LOCUS       Exported                6546 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  An AAV vector that expresses nuclear localized eYFP under control of
            the c-fos promoter..
ACCESSION   .
VERSION     .
KEYWORDS    pOTTC589	- pAAV c-fos Nuc-eYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     CDS             complement(856..867)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1833..2549
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1878..1899)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2139..2158)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2486..2507
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2574..2594
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2598..2618
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2622..2642
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2673..3261
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2726..2746)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3144..3155)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3293..3769
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3492..3511)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3809..3949
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3809..3938
                     /label=AAV2 ITR
     rep_origin      4024..4479
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4111..4130)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4321..4342
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4496..4515)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4615..4637
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4675..4693)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4761..4865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4866..5726
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5084..5103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5897..6485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6386..6405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttcctttagg aacagaggct
      181 tcgagccttt aaggctgcgt acttgcttct cctaatacca gagactcaaa aaaaaaaaaa
      241 aagttccaga ttgctggaca atgacccggg tctcatccct tgaccctggg aaccgggtcc
      301 acattgaatc aggtgcgaat gttcgctcgc cttctctgcc tttcccgcct cccctccccc
      361 ggccgcggcc ccggttcccc ccctgcgctg caccctcaga gttggctgca gccggcgagc
      421 tgttcccgtc aatccctccc tcctttacac aggatgtcca tattaggaca tctgcgtcag
      481 caggtttcca cggccggtcc ctgttgttct ggggggggga ccatctccga aatcctacac
      541 gcggaaggtc taggagaccc cctaagatcc caaatgtgaa cactcatagg tgaaagatgt
      601 atgccaagac gggggttgaa agcctggggc gtagagttga cgacagagcg cccgcagagg
      661 gccttggggc gcgcttcccc ccccttccag ttccgcccag tgacgtagga agtccatcca
      721 ttcacagcgc ttctataaag gcgccagctg aggcgcctac tactccaacc gcgactgcag
      781 cgagcaactg agaagactgg atagagccgg cggttccgcg aacgagcagt gaccgcgctc
      841 ccacccagct ctgctctgca gctcccacca gtgtctaccc ctggacccct tgccgggctt
      901 tccccaaact tcgaccatga tgttctcggg tttcaacgcc gactacgagg cgtcatcctc
      961 ccgctgcagt agcgcctccc cggccgggga cagcctttcc tactaccatt ccccagccga
     1021 ctccttctcc agcatgggct ctcctgtcaa cacacaggtg agtttggctt tgtgtagccg
     1081 ccaggtccgc gctgagggtc gccgtggagg agacactggg gtgtgactcg caggggcggg
     1141 ggggtcttcc tttttcgctc tggagggaga ctggcgcggt cagagcagcc ttagcctggg
     1201 aacccaggac ttgtctgagc gcgtgcacac ttgtcatagt aagacttagt gaccccttcc
     1261 cgcgcggcag gtttattctg agtggcctgc ctgcattctt ctctcggccg acttgtttct
     1321 gagatcagcc ggggccaaca agtctcgagc aaagagtcgc taactagagt ttgggaggcg
     1381 gcaaaccgcg gcaatccccc ctcccggggc agcctggagc agggaggagg gaggagggag
     1441 gagggtgctg cgggcgggtg tgtaaggcag tttcattgat aaaaagcgag ttcattctgg
     1501 agactccgga gcagcgcctg cgtcagcgca gacgtcaggg atatttataa caaaccccct
     1561 ttcgagcgag tgatgccgaa gggataacgg gaacgcagca gtaggatgga ggggaaaggc
     1621 tgcgctgcgg aattcaaggg aggatattgg gagagctttt atctccgatg aggtgcatac
     1681 aggaagacat aagcagtctc tgaccggaat gcttctctct ccctgcttca tgcgacacta
     1741 gggccacttg ctccacctgt gtctggaacc tcctcgctca cctccgcttt cctctttttg
     1801 ttttgtttca ggacttttgc gcagatctct ccatggtgag caagggcgag gagctgttca
     1861 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1921 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1981 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
     2041 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2101 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2161 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2221 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     2281 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2341 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2401 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     2461 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2521 tcactctcgg catggacgag ctgtacaagt ccggcctgcg cagccgcgcc gacccaaaaa
     2581 agaagcgcaa ggtagacccc aagaagaagc gcaaggtaga tccaaaaaag aagagaaagg
     2641 tatgatagga attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     2701 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2761 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2821 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2881 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2941 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     3001 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     3061 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     3121 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     3181 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     3241 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca
     3301 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     3361 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     3421 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     3481 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     3541 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
     3601 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     3661 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     3721 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta
     3781 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     3841 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     3901 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     3961 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4021 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4081 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4141 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4201 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     4261 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4321 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     4381 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4441 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     4501 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4561 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4621 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4681 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4861 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4921 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4981 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5041 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5101 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5161 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5221 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5281 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5341 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5401 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5461 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5521 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5581 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5641 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5701 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5761 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6241 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6481 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6541 acatgt
//
