LOCUS Exported 9099 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses BE3-Gam in mammalian cells. ACCESSION . VERSION . KEYWORDS BE3-Gam SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9099) AUTHORS Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR TITLE Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. JOURNAL Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. PUBMED 28875174 REFERENCE 2 (bases 1 to 9099) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9099 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 132..335 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 286..306 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 377..396 /label=T7 /note="T7 promoter, forward primer" promoter 377..395 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 403..412 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 979..1662 /codon_start=1 /product="cytidine deaminase (C to U editing enzyme) from rat" /label=APOBEC-1 /note="can use ssDNA as a substrate (Komor et al., 2016)" /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL WATGLK" CDS 1711..5811 /codon_start=1 /product="nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9(D10A) /note="generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5824..6072 /codon_start=1 /product="uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995)" /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 6085..6105 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6114..6131 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(6154..6171) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6160..6384 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(6455..6471) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6455..6471) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6468..6490) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6479..6495 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6503..6533) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6548..6569 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6686..6703) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6857..7445) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6937..6956) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7616..8476) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8239..8258 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8477..8581) /gene="bla" /label=AmpR promoter primer_bind complement(8660..8679) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 8851..131 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggctaaacca 421 gcaaaacgta tcaagagtgc cgcagcggct tatgtgccac aaaaccgcga tgcggtgatt 481 accgatatta aacgcatcgg ggatttacag cgcgaagcat cacgtctgga aacggaaatg 541 aatgatgcca tcgcggaaat tacggagaaa tttgcggccc ggattgcacc gattaaaacc 601 gatattgaaa ccctttcaaa aggcgttcag ggatggtgtg aagcgaaccg cgacgaactg 661 acgaacggcg gcaaagtgaa gacggcgaat cttgtcaccg gtgatgtatc gtggcgggtc 721 cgtccaccat cagtaagtat tcgtggtatg gatgcagtga tggaaacgct ggagcgtctt 781 ggcctgcaac gctttattcg cacgaagcag gaaatcaaca aggaagcgat tttactggaa 841 ccgaaagcgg tcgcaggcgt tgccggaatt acagttaaat caggcattga ggatttttct 901 attattccat ttgaacagga agccggtatt agcggcagcg agactcccgg gacctcagag 961 tccgctacac ccgaaagtag ctcagagact ggcccagtgg ctgtggaccc cacattgaga 1021 cggcggatcg agccccatga gtttgaggta ttcttcgatc cgagagagct ccgcaaggag 1081 acctgcctgc tttacgaaat taattggggg ggccggcact ccatttggcg acatacatca 1141 cagaacacta acaagcacgt cgaagtcaac ttcatcgaga agttcacgac agaaagatat 1201 ttctgtccga acacaaggtg cagcattacc tggtttctca gctggagccc atgcggcgaa 1261 tgtagtaggg ccatcactga attcctgtca aggtatcccc acgtcactct gtttatttac 1321 atcgcaaggc tgtaccacca cgctgacccc cgcaatcgac aaggcctgcg ggatttgatc 1381 tcttcaggtg tgactatcca aattatgact gagcaggagt caggatactg ctggagaaac 1441 tttgtgaatt atagcccgag taatgaagcc cactggccta ggtatcccca tctgtgggta 1501 cgactgtacg ttcttgaact gtactgcatc atactgggcc tgcctccttg tctcaacatt 1561 ctgagaagga agcagccaca gctgacattc tttaccatcg ctcttcagtc ttgtcattac 