LOCUS Exported 9621 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF. ACCESSION . VERSION . KEYWORDS pWZL-Neo-Myr-Flag-DEST SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9621) AUTHORS Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC TITLE Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene (Kinase Library Clones) JOURNAL Cell. 2007 Jun 15. 129(6):1065-1079. PUBMED 17574021 REFERENCE 2 (bases 1 to 9621) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9621 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 149..528 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 529..732 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 683..703 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 953..1152 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1353..1769 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1699..1721 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1737..1753 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" regulatory 1787..1796 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1793..1834 /codon_start=1 /product="N-myristoylation signal from Src kinase (Pellman et al., 1985; Kaplan et al., 1988)" /label=myr /translation="MGSSKSKPKDPSQR" CDS 1859..1882 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" protein_bind 1889..2013 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 2038..2068 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 2122..2781 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(2188..2207) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 3123..3428 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 3307..3326 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(3469..3593) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" misc_feature 3652..4113 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3708..3725) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3935..3954 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4118..4912 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4172..4191) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4782..4801 /label=Neo-F /note="Neomycin resistance gene, forward primer" LTR 5099..5568 /note="long terminal repeat from Moloney murine leukemia virus" promoter 5604..5708 /gene="bla" /label=AmpR promoter CDS 5709..6569 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5927..5946) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8335..8923 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8824..8843 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9077..9094 /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9198..4) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9308..9329) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 9520..9539 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 cgcgccgatc ttcaatattg gccattagcc atattattca ttggttatat agcataaatc 61 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt 121 ggctcatgtc caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa 181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt 421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac 481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 541 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 661 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 721 ataagcagag ctggtttagt gaaccgcgcc agtcctccga ttgactgagt cgcccgggta 781 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg 841 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg 901 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gcaagctggc 961 cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc 1021 gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt 1081 tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg 1141 gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg 1201 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga 1261 accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc 1321 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt 1381 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca 1441 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc 1501 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac 1561 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt 1621 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc 1681 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt 1741 atccagccct cactccttct ctaggcgccg gccggatcca gaattcgcca ccatggggtc 1801 ttcaaaatct aaaccaaagg accccagcca gcgccggcgc aggatccgag gttaccttga 1861 ctacaaagac gatgacgaca agcaattgac aagtttgtac aaaaaagctg aacgagaaac 1921 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata 1981 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt 2041 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt 2101 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata 2161 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat 2221 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac 2281 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc 2341 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc 2401 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc 2461 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat 2521 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc 2581 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg 2641 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat 2701 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 2761 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat 2821 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt 2881 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct 2941 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca 3001 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc 3061 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg 3121 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg 3181 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac 3241 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa 3301 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag 3361 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg 3421 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg 3481 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata 3541 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtttgatct 3601 agtcgaccaa ttccggttat tttccaccat attgccgtct tttggcaatg tgagggcccg 3661 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg 3721 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca 3781 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct 3841 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca 3901 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa 3961 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg 4021 cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg 4081 gacgtggttt tcctttgaaa aacacgatga taataccatg attgaacaag atggattgca 4141 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 4201 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 4261 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 4321 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 4381 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 4441 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 4501 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 4561 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 4621 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 4681 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 4741 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 4801 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 4861 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 4921 ctggggttcg catcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg 4981 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg 5041 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct 5101 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 5161 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc 5221 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag 5281 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa 5341 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa 5401 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta 5461 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg 5521 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca ggccagtggg 5581 gccgcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 5641 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 5701 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 5761 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 5821 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 5881 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 5941 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 6001 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 6061 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 6121 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 6181 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 6241 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 6301 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 6361 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 6421 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 6481 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 6541 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 6601 agattgattt gcggccgcga aaaggacaag cagcgaaaat tcacgccccc ttgggaggtg 6661 gcggcatatg caaaggatag cactcccact ctactactgg gtatcatatg ctgactgtat 6721 atgcatgagg atagcatatg ctacccggat acagattagg atagcatata ctacccagat 6781 atagattagg atagcatatg ctacccagat atagattagg atagcctatg ctacccagat 6841 ataaattagg atagcatata ctacccagat atagattagg atagcatatg ctacccagat 6901 atagattagg atagcctatg ctacccagat atagattagg atagcatatg ctatccagat 6961 atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca gcgacctcgt 7021 gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc 7081 tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg caggtattcc 7141 ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta 7201 caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc ggcgtgtggg 7261 ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt gtctttgttt 7321 atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga caaccagtgg 7381 agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt gtaacaacat 7441 ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta tcatattgca 7501 ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc agtctttacg 7561 gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt cattcctaca 7621 ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc acaatgccac 7681 cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt gttttcgagc 7741 acctcacata caccttactg ttcacaactc agcagttatt ctattagcta aacgaaggag 7801 aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat cttcagccac 7861 tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa 7921 ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt actaacccta 7981 attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg ttagtctgga 8041 tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca agggggcctt 8101 ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac acaggcccct 8161 ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg tacatgtcgg 8221 ccgcaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 8281 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 8341 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 8401 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 8461 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 8521 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 8581 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 8641 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 8701 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 8761 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 8821 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 8881 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 8941 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 9001 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 9061 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 9121 accgcctctc cccgcgcgtt ggccgattca ttaatgcaac tatggttggc gcgttgtaaa 9181 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca 9241 atagaccgaa atcggcaaaa tcccttataa atcaaaagaa tagcccgaga tagagttgag 9301 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 9361 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt 9421 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 9481 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 9541 gggcgctaag gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 9601 gcttaatgcg ccgctacagg g //