LOCUS Exported 11303 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Cry2olig(E490G). ACCESSION . VERSION . KEYWORDS pHR-mCh-Cry2olig SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11303) AUTHORS Shin Y, Berry J, Pannucci N, Haataja MP, Toettcher JE, Brangwynne CP TITLE Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets. JOURNAL Cell. 2017 Jan 12;168(1-2):159-171.e14. doi: 10.1016/j.cell.2016.11.054. Epub 2016 Dec 29. PUBMED 28041848 REFERENCE 2 (bases 1 to 11303) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11303 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 43..160 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 313..720 /label=SFFV promoter /note="spleen focus-forming virus long terminal repeat (LTR) promoter" CDS 1515..3008 /codon_start=1 /product="N-terminal photolyase homology region of Arabidopsis thaliana cryptochrome 2" /label=CRY2PHR /note="binds to CIB1 upon exposure to blue light (Kennedy et al., 2010)" /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA" misc_feature 3160..3748 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3213..3233) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3631..3642) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(3751..3767) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3752..3768) /label=pBluescriptKS /note="For pBluescript vector" LTR 3858..4091 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter complement(4116..4134) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4117..4134) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4424..4443) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(4657..4676) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4776..4798 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4836..4854) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4922..5026 /gene="bla" /label=AmpR promoter CDS 5027..5887 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5245..5264) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6058..6646 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6547..6566 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6800..6817 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(6887..6907) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6892..7221 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7072..7207 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7134..7153 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 8355..8420 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 8550..8570 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 8995..9129 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9032..9051) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9086..9105 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 9297..9930 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 9977..10102 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10599..10832 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 11017..11061 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg 61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac 121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag 181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa 241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc 301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag 361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg 421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc 481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta 541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg 601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat 661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg 721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg 781 gatccggagc taccatggtg tctaaaggcg aggaggataa catggctata atcaaggaat 841 ttatgaggtt caaggtgcat atggagggat ctgtgaacgg tcacgagttc gaaatcgagg 901 gcgaaggcga ggggcgaccc tatgaaggga cacagacggc caaactgaag gtgaccaagg 961 gtggccccct gcctttcgcc tgggacatcc tgtcacccca gtttatgtac ggctctaagg 1021 cttacgttaa gcaccctgcc gatattccgg actacttgaa actgagcttc cctgaagggt 1081 tcaagtggga acgggtaatg aattttgagg atggcggtgt agtcacagtt acccaggaca 1141 gctctcttca ggacggagaa tttatctata aggttaaact tcggggcact aacttcccat 1201 cagacggccc cgtgatgcag aagaaaacta tgggctggga agccagctca gaacgcatgt 1261 atcccgagga cggagcactg aagggagaaa tcaagcagcg actcaagctg aaggatgggg 1321 gacattatga tgctgaggtc aaaaccacct ataaggccaa gaaacccgtt cagcttcctg 1381 gggcatacaa cgtgaatatc aaactggata ttacatccca taacgaagac tataccatcg 1441 tggaacagta cgagcgggcc gagggcagac atagcacagg gggcatggat gaattgtaca 1501 aggcggccac gcgtatgaag atggacaaaa agactatagt ttggtttaga agagacctaa 1561 ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt tttcctgtct 1621 tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca agatggtgga 1681 tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct gacctcactt 1741 taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt accggtgcta 1801 caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac cataccgtaa 1861 aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat ctattgtatg 1921 aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat tcttactgga 1981 agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg cggttgatgc 2041 caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta gggctggaga 2101 atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca ggatggagca 2161 atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat gcaaagaaca 2221 gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat ttcggggaaa 2281 taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg gcaagagata 2341 agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt ttaagagagt 2401 attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg ttgagtcatc 2461 ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga caaggcagga 2521 ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga tggatgcata 2581 acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat 2641 ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt gacatccttg 2701 gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc ttggacaatc 2761 ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa tggcttcccg 2821 agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct ttaaccgtac 2881 tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta gacatcgaca 2941 cagctcgtga gctactagct aaagctattt caagaacccg tggagcacag atcatgatcg 3001 gagcagcagc ccgggatcca ccggtcgcca ccggttcagg gagcggatcc ggctcaggga 3061 gtgcggccgc aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc 3121 gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa 3181 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 3241 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 3301 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 3361 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 3421 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 3481 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 3541 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt 3601 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 3661 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 3721 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc 3781 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 3841 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt 3901 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 3961 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 4021 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 4081 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg 4141 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag 4201 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc 4261 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg 4321 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc 4381 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc 4441 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt 4501 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc 4561 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag 4621 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata 4681 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 4741 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 4801 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 4861 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 4921 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 4981 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 5041 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 5101 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 5161 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 5221 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 5281 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 5341 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 5401 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 5461 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 5521 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 5581 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 5641 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 5701 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 5761 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 5821 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 5881 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 5941 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 6001 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 6061 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 6121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 6181 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 6241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 6301 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 6361 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 6421 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 6481 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 6541 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 6601 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 6661 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 6721 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 6781 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 6841 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag 6901 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 6961 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 7021 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 7081 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 7141 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 7201 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag 7261 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga 7321 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa 7381 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt 7441 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga 7501 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg 7561 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac 7621 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt 7681 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg 7741 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt 7801 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg 7861 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac 7921 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc 7981 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt 8041 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat 8101 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg 8161 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga 8221 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga 8281 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga 8341 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc 8401 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga 8461 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg 8521 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca 8581 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc 8641 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta 8701 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt 8761 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt 8821 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct 8881 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac 8941 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 9001 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 9061 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 9121 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac 9181 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa 9241 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga 9301 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca 9361 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac 9421 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa 9481 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag 9541 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct 9601 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg 9661 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg 9721 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct 9781 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 9841 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 9901 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 9961 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag 10021 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga 10081 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa 10141 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag 10201 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag 10261 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt 10321 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa 10381 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc 10441 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa 10501 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag 10561 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc 10621 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga 10681 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa 10741 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct 10801 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc 10861 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt 10921 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata 10981 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa 11041 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata 11101 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt 11161 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca 11221 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga 11281 gacagatcca ttcgattagt gaa //