LOCUS Exported 6482 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS CD44S pBabe-puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6482) AUTHORS Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA TITLE Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. JOURNAL Cell. 2008 Jul 11. 134(1):62-73. PUBMED 18614011 REFERENCE 2 (bases 1 to 6482) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6482 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(594..1454) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1217..1236 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1455..1559) /gene="bla" /label=AmpR promoter LTR 1590..2059 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 2124..2323 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 2524..2940 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 2870..2892 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 2908..2924 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind complement(4394..4414) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4399..4728 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4579..4714 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4641..4660 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4738..5337 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4738..4757) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5234..5254 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 5558..6027 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(6157..6174) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6328..434) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6408..6427) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 61 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 121 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 181 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 241 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 301 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 361 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 421 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 481 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 541 ttttgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 661 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 901 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1141 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1201 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1261 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1321 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1381 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1441 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1501 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 1561 acatttcccc gaaaagtgcc acctgcagcc tgaatatggg ccaaacagga tatctgtggt 1621 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa 1681 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca 1741 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa 1801 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg 1861 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc 1921 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt 1981 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc 2041 cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga 2101 ccaccgaccc accaccggga ggcaagctgg ccagcaactt atctgtgtct gtccgattgt 2161 ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa ctagctctgt 2221 atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga 2281 cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg 2341 aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg 2401 cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag 2461 cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat 2521 tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag 2581 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct 2641 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc 2701 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc 2761 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt 2821 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct 2881 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc 2941 ggccggatcc cagtgtggtg gtacgtagga attcgccagc acagtggtcg agatccattg 3001 tgctctaaag ccagcctctg ccaggttcgg tccgccatcc tcgtcccgtc ctccgccggc 3061 ccctgccccg cgcccaggga tcctccagct cctttcgccc gcgccctccg ttcgctccgg 3121 acaccatgga caagttttgg tggcacgcag cctggggact ctgcctcgtg ccgctgagcc 3181 tggcgcagat cgatttgaat ataacctgcc gctttgcagg tgtattccac gtggagaaaa 3241 atggtcgcta cagcatctct cggacggagg ccgctgacct ctgcaaggct ttcaatagca 3301 ccttgcccac aatggcccag atggagaaag ctctgagcat cggatttgag acctgcaggt 3361 atgggttcat agaagggcat gtggtgattc cccggatcca ccccaactcc atctgtgcag 3421 caaacaacac aggggtgtac atcctcacat acaacacctc ccagtatgac acatattgct 3481 tcaatgcttc agctccacct gaagaagatt gtacatcagt cacagacctg cccaatgcct 3541 ttgatggacc aattaccata actattgtta accgtgatgg cacccgctat gtccagaaag 3601 gagaatacag aacgaatcct gaagacatct accccagcaa ccctactgat gatgacgtga 3661 gcagcggctc ctccagtgaa aggagcagca cttcaggagg ttacatcttt tacacctttt 3721 ctactgtaca ccccatccca gacgaagaca gtccctggat caccgacagc acagacagaa 3781 tccctgctac cagagaccaa gacacattcc accccagtgg ggggtcccat accactcatg 3841 aatctgaatc agatggacac tcacatggga gtcaagaagg tggagcaaac acaacctctg 3901 gtcctataag gacaccccaa attccagaat ggctgatcat cttggcatcc ctcttggcct 3961 tggctttgat tcttgcagtt tgcattgcag tcaacagtcg aagaaggtgt gggcagaaga 4021 aaaagctagt gatcaacagt ggcaatggag ctgtggagga cagaaagcca agtggactca 4081 acggagaggc cagcaagtct caggaaatgg tgcatttggt gaacaaggag tcgtcagaaa 4141 ctccagacca gtttatgaca gctgatgaga caaggaacct gcagaatgtg gacatgaaga 4201 ttggggtgta acacctacac cattatcttg gaaagaaaca accgttgtaa acataaccat 4261 tacagggagc tgggacactt aacagatgca atgtgctact gattgtttca ttgcgaatct 4321 tttttagcat aaaattttct actctttttg ttaaaaaaaa aaaactttag agcacaatgg 4381 atctcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 4441 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 4501 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 4561 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 4621 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 4681 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaccatg 4741 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 4801 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 4861 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 4921 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 4981 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5041 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5101 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5161 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5221 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5281 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc 5341 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgataaaa 5401 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt 5461 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata 5521 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata 5581 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat 5641 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat 5701 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg 5761 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct 5821 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact 5881 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct 5941 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 6001 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt 6061 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg 6121 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 6181 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 6241 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6301 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6361 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6421 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6481 cc //