LOCUS       Exported                 629 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    DCT.VV.Orf51
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             398..439
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             449..466
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(489..506)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    495..606
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gtacccttca ccgaattcca gctgaccacc atgtacgaac ataggcctcc tagtttggag
       61 actaattctt tttcatcaac tagacgttta ttctcaaata gcgattggtg ttgtaaggat
      121 cctggtcgta gtaagttaac caacatggtg aattcttgca cactattgac gataggtgta
      181 gccgataaac aaatcatctt atggtttttt aatgcgatgg tcttagataa aaaattatat
      241 actgaacgag taggacggat cttaccatct tctttgatta atgatttaga aatgaagtta
      301 tgagcaattc ccggggatcc gaataaaggg tcaagacaat tctgcagata tccagcacag
      361 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
      421 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
      481 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
      541 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
      601 tgcatcgcat tgtctgagta ggtgtcatt
//
