LOCUS Exported 5882 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotype. ACCESSION . VERSION . KEYWORDS AbVec2.0-IGHG3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5882) TITLE Immunoglobulin expression vectors JOURNAL Unpublished REFERENCE 2 (bases 1 to 5882) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5882 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 99..120 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 135..165 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 173..189 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 178..200 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 197..213 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 197..213 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" enhancer 242..621 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 622..825 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 776..796 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 822..846 /label=LNCX /note="Human CMV promoter, forward primer" promoter 1056..1074 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1056..1073 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 1087..1106 /label=pMT2-F /note="Synthetic intron, forward primer" regulatory 2410..2419 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 2423..2557 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2460..2479) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2514..2533 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 2626..2983 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2649..2669) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2834..2969 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2896..2915 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(3003..3020) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3003..3019) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3012..3034) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3232..3687 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3319..3338) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3529..3550 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3704..3723) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3823..3845 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3883..3901) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3969..4073 /gene="bla" /label=AmpR promoter CDS 4074..4934 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4292..4311) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5105..5693 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5594..5613 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5847..5864 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatccaactg gcacgacagg 61 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 181 ggataacaat ttcacacagg aaacagctat gacatgatta cgaattaatt cgagctcgcc 241 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc 301 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 361 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 421 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 481 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 541 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 601 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 661 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 721 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 781 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt 841 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga 901 tccagcctcc gcggccggga acggtgcatt ggaacgcgga ttccccgtgc caagagtgac 961 gtaagtaccg cctatagagt ctataggccc acccccttgg cttcgttaga acgcggctac 1021 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagaataaca 1081 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcac ctcggttcta 1141 tcgattgaat tccaccatgg gatggtcatg tatcatcctt tttctagtag caactgcaac 1201 cggttgacta actagctggt cgaccaaggg cccaagcgtc ttccccctgg cgccctgctc 1261 caggagcacc tctgggggca cagcggccct gggctgcctg gtcaaggact acttcccaga 1321 gccggtgacg gtgtcgtgga actcaggcgc cctgaccagc ggcgtgcaca ccttcccggc 1381 tgtcctacag tcctcaggac tctactccct cagcagcgtg gtgaccgtgc cctccagcag 1441 cttgggcacc cagacctaca cctgcaacgt gaatcacaag cccagcaaca ccaaggtgga 1501 caagagagtt gagctgaaaa caccactggg cgatacaaca cacacttgcc caagatgccc 1561 tgaaccaaaa tcttgcgaca cacctccgcc ttgtccacga tgtcctgagc caaagagttg 1621 cgatacccct cctccttgtc cccggtgtcc cgaacccaag tcctgtgata cgcctccacc 1681 atgtcccaga tgtccagcac ctgaactcct gggaggaccg tcagtcttcc tcttcccccc 1741 aaaacccaag gataccctta tgatttcccg gacccctgag gtcacgtgcg tggtggtgga 1801 cgtgagccac gaagaccccg aggtccagtt caagtggtac gtggacggcg tggaggtgca 1861 taatgccaag acaaagccgc gggaggagca gtacaacagc acgttccgtg tggtcagcgt 1921 cctcaccgtc ctgcaccagg actggctgaa cggcaaggag tacaagtgca aggtctccaa 1981 caaagccctc ccagccccca tcgagaaaac catctccaaa accaaaggac agccccgaga 2041 accacaggtg tacaccctgc ccccatcccg ggaggagatg accaagaacc aggtcagcct 2101 gacctgcctg gtcaaaggct tctaccccag cgacatcgcc gtggagtggg agagcagcgg 2161 gcagccggag aacaactaca acaccacgcc tcccatgctg gactccgacg gctccttctt 2221 cctctacagc aagctcaccg tggacaagag caggtggcag caggggaaca tcttctcatg 2281 ctccgtgatg catgaggctc tgcacaaccg cttcacgcag aagagcctct ccctgtctcc 2341 gggtaaatga gtgcgacggc cggcaagccc ccgctccccg ggctctcggg gtcgcgcgag 2401 gaaagcttgg ccgccatggc ccaacttgtt tattgcagct tataatggtt acaaataaag 2461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 2521 gtccaaactc atcaatgtat cttatcatgt ctggatcggg aattaattcg gcgcagcacc 2581 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa 2641 ccatctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 2701 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 2761 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 2821 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 2881 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 2941 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctgt taacagcttg 3001 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 3061 cgccttgcag cacatccccc tttcgccagt tggcgtaata gcgaagaggc ccgcaccgat 3121 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc 3181 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct 3241 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 3301 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 3361 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 3421 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct 3481 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3541 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt 3601 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3661 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg 3721 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 3781 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 3841 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat 3901 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 3961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 4021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 4081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 4141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 4201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 4261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 4321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 4381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 4441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 4501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 4561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 4621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 4681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 4741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 4801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 4861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 4921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 4981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 5041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 5101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 5161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 5221 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 5281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 5341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 5401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 5461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 5521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 5581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 5641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 5701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 5761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 5821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 5881 ca //