LOCUS Exported 11322 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.. ACCESSION . VERSION . KEYWORDS pCSN067 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11322) AUTHORS Verwaal R, Buiting-Wiessenhaan N, Dalhuijsen S, Roubos JA TITLE CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae. JOURNAL Yeast. 2017 Sep 8. doi: 10.1002/yea.3278. PUBMED 28886218 REFERENCE 2 (bases 1 to 11322) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11322 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 187..467 /gene="S. cerevisiae TRP1" /label=TRP1 promoter CDS 468..1142 /codon_start=1 /gene="S. cerevisiae TRP1" /product="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /label=TRP1 /note="yeast auxotrophic marker" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" rep_origin complement(1241..1696) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1378..1399) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1590..1609 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1826..1848 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1840..1857 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1841..1857 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 1867..1886 /label=T7 /note="T7 promoter, forward primer" promoter 1867..1885 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2900..6583 /codon_start=1 /product="CRISPR-associated protein from Lachnospiraceae bacterium ND2006 (Zetsche et al., 2015)" /label=LbCpf1 /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS VKH" CDS 6596..6616 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" protein_bind complement(6965..6998) /label=lox66 /bound_moiety="Cre recombinase" /note="Right element (RE) mutant of loxP (Araki et al., 2010). Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." gene 7044..8400 /label=kanMX /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" promoter 7044..7387 /label=TEF promoter /note="Ashbya gossypii TEF promoter" CDS 7388..8197 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(7455..7474) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 8203..8400 /label=TEF terminator /note="Ashbya gossypii TEF terminator" protein_bind complement(8448..8481) /label=lox71 /bound_moiety="Cre recombinase" /note="Left element (LE) mutant of loxP (Araki et al., 2010). Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind complement(8548..8568) /label=T3 /note="T3 promoter, forward primer" promoter complement(8548..8566) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(8587..8603) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8587..8603) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8600..8622) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8611..8627 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8635..8665) /label=lac promoter /note="promoter for the E. coli lac operon" primer_bind complement(8818..8835) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8989..9577) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9069..9088) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9748..10608) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10371..10390 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10609..10713) /gene="bla" /label=AmpR promoter misc_feature 10750..11253 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" primer_bind 11295..11313 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga 1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 1861 cgcgcgtaat acgactcact atagggcgaa ttgggtacct tttctttttt tgcggtcacc 1921 cccatgtggc ggggaggcag aggagtaggt agagcaacga atcctactat ttatccaaat 1981 tagtctagga actctttttc tagatttttt agatttgagg gcaagcgctg ttaacgactc 2041 agaaatgtaa gcactacgga gtagaacgag aaatccgcca taggtggaaa tcctagcaaa 2101 atcttgctta ccctagctag cctcaggtaa gctagcctta gcctgtcaaa tttttttcaa 2161 aatttggtaa gtttctacta gcaaagcaaa cacggttcaa caaaccgaaa actccactca 2221 ttatacgtgg aaaccgaaac aaaaaaacaa aaaccaaaat actcgccaat gagaaagttg 2281 ctgcgtttct actttcgagg aagaggaact gagaggattg actacgaaag gggcaaaaac 2341 gagtcgtatt ctcccattat tgtctgctac cacgcggtct agtagaataa gcaaccagtc 2401 aacgctaaga caggtaatca aaataccagt ctgctggcta cgggctagtt tttacctctt 2461 ttagaaccca ctgtaaaagt ccgttgtaaa gcccgttctc actgttggcg tttttttttt 2521 tttggtttag tttcttattt ttcatttttt tctttcatga ccaaaaacaa acaaatctcg 2581 cgatttgtac tgcggccact ggggcgtggc caaaaaaatg acaaatttag aaaccttagt 2641 ttctgatttt tcctgttatg aggagatatg ataaaaaata ttactgcttt attgtttttt 2701 ttttatctac tgaaatagag aaacttaccc aaggaggagg caaaaaaaag agtatatata 2761 cagcagctac cattcagatt ttaatatatt cttttctctt cttctacact attattataa 2821 taattttact atattcattt ttagcttaaa acctcataga atattattct tcagtcactc 2881 gcttaaatac ttatcaaaaa tgtctaagtt ggaaaaattc accaactgtt actctttgtc 2941 taagactttg agattcaagg ccatcccagt tggtaagacc caagaaaaca tcgacaacaa 3001 gagactatta gttgaagatg aaaagagagc tgaagactac aagggtgtca agaaattgtt 3061 ggacagatac tacttgtctt ttatcaacga cgttttgcat tccatcaagc taaagaactt 3121 gaataactac atctctttgt tcagaaagaa gactagaact gaaaaggaaa ataaggaatt 3181 ggaaaacttg gaaatcaact tgagaaagga aattgctaag gctttcaagg gtaatgaagg 3241 ttacaagtct ttattcaaga aagacatcat tgaaaccatt ttgccagaat ttttggatga 3301 taaggatgaa attgctttgg ttaactcttt caacggtttc accactgctt tcactggttt 3361 cttcgacaac agagaaaaca tgttctccga ggaagctaaa tccacttcta ttgctttcag 3421 atgtatcaac gaaaacttga cccgttacat ctctaacatg gacatttttg aaaaggtcga 3481 cgccatcttt gacaagcacg aagtccaaga aatcaaggaa aagatcttaa actccgacta 3541 cgatgtcgaa gatttcttcg aaggtgaatt cttcaacttt gttttaaccc aagaaggtat 3601 cgatgtctac aacgccatta tcggtggttt tgtcactgaa tctggtgaaa agatcaaggg 3661 tttgaacgaa tacattaact tgtacaacca aaagaccaaa caaaaattgc caaagttcaa 3721 gccattgtac aagcaagttt tgtctgacag agaatctttg tctttttacg gtgaagggta 3781 cacctctgac gaagaagtct tggaagtctt cagaaacact ttgaacaaga actctgaaat 3841 cttctcctcc atcaagaagt tagaaaagtt gttcaagaac ttcgatgaat actcttctgc 3901 tggtatcttc gttaagaacg gtccagccat ctctaccatt tctaaggata tctttggtga 3961 atggaacgtc attagagaca aatggaacgc tgaatacgat gacatccatt tgaagaaaaa 4021 ggctgttgtc accgaaaagt acgaagacga cagaagaaaa tccttcaaga agatcggttc 4081 cttctccttg gaacaattac aagaatacgc cgatgccgat ttgtccgttg tcgaaaaatt 4141 gaaggaaatt attattcaaa aggttgatga aatttacaaa gtttacggtt cctctgaaaa 4201 gttattcgat gctgatttcg tcttggaaaa gtctttgaag aagaacgacg ctgttgtcgc 4261 tatcatgaag gacttgttgg actctgtcaa atctttcgaa aactatatca aggccttctt 4321 cggtgaaggt aaggaaacta acagagatga atccttctac ggtgactttg tcttggctta 4381 cgatattttg ttgaaggttg accacatcta cgatgccatc agaaactacg ttactcaaaa 4441 gccatactct aaggacaaat tcaagttgta cttccaaaac ccacaattca tgggtggttg 4501 ggataaggac aaggaaactg actacagagc taccattttg agatacggtt ccaagtacta 4561 cttggccatc atggacaaga agtacgccaa gtgtttgcaa aagattgaca aggacgatgt 4621 caacggtaac tacgaaaaga ttaactacaa gttgttgcca ggtccaaaca agatgttgcc 4681 aaaggttttc ttctccaaaa agtggatggc ttactacaac ccatctgaag acatccaaaa 4741 gatctacaag aacggtactt tcaaaaaggg tgacatgttc aacttaaacg actgtcacaa 4801 gttgatcgac ttcttcaagg actccatctc tagataccca aaatggtcca acgcttacga 4861 tttcaacttc tctgaaactg aaaaatacaa ggatattgct ggtttctacc gtgaagtcga 4921 ggaacaaggt tataaggttt ctttcgaatc cgcttctaag aaagaagttg acaaattagt 4981 cgaagaaggt aagttgtaca tgttccaaat ctacaacaaa gatttctccg acaagtctca 5041 cggtactcca aacttgcaca ccatgtactt caagttgcta ttcgatgaaa acaaccacgg 5101 tcaaatcaga ttgtctggtg gtgctgaatt gttcatgaga cgtgcttctc taaagaagga 5161 agaattagtc gtccacccag ctaactctcc aattgccaac aagaacccag acaaccctaa 5221 gaagaccacc actttgtcct acgacgttta caaggacaag agattctccg aagaccaata 5281 cgaattgcac attccaattg ctatcaacaa gtgtccaaag aacatcttca agatcaacac 5341 tgaagtcaga gttttgttaa agcacgatga caacccttac gttattggta tcgaccgtgg 5401 tgaaagaaat ttgttgtaca ttgttgttgt tgacggtaag ggtaacatcg ttgaacaata 5461 ctccttgaac gaaatcatca acaacttcaa cggtattaga atcaagactg attaccactc 5521 tttgttggat aagaaggaaa aggaacgttt tgaagctcgt caaaactgga cctctattga 5581 aaacatcaaa gaattgaagg ctggttacat cagtcaagtt gtccacaaga tctgtgaatt 5641 ggtcgagaag tacgatgccg ttattgcctt ggaagatttg aactctggtt ttaagaactc 5701 tcgtgtcaag gttgaaaagc aagtctacca aaagttcgaa aagatgttaa tcgacaaatt 5761 gaactacatg gttgacaaga aatccaaccc atgtgctacc ggtggtgctt tgaaaggtta 5821 ccaaatcacc aacaaattcg aatctttcaa atctatgtcc actcaaaacg ggttcatctt 5881 ctacattcca gcttggttga cctccaagat cgacccatct accggtttcg ttaacttgtt 5941 gaagaccaag tacacttcca ttgctgattc caagaagttc atctcttctt tcgacagaat 6001 catgtacgtt ccagaagaag acttgttcga attcgccttg gactataaga acttctccag 6061 aaccgatgct gactacatta agaaatggaa attgtactcc tacggtaaca gaatcagaat 6121 tttcagaaac ccaaagaaaa acaacgtttt cgattgggaa gaagtttgtt tgacttctgc 6181 ctacaaggaa ttattcaaca aatacggtat caactaccaa caaggtgata tcagagcttt 6241 gttgtgtgaa caatctgaca aggctttcta ctcttccttc atggctttga tgtccttgat 6301 gttgcaaatg agaaactcca tcactggtag aactgatgtc gacttcctca tttctccagt 6361 taagaattct gacggtattt tctacgactc tagaaattac gaagctcaag aaaacgctat 6421 tttgccaaag aacgctgatg ctaacggtgc ttacaatatt gctagaaagg ttttgtgggc 6481 tatcggtcaa ttcaagaagg ctgaagacga aaagctagac aaggtcaaga ttgctatttc 6541 taacaaggaa tggttggaat acgctcaaac ctccgtcaag cactccagag ctgatccaaa 6601 gaagaagaga aaggtataaa ggagttaaag gcaaagtttt cttttctaga gccgttccca 6661 caaataatta tacgtatatg cttcttttcg tttactatat atctatattt acaagccttt 6721 attcactgat gcaatttgtt tccaaatact tttttggaga tctcataact agatatcatg 6781 atggcgcaac ttggcgctat cttaattact ctggctgcca