LOCUS Exported 4733 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: N1 Cloning Vector, Excitation: 433, Emission: 475. ACCESSION . VERSION . KEYWORDS mCerulean3-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4733) AUTHORS Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA TITLE An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching. JOURNAL PLoS One. 2011 . 6(3):e17896. PUBMED 21479270 REFERENCE 2 (bases 1 to 4733) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4733 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 531..611 /label=MCS /note="multiple cloning site" regulatory 613..622 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(664..685) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(925..944) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1272..1293 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 1461..1582 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1498..1517) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1552..1571 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1589..2044) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1726..1747) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1938..1957 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2071..2175 /gene="bla" /label=AmpR promoter promoter 2177..2534 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2200..2220) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2385..2520 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2447..2466 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2569..3363 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2623..2642) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3233..3252 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3551..3570) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3595..3642 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3971..4559 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4460..4479 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 601 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 661 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 721 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 781 cccgtgccct ggcccaccct cgtgaccacc ctgagctggg gcgtgcagtg cttcgcccgc 841 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 901 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 961 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 1021 ggcaacatcc tggggcacaa gctggagtac aacgccatcc acggcaacgt ctatatcacc 1081 gccgacaagc agaagaacgg catcaaggcc aacttcggcc tcaactgcaa catcgaggac 1141 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 1201 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag 1261 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 1321 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg 1381 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 1441 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 1501 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 1561 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc 1621 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc 1681 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag 1741 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 1801 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa 1861 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 1921 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 1981 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc 2041 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 2101 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 2161 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt 2221 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2281 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2341 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 2401 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 2461 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 2521 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa 2581 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg 2641 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc 2701 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca 2761 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc 2821 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca 2881 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat 2941 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca 3001 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg 3061 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc 3121 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct 3181 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct 3241 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac 3301 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc 3361 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 3421 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 3481 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg 3541 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa 3601 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt 3661 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 3721 tttcttcctt ttccccaccc catcccccaa gttcgggtga aggcccaggg ctcgcagcca 3781 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt 3841 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 3901 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 3961 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 4021 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 4081 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 4141 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 4201 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 4261 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 4321 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 4381 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 4441 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 4501 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 4561 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 4621 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat 4681 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccg //