LOCUS Exported 10279 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Direct reprogramming of adult fibroblasts to neurons. ACCESSION . VERSION . KEYWORDS U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10279) AUTHORS Drouin-Ouellet J, Lau S, Brattas PL, Rylander Ottosson D, Pircs K, Grassi DA, Collins LM, Vuono R, Andersson Sjoland A, Westergren-Thorsson G, Graff C, Minthon L, Toresson H, Barker RA, Jakobsson J, Parmar M TITLE REST suppression mediates neural conversion of adult human fibroblasts via microRNA-dependent and -independent pathways. JOURNAL EMBO Mol Med. 2017 Aug;9(8):1117-1131. doi: 10.15252/emmm.201607471. PUBMED 28646119 REFERENCE 2 (bases 1 to 10279) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10279 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(350..369) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1652..1671 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1905..1922 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2118..2140 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2137..2153 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2172..2192 /label=T3 /note="T3 promoter, forward primer" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" enhancer 2293..2672 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2673..2876 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2827..2847 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 2891..3071 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 3115..3240 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3733..3966 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 4151..4195 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 4496..4736 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" promoter 4838..5078 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" promoter 5184..5694 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" regulatory 5713..5722 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter 7126..7636 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" regulatory 7655..7664 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(8442..8462) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8860..8871) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9020..9253 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 9325..9446 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9362..9381) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9416..9435 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 9465..9600 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9527..9546 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(9632..9651) /label=T7 /note="T7 promoter, forward primer" promoter complement(9633..9651) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9661..9678) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9661..9677) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9670..9692) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9822..10277 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9909..9928) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10119..10140 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc 2221 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat 2281 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat 2341 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg 2401 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa 2461 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 2521 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta 2581 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt 2641 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg 2701 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg 2761 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc 2821 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt 2881 tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 2941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 3001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 3061 aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc agagctctct 3121 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 3181 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 3241 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 3301 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 3361 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 3421 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 3481 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 3541 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg 3601 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat 3661 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag 3721 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg 3781 cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca 3841 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg 3901 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca 3961 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa 4021 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg 4081 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa 4141 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa 4201 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg 4261 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg 4321 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga 4381 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg 4441 atctcgacgg tatcggttaa cacgagacta gcctcgagcg gccgccccct tcaccgaggg 4501 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat 4561 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta 4621 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct 4681 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa 4741 caccggtact tcaataagga cttgctccac acgtgaggag caagtcctta ttgaagtttt 4801 ttgaattctc gaccggatcc cggccgcccc cttcaccgag ggcctatttc ccatgattcc 4861 ttcatatttg catatacgat acaaggctgt tagagagata attggaatta atttgactgt 4921 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt 4981 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta 5041 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggta gtacacatta 5101 accaaatggc acacgtgagc catttggtta atgtgtactt ttttgaattc tcgacctcga 5161 gaagcttgat atcgaattcc cacggggttg gggttgcgcc ttttccaagg cagccctggg 5221 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct 5281 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt 5341 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc 5401 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga 5461 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc 5521 tcagcggggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg 5581 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag 5641 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat 5701 ccaccggtgc tagccaccat ggcgaccgca gcgtctaacc actacagcct gctcacctcc 5761 agcgcctcca tcgtacatgc cgagccgcct ggcggcatgc agcagggcgc agggggctac 5821 cgcgaggcgc agagcctggt gcagggcgac tacggcgcgc tgcagagcaa cgggcacccg 5881 ctcagccacg ctcaccagtg gatcaccgcg ctgtcccacg gcggcggcgg cgggggcggc 5941 ggcggcggtg gaggaggcgg gggaggcggc gggggaggcg gcgacggctc cccgtggtcc 6001 accagccccc taggccagcc ggacatcaag ccctcggtgg tggtacagca gggtggccga 6061 ggcgacgagc tgcacgggcc aggagcgctg cagcaacagc atcaacagca acagcaacag 6121 cagcagcagc agcagcagca gcagcagcag caacagcagc agcaacaaca gcgaccgcca 6181 catctggtgc accacgctgc caaccaccat cccgggcccg gggcatggcg gagtgcggcg 6241 gctgcagctc acctccctcc ctccatggga gcttccaacg gcggtttgct ctattcgcag 6301 ccgagcttca cggtgaacgg catgctgggc gcaggagggc agccggctgg gctgcaccac 6361 cacggcctga gggacgccca cgatgagcca caccatgcag accaccaccc gcatccgcac 6421 tctcacccac accagcaacc gcccccgcca cctcccccac aaggcccacc gggccaccca 6481 ggcgcgcacc acgacccgca ctcggacgag gacacgccga cctcagacga cctggagcag 6541 ttcgccaagc aattcaagca gaggcggatc aaactcggat ttactcaagc agacgtgggg 6601 ctggcgcttg gcaccctgta cggcaacgtg ttctcgcaga ccaccatctg caggtttgag 6661 gccctgcagc tgagcttcaa gaacatgtgc aagctgaagc ctttgttgaa caagtggttg 6721 gaagaggcag actcatcctc gggcagcccc accagcatag acaagatcgc agcgcaaggg 6781 cgcaaacgga aaaagcggac ctccatcgag gtgagcgtca agggggctct ggagagccat 6841 ttcctcaaat gccctaagcc ctcggcccag gagatcacct ccctcgcgga cagcttacag 6901 ctggagaagg aggtggtgag agtttggttt tgtaacagga gacagaaaga gaaaaggatg 6961 acccctcccg gagggactct gccgggcgcc gaggatgtgt atgggggtag tagggacacg 7021 ccaccacacc acggggtgca gacgcccgtc cagtgagcta ggaataaggg cgaattcgat 7081 atcaagctta tcgataactc gacaagcttg atatcgaatt cccacggggt tggggttgcg 7141 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg 7201 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac 7261 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag 7321 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct 7381 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg 7441 ggctgtggcc aatagcggct gctcagcggg gcgcgccgag agcagcggcc gggaaggggc 7501 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc 7561 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac 7621 cgacctctct ccccaggggg atccaccggt gctagccacc atggagagct ctggcaagat 7681 ggagagtgga gccggccagc agccgcagcc cccgcagccc ttcctgcctc ccgcagcctg 7741 cttctttgcg accgcggcgg cggcggcagc ggcggcggcc gcggcagctc agagcgcgca 7801 gcagcaacag ccgcaggcgc cgccgcagca ggcgccgcag ctgagcccgg tggccgacag 7861 ccagccctca gggggcggtc acaagtcagc ggccaagcag gtcaagcgcc agcgctcgtc 7921 ctctccggaa ctgatgcgct gcaaacgccg gctcaacttc agcggcttcg gctacagcct 7981 gccacagcag cagccggccg ccgtggcgcg ccgcaacgag cgcgagcgca accgggtcaa 8041 gttggtcaac ctgggttttg ccaccctccg ggagcatgtc cccaacggcg cggccaacaa 8101 gaagatgagc aaggtggaga cgctgcgctc ggcggtcgag tacatccgcg cgctgcagca 8161 gctgctggac gagcacgacg cggtgagcgc tgcctttcag gcgggcgtcc tgtcgcccac 8221 catctccccc aactactcca acgacttgaa ctctatggcg ggttctccgg tctcgtccta 8281 ctcctccgac gagggatcct acgaccctct tagcccagag gaacaagagc tgctggactt 8341 taccaactgg ttctgagaat tcgatatcaa gcttatcgat aagtcgacaa tcaacctctg 8401 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 8461 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 8521 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 8581 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 8641 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 8701 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 8761 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 8821 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 8881 cttccttccc gcggcctgct gccggctcta gagcctcttc cgcgtcttcg ccttcccggg 8941 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 9001 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt 9061 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 9121 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 9181 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 9241 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 9301 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 9361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 9421 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 9481 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 9541 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 9601 taggcttttg cgtcgagacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc 9661 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 9721 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 9781 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc 9841 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 9901 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 9961 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 10021 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 10081 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 10141 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 10201 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 10261 attaacgttt acaatttcc //