LOCUS Exported 1849 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION EMMA toolkit Assembly connector J-K. ACCESSION . VERSION . KEYWORDS YCe2022 HC_Kan_conn_J-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1849) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 1849) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1849 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(22..41) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" terminator 130..161 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 162..264 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 265..1080 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(338..357) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 1101..1128 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1140..1727) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1220..1239) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1749..1778 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 acgtctcgtg ctgaggctaa ctgaaacacg gaaggagaca atacaggaag gaacggtacg 61 agacggacga attctctaga tatcgctcaa tactgaccat ttaaatcata cctgacctcc 121 atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca cgtaagaggt 181 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga 241 ttttcaggag ctaaggaagc taaaatgagc catattcaac gggaaacgtc ttgctcgagg 301 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 361 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg 421 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcaggcta 481 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat 541 gatgcatggt tactcaccac tgcgatccca gggaaaacag cattccaggt attagaagaa 601 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 661 tcgattcctg tttgtaattg tccttttaac ggcgatcgcg tatttcgtct cgctcaggcg 721 caatcacgaa tgaataacgg tttggttggt gcgagtgatt ttgatgacga gcgtaatggc 781 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca 841 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 901 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 961 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 1021 attgataatc ctgatatgaa taaattgcag tttcacttga tgctcgatga gtttttctga 1081 gggcccaaat gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt gctggcgttt 1141 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gatgctcaag tcagaggtgg 1201 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 1261 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 1321 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 1381 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 1441 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 1501 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 1561 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 1621 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 1681 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 1741 ttttctaccg aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt 1801 tacgctggag tctgaggctc gtcctgaatg atatcaagct tgaattcgt //