LOCUS       Exported                6436 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  S. cerevisiae expression of F-box protein TIR1-9myc under control of
            the ADH1 promoter, TRP1 marker (for use with the AID degron system).
ACCESSION   .
VERSION     .
KEYWORDS    YIp204-PADH1-atTIR1-9myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6436)
  TITLE     Unpublished Ulrich lab AID plasmids
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..397
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             2225..2254
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2261..2290
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2297..2326
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2345..2374
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2381..2410
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2417..2446
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2465..2494
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2501..2530
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2537..2566
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2664..2680)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2664..2680)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2677..2699)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2688..2704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2712..2742)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2757..2778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2895..2912)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3066..3654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3146..3165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3825..4685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4448..4467
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4686..4790)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4858..4876
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4878..4979
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             4980..5654
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(5773..5795)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5895..5914
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6108..6130
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6122..6139
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6123..6139
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca
       61 atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac
      121 agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc
      181 cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact
      241 tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt
      301 attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg
      361 cacaatattt caagctatac caagcataca atcaactatc tcatatacaa ctagtatgca
      421 gaagcgaata gccttgtcgt ttccagaaga ggtactagag catgtgttct cgtttattca
      481 gctggataag gataggaact cagtctctct ggtgtgcaag tcatggtacg agatcgagcg
      541 gtggtgcagg aggaaagtct tcatcgggaa ctgctacgcc gtgagtccag cgacggtgat
      601 taggaggttc ccgaaagtga gatccgtgga gcttaaagga aaacctcact ttgctgactt
      661 taatttggta cctgacggat ggggaggtta cgtgtatcca tggattgagg ccatgtcttc
      721 gtcttacacg tggcttgaag agataaggct gaagaggatg gtggtcaccg acgattgctt
      781 ggagctcata gccaagtctt ttaagaattt taaggttctt gtgctttctt cctgcgaagg
      841 cttctccacc gatggtctcg ctgctatcgc tgccacttgc aggaatctga aagagcttga
      901 cttacgagag agtgatgttg acgacgttag tggccactgg cttagccatt tcccagatac
      961 atacacttct ttggtatcac tcaatatatc ttgcttagca tctgaggtca gtttctctgc
     1021 tctggaaagg ctggtgacta ggtgtcccaa tctcaagtct ctcaagctta accgagctgt
     1081 tccacttgaa aaattggcta ctttacttca aagagcacct caattggagg aattgggcac
     1141 tggtgggtac actgcagaag tgcgaccaga tgtttactct ggtttatctg tagcgctctc
     1201 tgggtgcaag gaattgaggt gcttatctgg attttgggat gctgttcctg cctatcttcc
     1261 agcagtttat tcggtttgca gtcggcttac aactttgaat ctgagttatg caacagtcca
     1321 gagctatgat cttgtcaagc ttctttgtca atgccctaaa ctgcagcgcc tctgggtgct
     1381 tgactacatc gaggatgctg gtcttgaggt gcttgcttca acctgcaagg acctacgcga
     1441 gctgagagtg tttccgtccg agccttttgt catggaacca aatgtggcat tgacggaaca
     1501 ggggcttgtc tccgtctcca tgggctgtcc aaaactcgag tcggttctct acttctgccg
     1561 tcaaatgacc aatgctgcat tgataaccat tgctaggaac cgtcccaaca tgactcgctt
     1621 ccgtttgtgc atcattgagc caaaagcccc agactatctg actctagagc cactggatat
     1681 tggatttgga gccatagtag agcactgcaa ggatctccgt cgcctctctc tatctggcct
     1741 cttgaccgac aaggtttttg aatacattgg gacatatgcc aagaagatgg aaatgctctc
     1801 agtggcattt gcaggagaca gtgacttagg catgcatcat gttttgtccg ggtgcgatag
     1861 cttgaggaaa ctagagataa gggactgccc gtttggagac aaggcgcttt tggccaatgc
     1921 ttcaaagctg gagacaatgc gatccctttg gatgtcttct tgttccgtga gttttggagc
     1981 ctgcaagtta ctaggacaga agatgccaaa gctgaatgtg gaagtcatcg atgaacgggg
     2041 tgcaccggac tcgagaccag agagctgccc tgttgagaga gtcttcatat accgaacagt
     2101 ggctggtcct cgatttgaca tgcctggctt cgtctggaac atggaccaag actcaacaat
     2161 gaggttttcc aggcaaatca ttactattaa cggattagtc gactccggtt ctgctgctag
     2221 tggtgaacaa aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga
     2281 ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gatcctctag
     2341 aggtgaacaa aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga
     2401 ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gatcctctag
     2461 aggtgaacaa aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga
     2521 ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gatccactag
     2581 ctaaaaagtg gaacgatcat tcaagagatc cccggggatc ctctagagtc gacctgcagg
     2641 catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2701 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2761 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2821 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2881 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2941 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3001 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3061 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3121 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3241 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3301 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3361 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3421 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3481 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3541 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3601 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3661 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3721 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3781 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3841 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3901 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3961 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4021 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4081 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4141 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4201 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4261 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4321 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4381 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4441 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4501 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4561 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4621 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4681 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4741 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4801 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     4861 aggcgtatca cgaggccaat tcggtcgaaa aaagaaaagg agagggccaa gagggagggc
     4921 attggtgact attgagcacg tgagtatacg tgattaagca cacaaaggca gcttggagta
     4981 tgtctgttat taatttcaca ggtagttctg gtccattggt gaaagtttgc ggcttgcaga
     5041 gcacagaggc cgcagaatgt gcactagatt ccgatgctga cttgctgggt attatatgtg
     5101 tgcccaatag aaagagaaca attgacccgg ttattgcaag gaaaatttca agtcttgtaa
     5161 aagcatataa aaatagttca ggcactccga aatacttggt tggcgtgttt cgtaatcaac
     5221 ctaaggagga tgttttggct ctggtcaatg attacggcat tgatatcgtc caactgcatg
     5281 gagatgagtc gtggcaagaa taccaagagt tcctcggttt gccagttatt aaaagactcg
     5341 tatttccaaa agactgcaac atactactca gtgcagcttc acagaaacct cattcgttta
     5401 ttcccttgtt tgattcagaa gcaggtggga caggtgaact tttggattgg aactcgattt
     5461 ctgactgggt tggaaggcaa gagagccccg agagcttaca ttttatgtta gctggtggac
     5521 tgacgccaga aaatgttggt gatgcgctta gattaaatgg cgttattggt gttgatgtaa
     5581 gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa
     5641 atgctaagaa ataggttatt actgagtagt atttatttaa gtattgtttg tgcacttgcc
     5701 tgcaagcctt ttgaaaagca agcataaaag atcgcccttt cgtctcgcgc gtttcggtga
     5761 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     5821 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     5881 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     5941 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     6001 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6061 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6121 ttgtaaaacg acggccagtg aattcgggtg tacaatatgg acttcctctt ttctggcaac
     6181 caaacccata catcgggatt cctataatac cttcgttggt ctccctaaca tgtaggtggc
     6241 ggaggggaga tatacaatag aacagatacc agacaagaca taatgggcta aacaagacta
     6301 caccaattac actgcctcat tgatggtggt acataacgaa ctaatactgt agccctagac
     6361 ttgatagcca tcatcatatc gaagtttcac tacccttttt ccatttgcca tctattgaag
     6421 taataatagg cgcatg
//
