LOCUS Exported 1921 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION EMMA toolkit Assembly connector M-R. ACCESSION . VERSION . KEYWORDS YCe3733 HC_Kan_conn_M-R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1921) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 1921) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1921 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 266..297 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 298..400 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 401..1216 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(474..493) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 1237..1264 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1276..1863) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1356..1375) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1885..1914 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat 61 tcgtttttaa cgcagccgtc tcctcacgac ataatccacc atcaacatgc aagtccagtc 121 aacaactttc cctcagacgt tagtggtaat cgaacctcaa cacctagccc agaacaagcc 181 ggttgagacg aggccgttta tgacgaattc tctagatatc gctcaatact gaccatttaa 241 atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga 301 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt 361 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga 421 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 481 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc 541 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 601 tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 661 tatccgtact cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt 721 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 781 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt 841 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga 901 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc 961 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 1021 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 1081 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 1141 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct 1201 cgatgagttt ttctgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc 1261 tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg 1321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 1381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 1441 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 1501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 1561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 1621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 1681 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 1741 gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 1801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 1861 caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag 1921 t //