LOCUS       Exported                2922 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  BB1 with insert: promoter PPDC1.
ACCESSION   .
VERSION     .
KEYWORDS    BB1_12_pPDC1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      138..165
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(177..764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(257..276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     804..821
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     805..821
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2022..2038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2022..2038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      2086..2117
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        2118..2220
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2221..114
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2294..2313)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
       61 aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt ctaatgagga
      121 cctaaatgta atcacctggc tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc
      181 cataggctcc gcccccctga cgagcatcac aaaaatcgat gctcaagtca gaggtggcga
      241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttacctcg
      661 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      721 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgattt
      781 tctaccgaag aaaggcccac ccgtgtaaaa cgacggccag tttatctagt cagcttgatt
      841 ctagctgatc gtggaccgga aggtgagcca gtgagttgat tgcagtccag ttacgctgga
      901 gtctgaggct cgtcctgaat gatatgcggc ctcgaattcg aagacgcgga gagcaaataa
      961 acgcaaagag caacatatta ggataaacca acaggttaag tatagagcta aagcattgct
     1021 gagcaatatt tcggatcaac atcaacagaa tagatcgtca ccacgaagta tatccaacta
     1081 ttccccaaga atccaggctt atccctcaag aatggcctct ccatctcctt caatgaagat
     1141 ggcattgaca aactccgtca gcttgcagga tacaatggat gcggaactag aagcgttggc
     1201 aaacgaacaa tattacgtca tgctagatat ccttaaggga ttttgcgatc tttcatttga
     1261 catggtaaac atattttcta tccagatccc tgaggtatta catctcctct tcggctttgg
     1321 tgcagggtca ctggccttaa cgggagtcgt atccaaaacg agacaagaac taataaataa
     1381 acagatataa gggacaagca cacgattacc caatcacttg atatgcacca atttgttccg
     1441 ttgtttatgc catatttacc gaattttctt cccaggtttt tccgaatgga catctgtagt
     1501 ccactttttg gttatcataa tcgtcccaca agtcgtggat ttaaccagaa cctagtaatt
     1561 ttaagttcgc tattaatcac tcagaatggt cgcaccttgc tattggccaa gtctggagtc
     1621 gccagctacc acctcagagg ctacatagac ctcccaatgt catctcctca gtgcgctctt
     1681 caatctcgtg tcttttccgt taaaactccg ttcgtttcac cctatactgc ccctggttgt
     1741 gcagctctta ccacttcgcg ccgctactat ccgtagtggt cgagccgcat caatatcacg
     1801 ttgaaataga ataactccct acaaaagccg cacgcaacca tcaaatctat ataaggaacc
     1861 tcaaatatct agcaacatct tttcaattta ctacaacata ttcgttaatc atcaatcaat
     1921 tagctagtac acaaccatgg cgtcttcgaa ttcgcgcgtg atcttacggc attatacgta
     1981 tgatcggtcc acgatcagct agattatcta gtcagcttga tgtcatagct gtttcctgag
     2041 gctcaatact gaccatttaa atcatacctg acctccatag cagaaagtca aaagcctccg
     2101 accggaggct tttgacttga tcggcacgta agaggttcca actttcacca taatgaaata
     2161 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
     2221 atgagccata ttcaacggga aacgtcttgc ttgaagccgc gattaaattc caacatggat
     2281 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2341 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2401 gttgccaatg atgttacaga tgagatggtc aggctaaact ggctgacgga atttatgcct
     2461 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2521 atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2581 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2641 tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2701 gttggtgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2761 gaaatgcata aactcttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2821 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2881 ggaatcgcag accgatacca ggatcttgcc atcctatgga ac
//
