LOCUS Exported 9132 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence). ACCESSION . VERSION . KEYWORDS pCW57.1-DUX4-WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9132) AUTHORS Jagannathan S, Shadle SC, Resnick R, Snider L, Tawil RN, van der Maarel SM, Bradley RK, Tapscott SJ TITLE Model systems of DUX4 expression recapitulate the transcriptional profile of FSHD cells. JOURNAL Hum Mol Genet. 2016 Oct 15;25(20):4419-4431. doi: 10.1093/hmg/ddw271. PUBMED 28171552 REFERENCE 2 (bases 1 to 9132) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9132 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 142..159 /label=L4440 /note="L4440 vector, forward primer" protein_bind 276..297 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 312..342 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 350..366 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 355..377 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 374..390 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 374..390 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 409..429 /label=T3 /note="T3 promoter, forward primer" promoter 411..429 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 457..683 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 684..864 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 911..1036 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1529..1762 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1947..1991 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2204..2321 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2375..2689 /label=tight TRE promoter /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" protein_bind 2383..2401 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2419..2437 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2454..2472 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2490..2508 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2526..2544 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2561..2579 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 2597..2615 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 2662..2686 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 2742..2751 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(4049..4065) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4050..4066) /label=pBluescriptKS /note="For pBluescript vector" protein_bind complement(4068..4192) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 4210..4227 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" promoter 4237..4747 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 4666..4684 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 4757..5353 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4757..4776) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5253..5273 /label=Puro-F /note="Puromycin resistance gene, forward primer" CDS 5354..5407 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 5408..6154 /codon_start=1 /product="improved tetracycline-controlled transactivator" /label=rtTA-Advanced /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline." /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" primer_bind complement(6194..6214) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6612..6623) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6828..7061 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7133..7254 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7170..7189) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7224..7243 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7294..7429 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7356..7375 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7429..7500 /gene="bla" /label=AmpR promoter CDS 7501..8361 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7719..7738) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8532..9120 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9021..9040 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 61 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 121 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 181 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 241 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 301 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 361 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct 421 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt 481 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc 541 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga 601 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta 661 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc 721 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 781 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 841 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa 901 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga 961 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga 1021 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg 1081 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga 1141 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat 1201 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa 1261 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc 1321 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc 1381 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat 1441 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg 1501 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag 1561 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt 1621 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt 1681 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat 1741 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca 1801 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca 1861 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa 1921 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat 1981 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca 2041 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga 2101 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg 2161 gacaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg 2221 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 2281 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca 2341 gcagagatcc actttggccg cgaatcgata tgtcgagttt actccctatc agtgatagag 2401 aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt tactccctat 2461 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag 2521 tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag tgatagagaa 2581 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg 2641 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagaattgg 2701 ctagaactag tggatccccc gggctgcagg aattcgccct tgccaccatg gccctcccga 2761 caccctcgga cagcaccctc cccgcggaag cccggggacg aggacggcga cggagactcg 2821 tttggacccc gagccaaagc gaggccctgc gagcctgctt tgagcggaac ccgtacccgg 2881 gcatcgccac cagagaacgg ctggcccagg ccatcggcat tccggagccc agggtccaga 2941 tttggtttca gaatgagagg tcacgccagc tgaggcagca ccggcgggaa tctcggccct 3001 ggcccgggag acgcggcccg ccagaaggcc ggcgaaagcg gaccgccgtc accggatccc 3061 agaccgccct gctcctccga gcctttgaga aggatcgctt tccaggcatc gccgcccggg 3121 aggagctggc cagagagacg ggcctcccgg agtccaggat tcagatctgg tttcagaatc 3181 gaagggccag gcacccggga cagggtggca gggcgcccgc gcaggcaggc ggcctgtgca 3241 gcgcggcccc cggcgggggt caccctgctc cctcgtgggt cgccttcgcc cacaccggcg 3301 cgtggggaac ggggcttccc gcaccccacg tgccctgcgc gcctggggct ctcccacagg 3361 gggctttcgt gagccaggca gcgagggccg cccccgcgct gcagcccagc caggccgcgc 3421 cggcagaggg gatctcccaa cctgccccgg cgcgcgggga tttcgcctac gccgccccgg 3481 ctcctccgga cggggcgctc tcccaccctc aggctcctcg gtggcctccg cacccgggca 3541 aaagccggga ggaccgggac ccgcagcgcg acggcctgcc gggcccctgc gcggtggcac 3601 agcctgggcc cgctcaagcg gggccgcagg gccaaggggt gcttgcgcca cccacgtccc 3661 aggggagtcc gtggtggggc tggggccggg gtccccaggt cgccggggcg gcgtgggaac 3721 cccaagccgg ggcagctcca cctccccagc ccgcgccccc ggacgcctcc gcctccgcgc 3781 ggcaggggca gatgcaaggc atcccggcgc cctcccaggc gctccaggag ccggcgccct 3841 ggtctgcact cccctgcggc ctgctgctgg atgagctcct ggcgagcccg gagtttctgc 3901 agcaggcgca acctctccta gaaacggagg ccccggggga gctggaggcc tcggaagagg 3961 ccgcctcgct ggaagcaccc ctcagcgagg aagaataccg ggctctgctg gaggagcttt 4021 agaagggcga attcgatatc aagcttatcg ataccgtcga cctcgaccat agtgactgga 4081 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 4141 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtttagtaat 4201 gaaccggtcc accaccacca ccaccactaa ggatccgggg ttggggttgc gccttttcca 4261 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag 4321 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt 4381 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg 4441 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac 4501 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc 4561 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga 4621 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg 4681 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc 4741 tccccagcaa ttcaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 4801 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 4861 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 4921 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 4981 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 5041 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 5101 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 5161 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 5221 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 5281 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 5341 caagcccggt gccgaaggta gaggttctct cctcacttgt ggtgatgttg aagaaaaccc 5401 tggtccaatg tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa 5461 tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca 5521 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga 5581 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt 5641 tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa 5701 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc 5761 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg 5821 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag 5881 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga 5941 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga 6001 gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga 6061 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct 6121 tgacgatttt gaccttgaca tgctccccgg gtaactaagt aaggatcgat ccaagatatc 6181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 6241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 6301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 6361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 6421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 6481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga 6541 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 6601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 6661 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 6721 cctccccgcc tgtttcgcct cggcgtccgg actagaggta cctttaagac caatgactta 6781 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 6841 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca 6901 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6961 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 7021 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 7081 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 7141 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 7201 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 7261 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 7321 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 7381 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct ttcaaatatg 7441 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 7501 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 7561 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 7621 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 7681 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 7741 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 7801 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 7861 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 7921 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 7981 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 8041 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 8101 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 8161 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 8221 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 8281 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 8341 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 8401 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 8461 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 8521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 8581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 8641 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 8701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 8761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 8821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 8881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 8941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 9001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 9061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 9121 aacgccagca ac //