LOCUS Exported 1857 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION EMMA toolkit Assembly connector H-Ha. ACCESSION . VERSION . KEYWORDS YCe1913 HC_Kan_conn_H-Ha SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1857) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 1857) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1857 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 27..58 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 59..161 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 162..977 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(235..254) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 998..1025 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1037..1624) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1117..1136) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1646..1675 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 aatcatacct gacctccata gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg 61 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat 121 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatgagccat attcaacggg 181 aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata 241 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc 301 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag 361 atgagatggt caggctaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt 421 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatcccaggg aaaacagcat 481 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt 541 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacggc gatcgcgtat 601 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg agtgattttg 661 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc 721 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg 781 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc 841 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc 901 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt cacttgatgc 961 tcgatgagtt tttctgaggg cccaaatgta atcacctggc tcaccttcgg gtgggccttt 1021 ctgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgat 1081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 1141 gaagctccct cgtgcgctct cctgttccga ccctgtcgct taccggatac ctgtccgcct 1201 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 1261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 1321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 1381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 1441 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 1501 tgctgaagcc agttacctcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 1561 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 1621 tcaagaagat cctttgattt tctaccgaag aaaggcccac ccgtgaaggt gagccagtga 1681 gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata tcaagcttga 1741 attcgtttcg tctcgggatc gaggatatca cctgtaggga tgttctagag tccggaagct 1801 gttattagcc tcgagacgtg acgaattctc tagatatcgc tcaatactga ccattta //