1A1. ACCESSION . VERSION . KEYWORDS CSNK1A1_pLX307 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9481) AUTHORS Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe J, Lage K, Boehm JS, Hahn WC TITLE Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in beta-Catenin-Active Cancers. JOURNAL Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 10.1016/j.cels.2016.09.001. PUBMED 27684187 REFERENCE 2 (bases 1 to 9481) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9481 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 98..1276 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 329..1267 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1224..1244 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1296..1320 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2332..2356) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2364..2405 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2516..3104 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2569..2589) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2987..2998) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3176..3409 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3487..3608 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3524..3543) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3578..3597 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 3648..3783 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(3803..3822) /label=T7 /note="T7 promoter, forward primer" promoter complement(3804..3822) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3832..3849) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3832..3848) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3841..3863) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3990..4445 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4077..4096) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4287..4308 /label=F1ori-F /note="F1 origin, forward primer" promoter 4471..4575 /gene="bla" /label=AmpR promoter CDS 4576..5436 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4794..4813) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5607..6195 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6096..6115 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6349..6366 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6483..6504 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6519..6549 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6557..6573 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6562..6584 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6581..6597 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6581..6597 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6616..6636 /label=T3 /note="T3 promoter, forward primer" promoter 6618..6636 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 6664..6890 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 6891..7071 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7118..7243 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7736..7969 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8154..8198 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 8429..8639 /label=SV40 promoter /note="SV40 early promoter" promoter 8451..8647 /label=SV40 promoter /note="SV40 early promoter" rep_origin 8498..8633 /label=SV40 ori /note="SV40 origin of replication" CDS 8679..9281 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(8679..8698) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9175..9195 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 9324..9441 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag 61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc 121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt 421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca 1321 tggcgagtag cagcggctcc aaggctgaat tcattgtcgg agggaaatat aaactggtac 1381 ggaagatcgg gtctggctcc ttcggggaca tctatttggc gatcaacatc accaacggcg 1441 aggaagtggc agtgaagcta gaatctcaga aggccaggca tccccagttg ctgtacgaga 1501 gcaagctcta taagattctt caaggtgggg ttggcatccc ccacatacgg tggtatggtc 1561 aggaaaaaga ctacaatgta ctagtcatgg atcttctggg acctagcctc gaagacctct 1621 tcaatttctg ttcaagaagg ttcacaatga aaactgtact tatgttagct gaccagatga 1681 tcagtagaat tgaatatgtg catacaaaga attttataca cagagacatt aaaccagata 1741 acttcctaat gggtattggg cgtcactgta ataagttatt ccttattgat tttggtttgg 1801 ccaaaaagta cagagacaac aggacaaggc aacacatacc atacagagaa gataaaaacc 1861 tcactggcac tgcccgatat gctagcatca atgcacatct tggtattgag cagagtcgcc 1921 gagatgacat ggaatcatta ggatatgttt tgatgtattt taatagaacc agcctgccat 1981 ggcaagggct aaaggctgca acaaagaaaa aaaaatatga aaagattagt gaaaagaaga 2041 tgtccacgcc tgttgaagtt ttatgtaagg ggtttcctgc agaatttgcg atgtacttaa 2101 actattgtcg tgggctacgc tttgaggaag ccccagatta catgtatctg aggcagctat 2161 tccgcattct tttcaggacc ctgaaccatc aatatgacta cacatttgat tggacaatgt 2221 taaagcagaa agcagcacag caggcagcct cttccagtgg gcagggtcag caggcccaaa 2281 cccccacagg caagcaaact gacaaaacca agagtaacat gaaaggtttc tacccaactt 2341 tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc ggtctcgatt 2401 ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat 2461 cttgtggaaa ggacgaaatt tccattagtc ttccttataa acgcgttaag tcgacaatca 2521 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 2581 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 2641 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 2701 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 2761 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 2821 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 2881 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 2941 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 3001 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 3061 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca 3121 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 3181 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 3241 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 3301 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 3361 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt 3421 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 3481 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 3541 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 3601 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa 3661 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 3721 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 3781 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 3841 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 3901 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 3961 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 4021 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 4081 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 4141 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 4201 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 4261 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 4321 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 4381 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 4441 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 4501 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 4561 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 4621 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 4681 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 4741 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 4801 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 4861 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 4921 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 4981 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 5041 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 5101 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 5161 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 5221 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 5281 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 5341 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 5401 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 5461 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 5521 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 5581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 5641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 5701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 5761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 5821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 5881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 5941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 6001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 6061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 6121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 6181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 6241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 6301 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 6361 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 6421 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 6481 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 6541 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 6601 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct 6661 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 6721 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 6781 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat 6841 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct 6901 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 6961 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 7021 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc 7081 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct 7141 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 7201 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 7261 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 7321 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 7381 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 7441 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 7501 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 7561 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg 7621 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 7681 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 7741 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 7801 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 7861 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 7921 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 7981 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 8041 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 8101 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 8161 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 8221 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 8281 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 8341 ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac 8401 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa 8461 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 8521 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 8581 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 8641 ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg 8701 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 8761 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 8821 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 8881 acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 8941 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 9001 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 9061 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 9121 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 9181 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 9241 cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga 9301 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca 9361 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa 9421 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc 9481 c //