LOCUS Exported 2934 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION EMMA toolkit part-entry vector position 19. ACCESSION . VERSION . KEYWORDS YCe1491 HC_Kan_RFP-p19 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2934) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 2934) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2934 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 20..41 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" protein_bind 142..163 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 178..208 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 216..232 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 250..261 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 268..945 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 990..1061 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1077..1104 /label=T7Te terminator /note="phage T7 early transcription terminator" misc_feature 1111..1131 /label=BioBrick suffix /note="universal suffix for all parts" terminator 1214..1245 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 1246..1348 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1349..2164 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1422..1441) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 2185..2212 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(2224..2811) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2304..2323) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2833..2862 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 acgtctcggt gcagagaccg aattcgcggc cgcttctaga gcaatacgca aaccgcctct 61 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 121 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 181 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 241 atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc 301 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt 361 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt 421 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct 481 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc 541 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc 601 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc 661 gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac 721 ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt 781 cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt 841 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt 901 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc 961 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 1021 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 1081 caccttcggg tgggcctttc tgcgtttata tactagtagc ggccgctgca gggtctctag 1141 cgcgagacgg acgaattctc tagatatcgc tcaatactga ccatttaaat catacctgac 1201 ctccatagca gaaagtcaaa agcctccgac cggaggcttt tgacttgatc ggcacgtaag 1261 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc 1321 gagattttca ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctc 1381 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 1441 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga 1501 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 1561 gctaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 1621 tgatgatgca tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga 1681 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 1741 gcattcgatt cctgtttgta attgtccttt taacggcgat cgcgtatttc gtctcgctca 1801 ggcgcaatca cgaatgaata acggtttggt tggtgcgagt gattttgatg acgagcgtaa 1861 tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga 1921 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 1981 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 2041 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 2101 tggtattgat aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt 2161 ctgagggccc aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc 2221 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag 2281 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 2341 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 2401 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 2461 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 2521 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 2581 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 2641 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 2701 tacctcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 2761 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 2821 ttgattttct accgaagaaa ggcccacccg tgaaggtgag ccagtgagtt gattgcagtc 2881 cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt cgtt //