LOCUS Exported 2104 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION BB1 with insert: RPS3tt. ACCESSION . VERSION . KEYWORDS BB1_34_RPS3tt SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2104) AUTHORS Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, Sauer M, Mattanovich D, Gasser B, Marx H TITLE GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris. JOURNAL BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3. PUBMED 29221460 REFERENCE 2 (bases 1 to 2104) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2104 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..17 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(400..416) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(400..416) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" terminator 464..495 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 496..598 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 599..1414 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(672..691) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 1438..1465 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1477..2064) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1557..1576) /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2104..17 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" ORIGIN 1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt 61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata 121 tgcggcctcg aattcgaaga cgcgcttctg tttaattctg taggataaac gaaataactg 181 tatctttata atcatcctta agatagccct ggactcgtca gacattagtt cgcgcacatt 241 attttcctcc actgtctgca aacagccaca cggcatttcc ctctctagtt tcatttatca 301 aagtagcaca acacgctgcg tcttcgaatt cgcgcgtgat cttacggcat tatacgtatg 361 atcggtccac gatcagctag attatctagt cagcttgatg tcatagctgt ttcctgaggc 421 tcaatactga ccatttaaat catacctgac ctccatagca gaaagtcaaa agcctccgac 481 cggaggcttt tgacttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag 541 atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat 601 gagccatatt caacgggaaa cgtcttgctt gaagccgcga ttaaattcca acatggatgc 661 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta 721 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt 781 tgccaatgat gttacagatg agatggtcag gctaaactgg ctgacggaat ttatgcctct 841 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat 901 cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt 961 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt 1021 taacggcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt 1081 tggtgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga 1141 aatgcataaa ctcttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact 1201 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg 1261 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc 1321 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt 1381 gcagtttcac ttgatgctcg atgagttttt ctaatgagga cctaaatgta atcacctggc 1441 tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc cataggctcc gcccccctga 1501 cgagcatcac aaaaatcgat gctcaagtca gaggtggcga aacccgacag gactataaag 1561 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 1621 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 1681 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 1741 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 1801 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 1861 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 1921 agtatttggt atctgcgctc tgctgaagcc agttacctcg gaaaaagagt tggtagctct 1981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 2041 acgcgcagaa aaaaaggatc tcaagaagat cctttgattt tctaccgaag aaaggcccac 2101 ccgt //