LOCUS Exported 2938 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION BB1 with insert: promoter PFDH1. ACCESSION . VERSION . KEYWORDS BB1_12_pFDH1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2938) AUTHORS Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, Sauer M, Mattanovich D, Gasser B, Marx H TITLE GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris. JOURNAL BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3. PUBMED 29221460 REFERENCE 2 (bases 1 to 2938) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2938 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..17 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1234..1250) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1234..1250) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" terminator 1298..1329 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 1330..1432 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1433..2248 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1506..1525) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 2272..2299 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(2311..2898) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2391..2410) /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2938..17 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" ORIGIN 1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt 61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata 121 tgcggcctcg aattcgaaga cgcggagaaa tggcagaagg atcagcctgg acgaagcaac 181 cagttccaac tgctaagtaa agaagatgct agacgaagga gacttcagag gtgaaaagtt 241 tgcaagaaga gagctgcggg aaataaattt tcaatttaag gacttgagtg cgtccatatt 301 cgtgtacgtg tccaactgtt ttccattacc taagaaaaac ataaagatta aaaagataaa 361 cccaatcggg aaactttagc gtgccgtttc ggattccgaa aaacttttgg agcgccagat 421 gactatggaa agaggagtgt accaaaatgg caagtcgggg gctactcacc ggatagccaa 481 tacattctct aggaaccagg gatgaatcca ggtttttgtt gtcacggtag gtcaagcatt 541 cacttcttag gaatatctcg ttgaaagcta cttgaaatcc cattgggtgc ggaaccagct 601 tctaattaaa tagttcgatg atgttctcta agtgggactc tacggctcaa acttctacac 661 agcatcatct tagtagtccc ttcccaaaac accattctag gtttcggaac gtaacgaaac 721 aatgttcctc tcttcacatt gggccgttac tctagccttc cgaagaacca ataaaaggga 781 ccggctgaaa cgggtgtgga aactcctgtc cagtttatgg caaaggctac agaaatccca 841 atcttgtcgg gatgttgctc ctcccaaacg ccatattgta ctgcagttgg tgcgcatttt 901 agggaaaatt taccccagat gtcctgattt tcgagggcta cccccaactc cctgtgctta 961 tacttagtct aattctattc agtgtgctga cctacacgta atgatgtcgt aacccagtta 1021 aatggccgaa aaactattta agtaagttta tttctcctcc agatgagact ctccttcttt 1081 tctccgctag ttatcaaact ataaacctat tttacctcaa atacctccaa catcacccac 1141 ttaaacacat ggcgtcttcg aattcgcgcg tgatcttacg gcattatacg tatgatcggt 1201 ccacgatcag ctagattatc tagtcagctt gatgtcatag ctgtttcctg aggctcaata 1261 ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg 1321 cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact 1381 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca 1441 tattcaacgg gaaacgtctt gcttgaagcc gcgattaaat tccaacatgg atgctgattt 1501 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt 1561 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa 1621 tgatgttaca gatgagatgg tcaggctaaa ctggctgacg gaatttatgc ctcttccgac 1681 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg 1741 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc 1801 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacgg 1861 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttggtgc 1921 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca 1981 taaactcttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa 2041 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc 2101 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt 2161 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt 2221 tcacttgatg ctcgatgagt ttttctaatg aggacctaaa tgtaatcacc tggctcacct 2281 tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 2341 tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 2401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 2461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 2521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 2581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 2641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 2701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 2761 tggtatctgc gctctgctga agccagttac ctcggaaaaa gagttggtag ctcttgatcc 2821 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2881 agaaaaaaag gatctcaaga agatcctttg attttctacc gaagaaaggc ccacccgt //