LOCUS Exported 1896 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION EMMA toolkit Assembly connector H-K. ACCESSION . VERSION . KEYWORDS YCe1921 HC_Kan_conn_H-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1896) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 1896) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1896 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 20..51 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 52..154 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 155..970 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(228..247) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 991..1018 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1030..1617) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1110..1129) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1639..1668 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca 61 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga 121 gttatcgaga ttttcaggag ctaaggaagc taaaatgagc catattcaac gggaaacgtc 181 ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc 241 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc 301 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat 361 ggtcaggcta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg 421 tactcctgat gatgcatggt tactcaccac tgcgatccca gggaaaacag cattccaggt 481 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg 541 ccggttgcat tcgattcctg tttgtaattg tccttttaac ggcgatcgcg tatttcgtct 601 cgctcaggcg caatcacgaa tgaataacgg tttggttggt gcgagtgatt ttgatgacga 661 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc 721 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg 781 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct 841 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca 901 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcacttga tgctcgatga 961 gtttttctga gggcccaaat gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt 1021 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gatgctcaag 1081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 1141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 1201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 1261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 1321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 1381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 1441 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 1501 gccagttacc tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 1561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 1621 gatcctttga ttttctaccg aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt 1681 gcagtccagt tacgctggag tctgaggctc gtcctgaatg atatcaagct tgaattcgtt 1741 gttatgctcg agggtgagta gcgtctcgta cccgaggata tcacctgtag ggatgttcta 1801 gagtccggaa gctgttatta gccttgagac ggtgactaga tcgtggatgc agacgaattc 1861 tctagatatc gctcaatact gaccatttaa atcata //