LOCUS Exported 2160 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION part designed to occupy position 1 of EMMA. Functional category: Inverted Terminal Repeat. ACCESSION . VERSION . KEYWORDS YCe2748 HC_Kan_5'ITR-PB_p1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2160) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 2160) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2160 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 103..137 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" terminator 506..537 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 538..640 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 641..1456 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(714..733) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 1477..1504 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1516..2103) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1596..1615) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2125..2154 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat caagcttgaa 61 ttcgttacgt ctcgtaggac tatcctaacg tccacttctt aaccctagaa agatagtctg 121 cgtaaaattg acgcatgcat tcttgaaata ttgctctctc tttctaaata gcgcgaatcc 181 gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc 241 aatgcggtaa gtgtcactga ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg 301 attatctttt acgtgacttt taagatttaa ctcatacgat aattatattg ttatttcatg 361 ttctacttac gtgataactt attatatata tattttcttg ttatagatca ggtggatgaa 421 agcgtgtaat ggcgagacga agacgaattc tctagatatc gctcaatact gaccatttaa 481 atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga 541 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt 601 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga 661 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 721 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc 781 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 841 tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 901 tatccgtact cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt 961 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 1021 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt 1081 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga 1141 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc 1201 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 1261 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 1321 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 1381 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct 1441 cgatgagttt ttctgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc 1501 tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg 1561 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 1621 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 1681 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 1741 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 1801 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 1861 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 1921 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 1981 gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 2041 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 2101 caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag //