LOCUS Exported 2586 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION GoldenBraid vector pDGB2-Omega-1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRC-Delta-15 solo LTR loci. ACCESSION . VERSION . KEYWORDS YPRC-Delta-15-Omega-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2586) AUTHORS Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, Navarro-Rodriguez M, Rubio LM, Caro E TITLE Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria. JOURNAL BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 10.1186/s12896-017-0393-y. PUBMED 29132331 REFERENCE 2 (bases 1 to 2586) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2586 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(1052..1375) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS DAA" primer_bind 1057..1076 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1270..1292 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1284..1301 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1285..1301 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 1302..1358 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(1371..1387) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1371..1387) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1384..1406) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1395..1411 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1419..1449) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1464..1485 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." ORIGIN 1 gcggccgcta gggataacag ggtaatcgag atagtttgac gttcgttttt tactttgaat 61 atactcgtag tctttttact ttttgagtat aaaaaaaaaa tgactagcaa aataaaatta 121 gtagtctaaa aaagaaagct cgcactcagg atcgaactaa ggaccaacag atttgcaatc 181 tgctgcgcta ccactgcgcc atacgagctt ttgaattatg gtaattttga ttatcctaga 241 atgttatatc tcaatatctc aatatatttt ggacatctat gaaacaccca taaagcagcc 301 gctaccaaac agacaagatt cagtatgtaa ggtaaatacc tttttgcaca gttaaactac 361 ccaaacttat taaagcttga taaattactg aaattccacc tttcagttag attcaggcct 421 catatagatt agatataggg tacgtaacat tctgtcaacc aagttgttgg aatgaaagtc 481 taaaatgtca tctattcggt agcactcatg ttactagtat actgtcacat gcggtgtaac 541 gtggggacat aaaacagaca tcaaatataa tggaagctga aatgcaaaga tcgataatgt 601 aataggaatg aaacatataa aacgaaagga gaagtaatgg taatattagt atgtagaaat 661 accgattcaa ttttggggat tcttatattc tcgagagaat ttctagtata atctgtatac 721 ataatattat aggctttacc aacaatggaa tttcgacaat tatcatatta ttcaccaatt 781 aatcacaagt tggtaatgag tttgataaca agttactttc ttaacaacgt tagtatcgtc 841 aaaacactcg gttttactcg agcttgtagc acaataatac cgtgtagagt tctgtattgt 901 tcttcttagt gcttgtatat gctcatcccg accttccatt ggatccggtc tcaggagtga 961 gacgcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 1021 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 1081 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 1141 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 1201 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 1261 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc 1321 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg 1381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 1441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 1501 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc gtctcacgct gtcatgagac 1561 cggatccttt gcgaaaccct atgctctgtt gttcggattt gaaattttaa aactacatta 1621 atgtgttagt ttttctttct ttctttcttt gtcttgacgt gatttggact tctgtcttgc 1681 attcgcgtcc attcatctga cccaatattc cttttggttt tgttatcctt ataaaaagaa 1741 aggaagcttc ttagagggaa aaaaatgatg aagagtaatg ccaaaatata aataaataaa 1801 taaatatgaa aatcattttc tatttttaat agaataagaa gagcatctta agattacaat 1861 ttcaagaaat agtttacaca gtatatccaa taactccaat aaactacttt cctatacaaa 1921 tttctatggt gggattaata gtaaaacttc tgtacttctc taattcacca agaaattaag 1981 gtaaacatct ggtaagcact atccagcttt ttgctattac acatatggct tttctgcaat 2041 catttcttcc cattttgtct caagccgtta gtcttgaaac cacaggcgga gtagagttac 2101 ttgatgcggt attttacatg ccttttttca ctgcaaaaaa aatgaaatac atatttacac 2161 gatttgcagg acagtttacg atagtgagta tgcagaatag ttaacacctt tgttttatcc 2221 ttttgtgtct taattatatg atataaaggc gcctggcgtt atcggatagt aatagatgct 2281 agttatcaac atttcacaat tgaaggaaat aaagttgaag tactcaacaa aaacttactt 2341 cagaattaaa tttttggggg gaacataggc atcctatgac aggtgaccac aagcccctca 2401 acgcaatcta atattttaca aagtggtaaa attctttcgt tcttcgtttt aatatacagt 2461 catttattga ttctattaca ttaatattcc tacgcttcgg ctcacataat taacaggact 2521 tcgagtccgt taaacttggg atcaactaat ttctacggat tagggataac agggtaatgc 2581 ggccgc //