LOCUS Exported 2420 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION part designed to occupy position 15 of EMMA. Functional category: Selection marker. ACCESSION . VERSION . KEYWORDS YCe2046 HC_Kan_PuroR_p15 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2420) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 2420) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2420 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 89..688 /codon_start=1 /gene="pac from Streptomyces" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA" primer_bind complement(89..108) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 585..605 /label=Puro-F /note="Puromycin resistance gene, forward primer" terminator 768..799 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 800..902 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 903..1718 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(976..995) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 1739..1766 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1778..2365) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1858..1877) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2387..2416 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 agttgattgc agtccagtta cgctggagtc tgaggctcgt cctgaatgat atcaagcttg 61 aattcgttac gtctcggcaa ccgccaccat gaccgagtac aagcccacgg tgcgcctcgc 121 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 181 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 241 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 301 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 361 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 421 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtgtc 481 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 541 cgagcgcgcc ggggtgcccg ccttcctgga aacctccgcg ccccgcaacc tccccttcta 601 cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg 661 gtgcatgacc cgcaagcccg gtgcctaata accctcgaga cgaaacgaat tctctagata 721 tcgctcaata ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc 781 cgaccggagg cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa 841 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta 901 aaatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg 961 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa 1021 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta 1081 gcgttgccaa tgatgttaca gatgagatgg tcaggctaaa ctggctgacg gaatttatgc 1141 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg 1201 cgatcccagg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata 1261 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc 1321 cttttaacgg cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt 1381 tggttggtgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga 1441 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct 1501 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag 1561 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt 1621 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata 1681 aattgcagtt tcacttgatg ctcgatgagt ttttctgagg gcccaaatgt aatcacctgg 1741 ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt ccataggctc cgcccccctg 1801 acgagcatca caaaaatcga tgctcaagtc agaggtggcg aaacccgaca ggactataaa 1861 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 1921 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 1981 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 2041 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 2101 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 2161 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 2221 cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggaaaaagag ttggtagctc 2281 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 2341 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca 2401 cccgtgaagg tgagccagtg //