LOCUS Exported 3608 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.. ACCESSION . VERSION . KEYWORDS pRSM12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3608) AUTHORS Molina RS, Tran TM, Campbell RE, Lambert GG, Salih A, Shaner NC, Hughes TE, Drobizhev M TITLE Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation. JOURNAL J Phys Chem Lett. 2017 Jun 15;8(12):2548-2554. doi: 10.1021/acs.jpclett.7b00960. Epub 2017 May 25. PUBMED 28530831 REFERENCE 2 (bases 1 to 3608) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3608 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(63..81) /label=T7 Term /note="T7 terminator, reverse primer" terminator 77..124 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 221..676 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(308..327) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 518..539 /label=F1ori-F /note="F1 origin, forward primer" promoter 702..806 /gene="bla" /label=AmpR promoter CDS 807..1667 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1025..1044) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1838..2426 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2327..2346 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2580..2597 /label=L4440 /note="L4440 vector, forward primer" primer_bind 2682..2701 /label=T7 /note="T7 promoter, forward primer" promoter 2682..2700 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2826..2843 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 2847..2879 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 2883..2906 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" ORIGIN 1 gaattcgaag cttgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc 61 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt 121 tttgctgaaa ggaggaacta tatccggatc tggcgtaata gcgaagaggc ccgcaccgat 181 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca 241 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 301 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 361 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 421 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 481 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 541 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 601 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 661 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 721 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 781 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 841 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 901 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 961 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 1021 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 1081 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 1141 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 1201 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 1261 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 1321 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 1381 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 1441 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 1501 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 1561 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 1621 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 1681 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 1741 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 1801 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 1861 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 1921 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 1981 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 2041 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 2101 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 2161 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 2221 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 2281 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 2341 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 2401 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 2461 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 2521 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 2581 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 2641 gttggccgat tcattaatgc aggatctcga tcccgcgaaa ttaatacgac tcactatagg 2701 gagaccacaa cggtttccct ctagaatcac cgagtttatt cttgacacct gatgcgatga 2761 atgatataat aggaaagtac tgttttgttt aactttaaga aggggatata catatgcggg 2821 gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc 2881 gggatctgta cgacgatgac gataaggatc cggcacttag caagcaatcc ctgcccagcg 2941 atatgaagct gatctatcac atggatggga acgttaatgg gcactctttc gtgatcaaag 3001 gggagggaga gggcaagcca tacgaaggca cacacactat taagctgcag gtcgtggaag 3061 gtagtcctct gcctttctcc gccgatatcc tgagcaccgt cttccagtac ggcaaccggt 3121 gctttacgaa gtacccaccc aacatcgtgg actactttaa gaatagctgc agcgggggcg 3181 gatacaagtt tggccggagc tttctgtatg aggacggtgc cgtgtgcacc gcgtcaggag 3241 atataacact gtctgccgac aaaaagtcat tcgaacacaa atctaaattc ctgggtgtga 3301 actttcccgc agacggcccc gtgatgaaga aagaaactac caattgggag ccatcctgtg 3361 agaaaatgac cccgaacggc atgaccttga ttggagacgt aaccggattc ctcctcaaag 3421 aggacggcaa gcggtataag tgtcagtttc acacattcca tgatgccaag gacaagagca 3481 aaaagatgcc tatgcccgac ttccactttg tgcagcataa gatcgagaga aaagatctgc 3541 ccgggagtat gcagacctgg cggctgaccg agcatgctgc tgcctgtaaa acatgcttca 3601 ctgaatag //