LOCUS Exported 9838 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of MYC. ACCESSION . VERSION . KEYWORDS MYC_pLX307 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9838) AUTHORS Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe J, Lage K, Boehm JS, Hahn WC TITLE Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in beta-Catenin-Active Cancers. JOURNAL Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 10.1016/j.cels.2016.09.001. PUBMED 27684187 REFERENCE 2 (bases 1 to 9838) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9838 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 98..1276 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 329..1267 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1224..1244 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1296..1320 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1368..2687 /codon_start=1 /product="human c-Myc proto-oncogene" /label=c-Myc /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA" protein_bind complement(2689..2713) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2721..2762 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2873..3461 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2926..2946) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3344..3355) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3533..3766 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3844..3965 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3881..3900) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3935..3954 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 4005..4140 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(4160..4179) /label=T7 /note="T7 promoter, forward primer" promoter complement(4161..4179) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4189..4206) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4189..4205) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4198..4220) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4347..4802 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4434..4453) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4644..4665 /label=F1ori-F /note="F1 origin, forward primer" promoter 4828..4932 /gene="bla" /label=AmpR promoter CDS 4933..5793 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5151..5170) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5964..6552 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6453..6472 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6706..6723 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6840..6861 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6876..6906 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6914..6930 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6919..6941 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6938..6954 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6938..6954 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6973..6993 /label=T3 /note="T3 promoter, forward primer" promoter 6975..6993 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 7021..7247 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7248..7428 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7475..7600 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8093..8326 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8511..8555 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 8786..8996 /label=SV40 promoter /note="SV40 early promoter" promoter 8808..9004 /label=SV40 promoter /note="SV40 early promoter" rep_origin 8855..8990 /label=SV40 ori /note="SV40 origin of replication" CDS 9036..9638 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(9036..9055) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9532..9552 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 9681..9798 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag 61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc 121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg 361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt 421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca 1321 ccatggattt ttttcgggta gtggaaaacc agcagcctcc cgcgacgatg cccctcaacg 1381 ttagcttcac caacaggaac tatgacctcg actacgactc ggtgcagccg tatttctact 1441 gcgacgagga ggagaacttc taccagcagc agcagcagag cgagctgcag cccccggcgc 1501 ccagcgagga tatctggaag aaattcgagc tgctgcccac cccgcccctg tcccctagcc 1561 gccgctccgg gctctgctcg ccctcctacg ttgcggtcac acccttctcc cttcggggag 1621 acaacgacgg cggtggcggg agcttctcca cggccgacca gctggagatg gtgaccgagc 1681 tgctgggagg agacatggtg aaccagagtt tcatctgcga cccggacgac gagaccttca 1741 tcaaaaacat catcatccag gactgtatgt ggagcggctt ctcggccgcc gccaagctcg 1801 tctcagagaa gctggcctcc taccaggctg cgcgcaaaga cagcggcagc ccgaaccccg 1861 cccgcggcca cagcgtctgc tccacctcca gcttgtacct gcaggatctg agcgccgccg 1921 cctcagagtg catcgacccc tcggtggtct tcccctaccc tctcaacgac agcagctcgc 1981 ccaagtcctg cgcctcgcaa gactccagcg ccttctctcc gtcctcggat tctctgctct 2041 cctcgacgga gtcctccccg cagggcagcc ccgagcccct ggtgctccat gaggagacac 2101 cgcccaccac cagcagcgac tctgaggagg aacaagaaga tgaggaagaa atcgatgttg 2161 tttctgtgga aaagaggcag gctcctggca aaaggtcaga gtctggatca ccttctgctg 2221 gaggccacag caaacctcct cacagcccac tggtcctcaa gaggtgccac gtctccacac 2281 atcagcacaa ctacgcagcg cctccctcca ctcggaagga ctatcctgct gccaagaggg 2341 tcaagttgga cagtgtcaga gtcctgagac agatcagcaa caaccgaaaa tgcaccagcc 2401 ccaggtcctc ggacaccgag gagaatgtca agaggcgaac acacaacgtc ttggagcgcc 2461 agaggaggaa cgagctaaaa cggagctttt ttgccctgcg tgaccagatc ccggagttgg 2521 aaaacaatga aaaggccccc aaggtagtta tccttaaaaa agccacagca tacatcctgt 2581 ccgtccaagc agaggagcaa aagctcattt ctgaagagga cttgttgcgg aaacgacgag 2641 aacagttgaa acacaaactt gaacagctac ggaactcttg tgcgtaattg ccaactttct 2701 tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2761 cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt 2821 gtggaaagga cgaagacacg ggtcgggcgt ctcaagcacg cgttaagtcg acaatcaacc 2881 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 2941 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 3001 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 3061 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 3121 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 3181 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 3241 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 3301 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 3361 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 3421 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg 3481 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg 3541 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta 3601 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 3661 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 3721 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt 3781 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 3841 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3901 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3961 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc 4021 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 4081 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 4141 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt 4201 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 4261 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 4321 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg 4381 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 4441 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 4501 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 4561 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 4621 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 4681 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 4741 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 4801 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 4861 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 4921 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 4981 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 5041 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 5101 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 5161 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 5221 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 5281 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 5341 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 5401 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 5461 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 5521 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 5581 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 5641 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 5701 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 5761 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 5821 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 5881 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 5941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 6001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 6061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 6121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 6181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 6241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 6301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 6361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 6421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 6481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 6541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 6601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 6661 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 6721 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 6781 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 6841 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 6901 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat 6961 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa 7021 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg 7081 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc 7141 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc 7201 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt 7261 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 7321 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 7381 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 7441 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 7501 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 7561 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 7621 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 7681 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 7741 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 7801 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 7861 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 7921 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca 7981 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac 8041 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt 8101 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga 8161 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg 8221 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg 8281 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt 8341 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat 8401 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt 8461 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac 8521 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt 8581 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag 8641 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc 8701 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag 8761 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta 8821 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 8881 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 8941 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 9001 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc 9061 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 9121 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 9181 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 9241 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 9301 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 9361 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 9421 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 9481 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 9541 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 9601 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa 9661 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg 9721 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 9781 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgcc //