LOCUS Exported 2215 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION empty BB2 for transcription unit cloning. ACCESSION . VERSION . KEYWORDS BB2_BC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2215) AUTHORS Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, Sauer M, Mattanovich D, Gasser B, Marx H TITLE GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris. JOURNAL BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3. PUBMED 29221460 REFERENCE 2 (bases 1 to 2215) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2215 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 49..153 /gene="bla" /label=AmpR promoter CDS 154..1014 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(372..391) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1240..1828 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1729..1748 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1982..1999 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 ccggtgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 61 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 121 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 181 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 241 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 301 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 361 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 421 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 481 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 541 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 601 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 661 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 721 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 781 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 841 gctgataaat ctggagccgg tgagcgtggc tctcgcggta tcattgcagc actggggcca 901 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 961 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 1021 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 1081 atctaggtga agatcctttt tgataatctc atgaacaata aaactgtctg cttacataaa 1141 cagtaataca aggggtgtag atctcatgac caaaatccct taacgtgagt tttcgttcca 1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1381 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 1981 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 2041 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 2101 tgagcgcaac actagtcgca aaaaaccccg cccctgacag ggcggggttt tttcgcggtc 2161 tccccgggga ggcgtcttcg gatccctcga ggaagacgcc gctaattgga gacca //