LOCUS       Exported                1116 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  Mammalian expression of HA-RagA.
ACCESSION   .
VERSION     .
KEYWORDS    pRK5 HA-RagA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Shen K, Choe A, Sabatini DM
  TITLE     Intersubunit Crosstalk in the Rag GTPase Heterodimer Enables mTORC1 
            to Respond Rapidly to Amino Acid Availability.
  JOURNAL   Mol Cell. 2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 
            10.1016/j.molcel.2017.09.026.
  PUBMED    29056322
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      66..75
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aactgcactc ggttctatcg attgattccc gaccgacagt cggtctcttc accaaggcca
       61 ttcgcgccac catgggctac ccctatgatg tgcctgacta cgcagatctc aatggtggtg
      121 gtggtgggtc gacgatgcca aatacagcca tgaagaaaaa ggtgctgctg atggggaaga
      181 gcgggtcggg gaagaccagc atgaggtcga taatcttcgc caattacatt gctcgcgaca
      241 cccggcgcct gggggccacc attgacgtgg aacactccca cgtccgattc ctagggaacc
      301 tggtgctgaa cctgtgggac tgtggcggtc aggacacctt catggaaaat tacttcacca
      361 gccagcgaga caatatcttc cgtaacgtgg aagttttgat ttacgtgttt gacgtggaga
      421 gccgcgaact ggaaaaggac atgcattatt accagtcgtg tctggaggcc atcctccaga
      481 actctcctga cgccaaaatc ttctgcctgg tgcacaaaat ggatctggtt caggaggatc
      541 agcgtgacct gatttttaaa gagcgagagg aagacctgag gcgtctgtct cgcccgctgg
      601 agtgtgcttg ttttcgaacg tccatctggg atgagacgct ctacaaagcc tggtccagca
      661 tcgtctacca gctgattccc aacgttcagc agctggagat gaacctcagg aattttgccc
      721 aaatcattga ggccgatgaa gttctgctgt tcgaaagagc tacattcttg gttatttccc
      781 actaccagtg caaagagcag cgcgacgtcc accggtttga gaagatcagc aacatcatca
      841 aacagttcaa gctgagctgc agtaaattgg ccgcttcctt ccagagcatg gaagttagga
      901 attccaactt cgctgctttc atcgacatct tcacctcaaa tacgtacgtg atggtggtca
      961 tgtcagatcc gtcgatccct tctgcggcca ctctgatcaa cattcgcaat gcccggaaac
     1021 actttgagaa gctggagaga gtggatggcc ccaagcacag tctccttatg cgttgagcgg
     1081 ccgctaagta agtaaggatc cccagctgcc gccatg
//
