LOCUS       Exported                2432 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  part designed to occupy position 20 of EMMA. Functional category: 
            IRES sequence.
ACCESSION   .
VERSION     .
KEYWORDS    YCe3752 HC_Kan_IRES2_p20
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    117..690
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    140..692
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(284..301)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     511..530
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     terminator      777..808
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        809..911
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             912..1727
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(985..1004)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1748..1775
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1787..2374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1867..1886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2396..2425
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgttacgtc tcgagcggct aacgctcgat aagcttgata tcgaattccg cccccccccc
      121 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
      181 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
      241 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
      301 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
      361 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
      421 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
      481 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
      541 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
      601 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
      661 gtggttttcc tttgaaaaac acgatgataa tatggcctct ggacgagacg aagacgaatt
      721 ctctagatat cgctcaatac tgaccattta aatcatacct gacctccata gcagaaagtc
      781 aaaagcctcc gaccggaggc ttttgacttg atcggcacgt aagaggttcc aactttcacc
      841 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
      901 aggaagctaa aatgagccat attcaacggg aaacgtcttg ctcgaggccg cgattaaatt
      961 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     1021 gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     1081 gcaaaggtag cgttgccaat gatgttacag atgagatggt caggctaaac tggctgacgg
     1141 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     1201 tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat cctgattcag
     1261 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     1321 gtaattgtcc ttttaacggc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     1381 ataacggttt ggttggtgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     1441 aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg
     1501 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     1561 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     1621 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     1681 atatgaataa attgcagttt cacttgatgc tcgatgagtt tttctgaggg cccaaatgta
     1741 atcacctggc tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc cataggctcc
     1801 gcccccctga cgagcatcac aaaaatcgat gctcaagtca gaggtggcga aacccgacag
     1861 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1921 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1981 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2041 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2101 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2161 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2221 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttacctcg gaaaaagagt
     2281 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2341 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgattt tctaccgaag
     2401 aaaggcccac ccgtgaaggt gagccagtga gt
//
