LOCUS       Exported                9640 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral expression of TGFB1.
ACCESSION   .
VERSION     .
KEYWORDS    TGFB1_pLX307
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2491..2515)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2523..2564
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2675..3263
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2728..2748)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3146..3157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3335..3568
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3646..3767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3683..3702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3737..3756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3807..3942
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3962..3981)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3963..3981)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3991..4008)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3991..4007)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4000..4022)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4149..4604
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4236..4255)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4446..4467
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4630..4734
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4735..5595
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4953..4972)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5766..6354
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6255..6274
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6508..6525
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6642..6663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6678..6708
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6716..6732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6721..6743
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6740..6756
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6740..6756
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6775..6795
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6777..6795
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6823..7049
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7050..7230
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7277..7402
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7895..8128
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8313..8357
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8588..8798
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8610..8806
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8657..8792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8838..9440
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8838..8857)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9334..9354
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9483..9600
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgccgccctc cgggctgcgg ctgctgctgc tgctgctacc gctgctgtgg ctactggtgc
     1381 tgacgcctgg ccggccggcc gcgggactat ccacctgcaa gactatcgac atggagctgg
     1441 tgaagcggaa gcgcatcgag gccatccgcg gccagatcct gtccaagctg cggctcgcca
     1501 gccccccgag ccagggggag gtgccgcccg gcccgctgcc cgaggccgtg ctcgccctgt
     1561 acaacagcac ccgcgaccgg gtggccgggg agagtgcaga accggagccc gagcctgagg
     1621 ccgactacta cgccaaggag gtcacccgcg tgctaatggt ggaaacccac aacgaaatct
     1681 atgacaagtt caagcagagt acacacagca tatatatgtt cttcaacaca tcagagctcc
     1741 gagaagcggt acctgaaccc gtgttgctct cccgggcaga gctgcgtctg ctgaggctca
     1801 agttaaaagt ggagcagcac gtggagctgt accagaaata cagcaacaat tcctggcgat
     1861 acctcagcaa ccggctgctg gcacccagcg actcgccaga gtggttatct tttgatgtca
     1921 ccggagttgt gcggcagtgg ttgagccgtg gaggggaaat tgagggcttt cgccttagcg
     1981 cccactgctc ctgtgacagc agggataaca cactgcaagt ggacatcaac gggttcacta
     2041 ccggccgccg aggtgacctg gccaccattc atggcatgaa ccggcctttc ctgcttctca
     2101 tggccacccc gctggagagg gcccagcatc tgcaaagctc ccggcaccgc cgagccctgg
     2161 acaccaacta ttgcttcagc tccacggaga agaactgctg cgtgcggcag ctgtacattg
     2221 acttccgcaa ggacctcggc tggaagtgga tccacgagcc caagggctac catgccaact
     2281 tctgcctcgg gccctgcccc tacatttgga gcctggacac gcagtacagc aaggtcctgg
     2341 ccctgtacaa ccagcataac ccgggcgcct cggcggcgcc gtgctgcgtg ccgcaggcgc
     2401 tggagccgct gcccatcgtg tactacgtgg gccgcaagcc caaggtggag cagctgtcca
     2461 acatgatcgt gcgctcctgc aagtgcagct gcccaacttt cttgtacaaa gtggttgata
     2521 tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgaactagtc
     2581 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgatttac
     2641 cgtttctgag attactgcca cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     2701 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2761 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2821 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2881 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2941 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3001 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3061 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     3121 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3181 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3241 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     3301 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     3361 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     3421 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3481 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3541 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     3601 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     3661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     3781 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3841 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3901 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     3961 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     4021 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4081 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4141 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4201 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4441 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4501 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4561 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     4621 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4681 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4741 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4801 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4861 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4921 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4981 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5041 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5101 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5161 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5221 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5281 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5341 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5401 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5461 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5521 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5581 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5641 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5701 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5761 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5821 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5881 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5941 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6001 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6061 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6121 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6181 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6241 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6301 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6361 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6421 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6481 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6541 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6601 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     6661 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6721 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     6781 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     6841 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     6901 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     6961 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     7021 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     7081 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7141 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7201 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     7261 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     7321 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     7381 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     7441 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     7501 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     7561 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     7621 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     7681 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     7741 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     7801 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     7861 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     7921 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     7981 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     8041 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     8101 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     8161 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     8221 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     8281 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     8341 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     8401 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     8461 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     8521 cgaggggacc catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8581 ctccccagca ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc
     8641 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8701 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8761 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg
     8821 taccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     8881 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     8941 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     9001 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg
     9061 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     9121 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     9181 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     9241 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     9301 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     9361 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     9421 gcaagcccgg tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac
     9481 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     9541 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     9601 cgggtttatt acagggacag cagagatcca ctttggcgcc
//
