LOCUS Exported 2935 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION EMMA toolkit part-entry vector position 16. ACCESSION . VERSION . KEYWORDS YCe1486 HC_Kan_RFP-p16 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2935) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 2935) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2935 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 20..41 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" protein_bind 142..163 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 178..208 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 216..232 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 250..261 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 268..945 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 990..1061 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1077..1104 /label=T7Te terminator /note="phage T7 early transcription terminator" misc_feature 1111..1131 /label=BioBrick suffix /note="universal suffix for all parts" terminator 1215..1246 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 1247..1349 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1350..2165 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1423..1442) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 2186..2213 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(2225..2812) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2305..2324) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2834..2863 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 acgtctcgcc ctagagaccg aattcgcggc cgcttctaga gcaatacgca aaccgcctct 61 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 121 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 181 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 241 atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc 301 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt 361 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt 421 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct 481 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc 541 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc 601 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc 661 gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac 721 ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt 781 cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt 841 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt 901 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc 961 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 1021 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 1081 caccttcggg tgggcctttc tgcgtttata tactagtagc ggccgctgca gggtctctgc 1141 tccgagacga aacgaattct ctagatatcg ctcaatactg accatttaaa tcatacctga 1201 cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa 1261 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat 1321 cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct 1381 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg 1441 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag 1501 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca 1561 ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc 1621 ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc caggtattag 1681 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt 1741 tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc 1801 aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta 1861 atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg 1921 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat 1981 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca 2041 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat 2101 atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt 2161 tctgagggcc caaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg 2221 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga 2281 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 2341 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 2401 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 2461 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 2521 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 2581 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 2641 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 2701 ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 2761 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 2821 tttgattttc taccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgcagt 2881 ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat tcgtt //