LOCUS Exported 3415 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning. ACCESSION . VERSION . KEYWORDS p5E-Runx1+23 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3415) AUTHORS Tamplin OJ, Durand EM, Carr LA, Childs SJ, Hagedorn EJ, Li P, Yzaguirre AD, Speck NA, Zon LI TITLE Hematopoietic stem cell arrival triggers dynamic remodeling of the perivascular niche. JOURNAL Cell. 2015 Jan 15;160(1-2):241-52. doi: 10.1016/j.cell.2014.12.032. PUBMED 25594182 REFERENCE 2 (bases 1 to 3415) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3415 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 88..105 /label=L4440 /note="L4440 vector, forward primer" terminator 264..291 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 383..469 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 518..540 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 532..549 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 533..549 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 589..684 /gene="mutant version of attL" /label=attL4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 686..704 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(1438..1456) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" protein_bind 1456..1580 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter complement(1653..1671) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1676..1692) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1676..1692) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1794..1813) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1805..2614 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1872..1891) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2761..3349 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3250..3269 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 61 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 121 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 181 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc 241 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt 301 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa 361 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac 421 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag 481 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg 541 acggccagtc ttaagctcgg gcccgagtta acgctaccat ggagctccaa ataatgattt 601 tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc ttttttataa 661 tgccaacttt gtatagaaaa gttggtaata cgactcacta tagggcgaat tgggtaccgg 721 gccccccctc gagggatccg gggtgggagg tgtaagttcc acccccaccc ttcctgacac 781 gctcctgaac cctggccact gcacctgcta ggttctcact tctctgggaa gcatctagaa 841 acaggacctc tcacccaccc ctcccggctg ggctctaggg tggggccctc actacctctt 901 ttcttctcaa agagcctggg atgctgacag cctcagatgg aggcatcctg tttgtcgaaa 961 aataaaccgg cagttgaagc cgggttgcaa gagcgagaaa accgcaggcc tgcgcgccac 1021 tgataacgtg ggcagcttgc ttttgcagca gttcctagct gcagcggccc tgtgaaggcc 1081 tgtgtcaccg cctcccttcc tgtctcctcc tcacaccatc cctccatcgc tccttgctgg 1141 ctctaccagc cacttgctgg acccttcagc cactgggacc attgctttcc ataaaaaact 1201 ccttagtgca gtgcagagct gatcagaggg tagcagggaa ccctgacgcc tgatgggttg 1261 ctgacacctg agatccacta gtccaatctg ctcagagagg acagagtggg caggagccag 1321 cattgggtat ataaagctga gcagggtcag ttgcttctta cgtttgcttc tgagtctgtt 1381 gtgttgactt gcaacctcag aaacagacat cccgcggtgg agctccagct tttgttccct 1441 ttagtgaggg ttaattcaag tttgtacaaa aaagttgaac gagaaacgta aaatgatata 1501 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca 1561 caacatatgc agtcactatg aaccaactac ttagatggta ttagtgacct gtagaattcg 1621 agctctagag ctgcagggcg gccgcgatat cccctatagt gagtcgtatt acatggtcat 1681 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga 1741 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg 1801 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc 1861 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta 1921 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt 1981 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct 2041 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat 2101 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt 2161 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt 2221 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt 2281 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga 2341 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact 2401 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg 2461 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc 2521 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt 2581 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca 2641 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa 2701 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 2761 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 2821 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 2881 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 2941 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 3001 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3061 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 3121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 3181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 3241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 3301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 3361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tcttt //