1621 cagcgactgc ccccacacat tctctgggcc accgggttga aaagcggcag cgagactccc 1681 gggacctcag agtccgccac acccgaaagt gataaaaagt attctattgg tttagccatc 1741 ggcactaatt ccgttggatg ggctgtcata accgatgaat acaaagtacc ttcaaagaaa 1801 tttaaggtgt tggggaacac agaccgtcat tcgattaaaa agaatcttat cggtgccctc 1861 ctattcgata gtggcgaaac ggcagaggcg actcgcctga aacgaaccgc tcggagaagg 1921 tatacacgtc gcaagaaccg aatatgttac ttacaagaaa tttttagcaa tgagatggcc 1981 aaagttgacg attctttctt tcaccgtttg gaagagtcct tccttgtcga agaggacaag 2041 aaacatgaac ggcaccccat ctttggaaac atagtagatg aggtggcata tcatgaaaag 2101 tacccaacga tttatcacct cagaaaaaag ctagttgact caactgataa agcggacctg 2161 aggttaatct acttggctct tgcccatatg ataaagttcc gtgggcactt tctcattgag 2221 ggtgatctaa atccggacaa ctcggatgtc gacaaactgt tcatccagtt agtacaaacc 2281 tataatcagt tgtttgaaga gaaccctata aatgcaagtg gcgtggatgc gaaggctatt 2341 cttagcgccc gcctctctaa atcccgacgg ctagaaaacc tgatcgcaca attacccgga 2401 gagaagaaaa atgggttgtt cggtaacctt atagcgctct cactaggcct gacaccaaat 2461 tttaagtcga acttcgactt agctgaagat gccaaattgc agcttagtaa ggacacgtac 2521 gatgacgatc tcgacaatct actggcacaa attggagatc agtatgcgga cttatttttg 2581 gctgccaaaa accttagcga tgcaatcctc ctatctgaca tactgagagt taatactgag 2641 attaccaagg cgccgttatc cgcttcaatg atcaaaaggt acgatgaaca tcaccaagac 2701 ttgacacttc tcaaggccct agtccgtcag caactgcctg agaaatataa ggaaatattc 2761 tttgatcagt cgaaaaacgg gtacgcaggt tatattgacg gcggagcgag tcaagaggaa 2821 ttctacaagt ttatcaaacc catattagag aagatggatg ggacggaaga gttgcttgta 2881 aaactcaatc gcgaagatct actgcgaaag cagcggactt tcgacaacgg tagcattcca 2941 catcaaatcc acttaggcga attgcatgct atacttagaa ggcaggagga tttttatccg 3001 ttcctcaaag acaatcgtga aaagattgag aaaatcctaa cctttcgcat accttactat 3061 gtgggacccc tggcccgagg gaactctcgg ttcgcatgga tgacaagaaa gtccgaagaa 3121 acgattactc catggaattt tgaggaagtt gtcgataaag gtgcgtcagc tcaatcgttc 3181 atcgagagga tgaccaactt tgacaagaat ttaccgaacg aaaaagtatt gcctaagcac 3241 agtttacttt acgagtattt cacagtgtac aatgaactca cgaaagttaa gtatgtcact 3301 gagggcatgc gtaaacccgc ctttctaagc ggagaacaga agaaagcaat agtagatctg 3361 ttattcaaga ccaaccgcaa agtgacagtt aagcaattga aagaggacta ctttaagaaa 3421 attgaatgct tcgattctgt cgagatctcc ggggtagaag atcgatttaa tgcgtcactt 3481 ggtacgtatc atgacctcct aaagataatt aaagataagg acttcctgga taacgaagag 3541 aatgaagata tcttagaaga tatagtgttg actcttaccc tctttgaaga tcgggaaatg 3601 attgaggaaa gactaaaaac atacgctcac ctgttcgacg ataaggttat gaaacagtta 3661 aagaggcgtc gctatacggg ctggggacga ttgtcgcgga aacttatcaa cgggataaga 3721 gacaagcaaa gtggtaaaac tattctcgat tttctaaaga gcgacggctt cgccaatagg 3781 aactttatgc agctgatcca tgatgactct ttaaccttca aagaggatat acaaaaggca 3841 caggtttccg gacaagggga ctcattgcac gaacatattg cgaatcttgc tggttcgcca 3901 gccatcaaaa agggcatact ccagacagtc aaagtagtgg atgagctagt taaggtcatg 3961 ggacgtcaca aaccggaaaa cattgtaatc gagatggcac gcgaaaatca aacgactcag 4021 aaggggcaaa aaaacagtcg agagcggatg aagagaatag aagagggtat taaagaactg 4081 ggcagccaga tcttaaagga gcatcctgtg gaaaataccc aattgcagaa cgagaaactt 4141 tacctctatt acctacaaaa tggaagggac atgtatgttg atcaggaact ggacataaac 4201 cgtttatctg attacgacgt cgatcacatt gtaccccaat cctttttgaa ggacgattca 4261 atcgacaata aagtgcttac acgctcggat aagaaccgag ggaaaagtga caatgttcca 4321 agcgaggaag tcgtaaagaa aatgaagaac tattggcggc agctcctaaa tgcgaaactg 4381 ataacgcaaa gaaagttcga taacttaact aaagctgaga ggggtggctt gtctgaactt 4441 gacaaggccg gatttattaa acgtcagctc gtggaaaccc gccaaatcac aaagcatgtt 4501 gcacagatac tagattcccg aatgaatacg aaatacgacg agaacgataa gctgattcgg 4561 gaagtcaaag taatcacttt aaagtcaaaa ttggtgtcgg acttcagaaa ggattttcaa 4621 ttctataaag ttagggagat aaataactac caccatgcgc acgacgctta tcttaatgcc 4681 gtcgtaggga ccgcactcat taagaaatac ccgaagctag aaagtgagtt tgtgtatggt 4741 gattacaaag tttatgacgt ccgtaagatg atcgcgaaaa gcgaacagga gataggcaag 4801 gctacagcca aatacttctt ttattctaac attatgaatt tctttaagac ggaaatcact 4861 ctggcaaacg gagagatacg caaacgacct ttaattgaaa ccaatgggga gacaggtgaa 4921 atcgtatggg ataagggccg ggacttcgcg acggtgagaa aagttttgtc catgccccaa 4981 gtcaacatag taaagaaaac tgaggtgcag accggagggt tttcaaagga atcgattctt 5041 ccaaaaagga atagtgataa gctcatcgct cgtaaaaagg actgggaccc gaaaaagtac 5101 ggtggcttcg atagccctac agttgcctat tctgtcctag tagtggcaaa agttgagaag 5161 ggaaaatcca agaaactgaa gtcagtcaaa gaattattgg ggataacgat tatggagcgc 5221 tcgtcttttg aaaagaaccc catcgacttc cttgaggcga aaggttacaa ggaagtaaaa 5281 aaggatctca taattaaact accaaagtat agtctgtttg agttagaaaa tggccgaaaa 5341 cggatgttgg ctagcgccgg agagcttcaa aaggggaacg aactcgcact accgtctaaa 5401 tacgtgaatt tcctgtattt agcgtcccat tacgagaagt tgaaaggttc acctgaagat 5461 aacgaacaga agcaactttt tgttgagcag cacaaacatt atctcgacga aatcatagag 5521 caaatttcgg aattcagtaa gagagtcatc ctagctgatg ccaatctgga caaagtatta 5581 agcgcataca acaagcacag ggataaaccc atacgtgagc aggcggaaaa tattatccat 5641 ttgtttactc ttaccaacct cggcgctcca gccgcattca agtattttga cacaacgata 5701 gatcgcaaac gatacacttc taccaaggag gtgctagacg cgacactgat tcaccaatcc 5761 atcacgggat tatatgaaac tcggatagat ttgtcacagc ttgggggtga ctctggtggt 5821 tctactaatc tgtcagatat tattgaaaag gagaccggta agcaactggt tatccaggaa 5881 tccatcctca tgctcccaga ggaggtggaa gaagtcattg ggaacaagcc ggaaagcgat 5941 atactcgtgc acaccgccta cgacgagagc accgacgaga atgtcatgct tctgactagc 6001 gacgcccctg aatacaagcc ttgggctctg gtcatacagg atagcaacgg tgagaacaag 6061 attaagatgc tctctggtgg ttctcccaag aagaagagga aagtctaacc ggtcatcatc 6121 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 6181 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 6241 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 6301 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 6361 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac 6421 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 6481 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg 6541 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6601 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 6661 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 6721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 6781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 6841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 6901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 6961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 7321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7381 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 7441 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 7501 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 7561 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 7621 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 7681 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 7741 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 7801 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 7861 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 7921 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 7981 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 8041 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 8101 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 8161 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 8221 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 8281 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 8341 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 8401 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 8461 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 8521 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 8581 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc 8641 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc 8701 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa 8761 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc 8821 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt 8881 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 8941 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 9001 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 9061 tacggtaaac tgcccacttg gcagtacatc aagtgtatc //