LOCUS Exported 3238 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION BB1 with insert: promoter PGUT1. ACCESSION . VERSION . KEYWORDS BB1_12_pGUT1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3238) AUTHORS Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, Sauer M, Mattanovich D, Gasser B, Marx H TITLE GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris. JOURNAL BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3. PUBMED 29221460 REFERENCE 2 (bases 1 to 3238) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3238 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..17 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1534..1550) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1534..1550) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" terminator 1598..1629 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 1630..1732 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1733..2548 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1806..1825) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 2572..2599 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(2611..3198) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2691..2710) /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3238..17 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" ORIGIN 1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt 61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata 121 tgcggcctcg aattcgaaga cgcggagata aaaaaatatg gcaactttca ccagcttagc 181 tctccagact ttttcttccc gaatttttgg ttccgagaaa tggacctttg ctagccggtg 241 ggaaataact tgagatgcat ggacggaatc aaacacggaa aaatctaggt catcctacag 301 caaacacctg caaggccggg aaagaattgc tcggtatttt ctattttggg agatttgttg 361 gcacggaaga atcggtaact ttactaatcc aatactccgc tcctgactgt ttcaagtcgg 421 accccaactt tcaagtgacc caatttagca gcctgcattc tcttgatttt atgggggaaa 481 ctaacaatag tgttgccttg attttaagtg gcattgttct ttgaaatcga aattggggat 541 aacgtcatac cgaaaggtaa acaacttcgg ggaattgccc tggttaaaca tttattaagc 601 gagataaata ggggatagcg agataggggg cggagaagaa gaagggtgtt aaattgctga 661 aatctctcaa tctggaagaa acggaataaa ttaactcctt cctgagataa taagatccga 721 ctctgctatg accccacacg gtactgacct cggcataccc cattggatct ggtgcgaagc 781 aacaggtcct gaaaccttta tcacgtgtag tagattgacc ttccagcaaa aaaaggcatt 841 atatattttg ttgttgaagg ggtgagggga ggtgcaggtg gttcttttat tcgtcttgta 901 gttaattttg ccggggttgc ggagcgtcaa aagtttgccc gatctgatag cttgcaagat 961 gccaccgctt atccaacgca cttcagagag cttgccgtag aaagaacgtt ttcctcgtag 1021 tattccagca cttcatggtg aagtcgctat ttcaccgaag ggggggtatt aaggttgcgc 1081 accccctccc cacaccccag aatcgtttat tggctgggtt caatggcgtt tgagttagca 1141 cattttttcc ttaaacaccc tccaaacacg gataaaaatg catgtgcatc ctgaaactgg 1201 tagagatgcg tactccgtgc tccgataata acagtggtgt tggggttgct gttagctcac 1261 gcactccgtt tttttttcaa ccagcaaaat tcgatgggga gaaacttggg gtactttgcc 1321 gactcctcca ccatactggt atataaataa tactcgccca cttttcgttt gctgctttta 1381 tatttcaagg actgaaaaag actcttcttc tactttttca cactatacca cagatatatc 1441 tactatacat ggcgtcttcg aattcgcgcg tgatcttacg gcattatacg tatgatcggt 1501 ccacgatcag ctagattatc tagtcagctt gatgtcatag ctgtttcctg aggctcaata 1561 ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg 1621 cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact 1681 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca 1741 tattcaacgg gaaacgtctt gcttgaagcc gcgattaaat tccaacatgg atgctgattt 1801 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt 1861 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa 1921 tgatgttaca gatgagatgg tcaggctaaa ctggctgacg gaatttatgc ctcttccgac 1981 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg 2041 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc 2101 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacgg 2161 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttggtgc 2221 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca 2281 taaactcttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa 2341 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc 2401 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt 2461 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt 2521 tcacttgatg ctcgatgagt ttttctaatg aggacctaaa tgtaatcacc tggctcacct 2581 tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 2641 tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 2701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 2761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 2821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 2881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 2941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 3001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 3061 tggtatctgc gctctgctga agccagttac ctcggaaaaa gagttggtag ctcttgatcc 3121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 3181 agaaaaaaag gatctcaaga agatcctttg attttctacc gaagaaaggc ccacccgt //