ggcccgtgta gagggccgca 6841 agaccttctg tacgccatat agtctctaag aacttgaaca agtttctaga cctattgccg 6901 cctttcggat cgctattgtt gcggccgcca gctgaagctt cgtacgctgc aggtcgacga 6961 attctaccgt tcgtataatg tatgctatac gaagttatag atctgtttag cttgcctcgt 7021 ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg 7081 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc 7141 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg 7201 gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc 7261 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct 7321 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata 7381 aacaaccatg ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga 7441 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat 7501 ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag 7561 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc 7621 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc 7681 gatccccggc aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat 7741 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc 7801 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt 7861 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa 7921 agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc 7981 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt 8041 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc 8101 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa 8161 attgcagttt catttgatgc tcgatgagtt tttctaatca gtactgacaa taaaaagatt 8221 cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa 8281 tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag 8341 ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg 8401 ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgagctcata acttcgtata 8461 atgtatgcta tacgaacggt agaattcgaa tcagatccac tagtggccta tgcggccgcc 8521 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta 8581 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 8641 aggagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt 8701 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 8761 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 8821 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 8881 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 8941 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 9001 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 9061 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 9121 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 9181 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 9241 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 9301 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 9361 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 9421 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 9481 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 9541 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 9601 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 9661 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 9721 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 9781 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 9841 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 9901 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 9961 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 10021 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 10081 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 10141 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 10201 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 10261 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 10321 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 10381 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 10441 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 10501 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 10561 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 10621 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 10681 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg 10741 gtccttttca tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat 10801 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag 10861 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct 10921 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc 10981 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct 11041 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta 11101 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat 11161 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga 11221 tacgaggcgc gtgtaagtta caggcaagcg atccgtccta agaaaccatt attatcatga 11281 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc //