LOCUS       Exported                9132 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  All-in-one lentivector with tet-inducible DUX4 (codon sequences 
            altered).
ACCESSION   .
VERSION     .
KEYWORDS    pCW57.1-DUX4-CA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Jagannathan S, Shadle SC, Resnick R, Snider L, Tawil RN, van der 
            Maarel SM, Bradley RK, Tapscott SJ
  TITLE     Model systems of DUX4 expression recapitulate the transcriptional 
            profile of FSHD cells.
  JOURNAL   Hum Mol Genet. 2016 Oct 15;25(20):4419-4431. doi: 
            10.1093/hmg/ddw271.
  PUBMED    28171552
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     141..158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    275..296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        311..341
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    349..365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     354..376
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     373..389
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     373..389
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     408..428
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        410..428
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        456..682
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             683..863
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    910..1035
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1528..1761
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1946..1990
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2374..2688
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2382..2400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2418..2436
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2453..2471
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2489..2507
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2525..2543
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2560..2578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2596..2614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2661..2685
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2741..2750
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(4048..4064)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4049..4065)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(4067..4191)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4209..4226
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4236..4746
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4665..4683
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4756..5352
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4756..4775)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5252..5272
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5353..5406
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5407..6153
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6193..6213)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6611..6622)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6827..7060
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7132..7253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7169..7188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7223..7242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7293..7428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7355..7374
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7428..7499
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7500..8360
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7718..7737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8531..9119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9020..9039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
       61 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      121 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      181 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      241 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      301 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      361 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
      421 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
      481 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
      541 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
      601 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
      661 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
      721 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      781 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      841 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      901 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      961 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1021 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1081 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1141 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1201 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1261 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1321 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1381 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1441 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1501 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1561 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1621 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1681 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1741 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1801 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1861 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1921 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1981 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2041 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2101 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2161 acaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca ctttggccgc gaatcgatat gtcgagttta ctccctatca gtgatagaga
     2401 acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc
     2461 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     2521 ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac
     2581 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
     2641 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattggc
     2701 tagaactagt ggatcccccg ggctgcagga attcgccctt gccaccatgg cattgcctac
     2761 accttcagac tctacgctgc ctgcagaggc taggggaaga ggtagacggc ggcgattggt
     2821 gtggactcca tcacaatccg aagctcttcg cgcatgcttc gagcgcaatc cctatccggg
     2881 gattgccaca agggagaggc ttgcacaggc tatcggaatc ccggaaccga gagtgcagat
     2941 ctggttccaa aatgaacgct ctcggcagct cagacagcat cgcagggagt cccgcccgtg
     3001 gccaggaaga aggggaccac ctgaaggaag aagaaaacgc acagcggtga ctggcagcca
     3061 aacggctctg ctgctccgcg ctttcgagaa agatcggttc cccggaattg ccgcacgcga
     3121 agaactcgcc agagaaactg ggctcccaga atcacgaata cagatttggt tccagaaccg
     3181 cagagcaaga cacccaggcc aggggggacg ggcacctgct caggccggtg gactctgctc
     3241 tgctgcccct gggggcggcc atccagcacc ttcctgggtg gctttcgctc atactggcgc
     3301 ttggggtacc gggctgcctg ctccgcatgt tccctgtgct ccaggggccc tcccgcaggg
     3361 agcgtttgtt tcccaggcag ctagggctgc acctgccctg caaccatcac aggcagcgcc
     3421 agctgaaggc atcagccaac ccgccccagc ccgcggagat tttgcttatg cagcgccagc
     3481 acctccagac ggtgccctga gccaccccca agcccccaga tggccccctc accctggtaa
     3541 gtcccgggaa gaccgcgatc cccaacgaga tggactgccc ggtccttgcg ctgtggccca
     3601 gccaggacct gctcaagccg gccctcaggg gcaaggagtg ctggccccac ctacaagcca
     3661 gggatctccc tggtggggtt ggggacgcgg acctcaggtt gctggagccg cttgggagcc
     3721 tcaggccgga gctgcaccgc cgccacaacc ggcccctccc gacgcgtcag cgtccgcccg
     3781 acaaggccag atgcagggaa tcccagcacc tagccaagct cttcaagagc ctgccccttg
     3841 gagcgcactg ccgtgtgggc tgctcctgga tgaactcctg gctagcccag aatttctcca
     3901 gcaggcacag ccactcctgg aaacagaagc tccgggagag ctcgaagcct ccgaagaagc
     3961 agcaagcctg gaggcacctc tttccgagga ggagtataga gcccttctgg aagaactttg
     4021 aaagggcgaa ttcgatatca agcttatcga taccgtcgac ctcgaccata gtgactggat
     4081 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
     4141 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg tttagtaatg
     4201 aaccggtcca ccaccaccac caccactaag gatccggggt tggggttgcg ccttttccaa
     4261 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     4321 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     4381 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     4441 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     4501 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     4561 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     4621 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     4681 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     4741 ccccagcaat tcaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4801 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4861 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     4921 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     4981 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     5041 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     5101 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     5161 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     5221 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     5281 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     5341 aagcccggtg ccgaaggtag aggttctctc ctcacttgtg gtgatgttga agaaaaccct
     5401 ggtccaatgt ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat
     5461 ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
     5521 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag
     5581 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
     5641 ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa
     5701 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
     5761 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
     5821 cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga
     5881 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
     5941 cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
     6001 aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac
     6061 atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt
     6121 gacgattttg accttgacat gctccccggg taactaagta aggatcgatc caagatatcg
     6181 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6241 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6301 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6361 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6421 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6481 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     6541 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6601 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6661 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6721 ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac
     6781 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6841 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     6901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7081 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     7141 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7201 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7261 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7321 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7381 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt
     7441 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7501 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7561 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7621 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7681 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7741 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7801 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7861 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7921 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7981 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8041 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8101 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8161 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8221 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8281 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8341 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8401 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8461 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8521 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8581 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8641 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8701 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8761 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8821 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8881 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8941 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9001 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9061 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9121 acgccagcaa cg
//
