LOCUS       Exported                2136 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  part designed to occupy position 21 of EMMA. Functional category: 
            Dimerization domain.
ACCESSION   .
VERSION     .
KEYWORDS    YCe1905 HC_Kan_DmrA_p21
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      22..53
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        54..156
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             157..972
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(230..249)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      993..1020
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1032..1619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1112..1131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1641..1670
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(1758..2081)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 tacctgacct ccatagcaga aagtcaaaag cctccgaccg gaggcttttg acttgatcgg
       61 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
      121 gagttatcga gattttcagg agctaaggaa gctaaaatga gccatattca acgggaaacg
      181 tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
      241 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat
      301 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
      361 atggtcaggc taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
      421 cgtactcctg atgatgcatg gttactcacc actgcgatcc cagggaaaac agcattccag
      481 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
      541 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acggcgatcg cgtatttcgt
      601 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg gtgcgagtga ttttgatgac
      661 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc
      721 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
      781 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
      841 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
      901 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcactt gatgctcgat
      961 gagtttttct gagggcccaa atgtaatcac ctggctcacc ttcgggtggg cctttctgcg
     1021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgatgctca
     1081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1381 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1441 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1501 aagccagtta cctcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1561 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1621 aagatccttt gattttctac cgaagaaagg cccacccgtg aaggtgagcc agtgagttga
     1681 ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa tgatatcaag cttgaattcg
     1741 tttcgtctcg caacttatta ttccagtttt agaagctcca catcgaagac gagagtggca
     1801 tgtggtggga tgatgcctgg gtgcccagtg gcaccatagg cataatctgg agatatagtc
     1861 agtttggctc tctgacccac actcatctgg gcaacccctt cttcccagcc tcggatcacc
     1921 tcctgcttgc ctagcataaa cttaaagggc ttgtttctgt cccgggagga atcaaatttc
     1981 tttccatctt caagcatccc ggtgtagtgc accacgcagg tctggccgcg cttggggaag
     2041 gtgcgcccgt cgcctgggga gatggtttcc acctgcactc ctccacgaga cgtgacgaat
     2101 tctctagata tcgctcaata ctgaccattt aaatca
//
