LOCUS Exported 2932 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION BB1 with insert: promoter PRPP1B. ACCESSION . VERSION . KEYWORDS BB1_12_pRPP1B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2932) AUTHORS Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, Sauer M, Mattanovich D, Gasser B, Marx H TITLE GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris. JOURNAL BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3. PUBMED 29221460 REFERENCE 2 (bases 1 to 2932) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2932 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 40..57 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 41..57 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1268..1284) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1268..1284) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" terminator 1332..1363 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 1364..1466 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1467..2282 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1540..1559) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 2306..2333 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(2345..2932) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2425..2444) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gaagatcctt tgattttcta ccgaagaaag gcccacccgt gtaaaacgac ggccagttta 61 tctagtcagc ttgattctag ctgatcgtgg accggaaggt gagccagtga gttgattgca 121 gtccagttac gctggagtct gaggctcgtc ctgaatgata tgcggcctcg aattcgaaga 181 cgcggagcca cttttgcatt tgacacaaaa tacaaattta aggagggaga tcatcgttac 241 gaagaattta gggagggcgt gacgttccta tgcaactggt ctaaccttat tccgaaagat 301 ctattcaatt tattttgtta tctggttaag aatgactctg agttgggtgt tacgaatgtt 361 gaccaaattt cattgcgaat gaaattagag ggattgacac agctacgcaa tgctttggag 421 gctaagagac aagtgttaca gaaaacatct ttacctcaat cagctcccct tcctgactct 481 acggaccaga aggtgattgc aagatcaatc aggaacttca acaatgctcg tatctacaga 541 catactcaag aaaaactcta tgttgctatg attgctgaac tcaaacacat agagaagggc 601 ttactgaaag agtatcgctc taaagttgtt tcactaaaga gtatttggaa aaaagatgct 661 acatttacta attccttact actatcacta ggatttgatt cactggaaaa agtagaggat 721 tctaattttc aagacactgt catcctgttg tggatcatgc gaacactgaa tagagagtat 781 tatgataaag aagatcaggc agttttaccc gaatggttac ataacctcaa gaccaagttt 841 ccgttgtcta ctatcaacgt ggagacagag ccttacaaac ctttatacga acagttattt 901 cctccactag cagaaagaat tccagaggtc tatgccaaag gagactggag cctagagttc 961 atggcatatg ctggtgttat tctgagttac atcagctaca caagaggtgc caaacaggag 1021 tgcatactgg tagaaccaat ccagggtgca acatagtaag tgttgcattc aggatgctcg 1081 tcagtgcgcg aatttgacct cgacctctcg tgactgcgtt tcttcgttgg agaaaaaaaa 1141 tcgctagacc ataactagaa catttcatct ataaccatca acatggcgtc ttcgaattcg 1201 cgcgtgatct tacggcatta tacgtatgat cggtccacga tcagctagat tatctagtca 1261 gcttgatgtc atagctgttt cctgaggctc aatactgacc atttaaatca tacctgacct 1321 ccatagcaga aagtcaaaag cctccgaccg gaggcttttg acttgatcgg cacgtaagag 1381 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga 1441 gattttcagg agctaaggaa gctaaaatga gccatattca acgggaaacg tcttgcttga 1501 agccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 1561 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt 1621 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcaggc 1681 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 1741 atgatgcatg gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag 1801 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 1861 attcgattcc tgtttgtaat tgtcctttta acggcgatcg cgtatttcgt ctcgctcagg 1921 cgcaatcacg aatgaataac ggtttggttg gtgcgagtga ttttgatgac gagcgtaatg 1981 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact cttgccattc tcaccggatt 2041 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 2101 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 2161 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 2221 gtattgataa tcctgatatg aataaattgc agtttcactt gatgctcgat gagtttttct 2281 aatgaggacc taaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg 2341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga 2401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 2461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 2521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 2581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 2641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 2701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 2761 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 2821 ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 2881 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aa //