LOCUS Exported 1948 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION part designed to occupy position 22 of EMMA. Functional category: Insulator. ACCESSION . VERSION . KEYWORDS YCe1911 HC_Kan_Insulator_FB_p2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1948) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 1948) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1948 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 292..323 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 324..426 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 427..1242 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(500..519) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 1263..1290 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1302..1889) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1382..1401) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1911..1940 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt 61 cgttacgtct cgatggggcc gcgaattctg aaagacccca cctgtaggtt tggcaagccc 121 agggatgtac gtccctaacc cgctaggggg cagcaactag tcccaggcct gcactgccgc 181 ctgccggcag gggtccagtc gctagcgcat gcctgcagac tcgagacgac gaattctcta 241 gatatcgctc aatactgacc atttaaatca tacctgacct ccatagcaga aagtcaaaag 301 cctccgaccg gaggcttttg acttgatcgg cacgtaagag gttccaactt tcaccataat 361 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa 421 gctaaaatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac 481 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg 541 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa 601 ggtagcgttg ccaatgatgt tacagatgag atggtcaggc taaactggct gacggaattt 661 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc 721 actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa 781 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat 841 tgtcctttta acggcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac 901 ggtttggttg gtgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc 961 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat 1021 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga 1081 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag 1141 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg 1201 aataaattgc agtttcactt gatgctcgat gagtttttct gagggcccaa atgtaatcac 1261 ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt ttttccatag gctccgcccc 1321 cctgacgagc atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc gacaggacta 1381 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 1441 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 1501 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 1561 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 1621 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 1681 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 1741 agaacagtat ttggtatctg cgctctgctg aagccagtta cctcggaaaa agagttggta 1801 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 1861 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gattttctac cgaagaaagg 1921 cccacccgtg aaggtgagcc agtgagtt //