LOCUS Exported 1860 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION part designed to occupy position 8 of EMMA. Functional category: Peptide linker. ACCESSION . VERSION . KEYWORDS YCe2746 HC_Kan_Linker1_p8 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1860) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 1860) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1860 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 35..66 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 67..169 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 170..985 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(243..262) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 1006..1033 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1045..1632) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1125..1144) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1654..1683 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 accatttaaa tcatacctga cctccatagc agaaagtcaa aagcctccga ccggaggctt 61 ttgacttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc 121 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tgagccatat 181 tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata 241 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta 301 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga 361 tgttacagat gagatggtca ggctaaactg gctgacggaa tttatgcctc ttccgaccat 421 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa 481 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct 541 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacggcga 601 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag 661 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa 721 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct 781 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga 841 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca 901 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca 961 cttgatgctc gatgagtttt tctgagggcc caaatgtaat cacctggctc accttcgggt 1021 gggcctttct gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 1081 aaatcgatgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 1141 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 1201 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 1261 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 1321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 1381 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 1441 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 1501 ctgcgctctg ctgaagccag ttacctcgga aaaagagttg gtagctcttg atccggcaaa 1561 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 1621 aaaggatctc aagaagatcc tttgattttc taccgaagaa aggcccaccc gtgaaggtga 1681 gccagtgagt tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc 1741 aagcttgaat tcgtttcgtc tcgacgctcc gcttccgcca gatgcgctgc ctcctccgct 1801 gccagatctg agtccagagc ctcgagacgt gacgaattct ctagatatcg ctcaatactg //