LOCUS Exported 10468 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms. ACCESSION . VERSION . KEYWORDS pDD287 (mKate2^SEC^3xMyc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10468) TITLE FP-SEC vectors for homologous recombination in C. elegans JOURNAL Unpublished REFERENCE 2 (bases 1 to 10468) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10468 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 117..136 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 370..387 /label=L4440 /note="L4440 vector, forward primer" protein_bind 504..525 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 540..570 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 578..594 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 583..605 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 602..618 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 602..618 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 891..1196 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" CDS complement(1241..1330) /codon_start=1 /product="3 tandem Myc epitope tags" /label=3xMyc /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" protein_bind complement(1349..1382) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind 1735..1759 /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS complement(1760..2785) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" protein_bind complement(2788..2812) /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" terminator 4730..5053 /gene="tbb-2" /label=tbb-2 terminator /note="transcription terminator for the C. elegans tubulin beta-2 gene" CDS complement(6871..6897) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind complement(6907..6940) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." CDS complement(6958..6978) /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" CDS complement(7899..8204) /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind complement(8470..8487) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8470..8486) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8479..8501) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(8695..8714) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8814..8836 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(8874..8892) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8960..9064 /gene="bla" /label=AmpR promoter CDS 9065..9925 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9283..9302) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10096..216 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 61 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 121 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 181 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 241 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 301 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 361 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 421 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 481 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 541 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 601 acaggaaaca gctatgacca tgttatcgat ttcctaggaa cccgaagtat gtcaaaaaga 661 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca 721 aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga atgaagcccg 781 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg 841 gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa atgcagttta 901 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata 961 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag 1021 ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga 1081 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca 1141 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt 1201 caggctccct tatacacagc cagtctgcag gtcgagccgg caagtcttcc tcgctgatca 1261 acttctgctc gaggtcctcc tcggagatga gcttttgctc aagatcctct tcagaaataa 1321 gtttttgttc agatccggct ccctgaaaat aacttcgtat aaagtatcct atacgaagtt 1381 attaaaaatt gcggtcataa actgaaacgt aacatatgat aaggtatttt gtgtgcggtt 1441 ttttctatga tgtttcaaaa aaatttatca gaagtaaaaa acataagaag tgtacacaga 1501 acattgtgta gattttggga tagaattatt attaatgggg ggagggagca caattttttt 1561 cgacttttta ttacgaatta attctatttt tgaatctaca caatttcatt gttagaggtg 1621 acttaaaaga agctaaaaaa caaagaaatt aagagaagtt ttttgataat aacaaaaata 1681 gggggtggga gcacagggag aaagagcatg tagggatgtt gaagagtaat tggacccact 1741 ttgtacaaga aagctgggtc tattcctttg ccctcggacg agtgctgggg cgtcggtttc 1801 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga 1861 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg 1921 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa 1981 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga 2041 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga 2101 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc 2161 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg 2221 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg 2281 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat 2341 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct 2401 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg 2461 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat 2521 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg 2581 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc 2641 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg 2701 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg 2761 tcgcggtgag ttcaggcttt ttcatggagc ctgctttttt gtacaaactt gcggcaccgc 2821 ctgacatatt accttaaaat tcaaaaatta atttcagatc agtcataacc aagaaaacaa 2881 aataaacgtt aggacagaaa attcaaatta attaaaaatt atgctgaata agaaaattac 2941 atgcaaagcc ttattttcct catagaaaca gttgaataac atatttttta attgaaaaca 3001 tagcaaaatt cgtggtaaaa tttgtcaaaa ccttggaaaa aatcgaaaaa agaacatttc 3061 ccgatgcatt ttcctatttc tcgttgccga caacgtataa acgttgaata ctcagtcaac 3121 gaatttggct gctggcttgg actctcgcac ggaagagacg cgtgtgtaga tttacgacga 3181 aagcaaaaat gatcaccaaa aacggaacgt tgagctggac ggaaatagtg gtaaagtgac 3241 atgattatag tttgaagatt tctaatttca caattagagc aaatgttgtt cggtatttat 3301 tttcaacggt atttatacta ttttccacct ttttctagaa cattcgagct gcttgttgca 3361 aaaggagggc gactcacatt cggtacatgg aaaagtagtg tacacaataa agagacccag 3421 atacattttc cgtctgcgtc tctttgcacc caccgggagt attttcaaac gaatgcatct 3481 aggaccttct agaacattct gtaaggctgc agaatgcggg tatataagga aagcgggctc 3541 agaggaagcc aacacgcttt gttctagtgc atctaaaaaa cttcgaaaat atgtcaaatc 3601 ttctcactgt tcatcaaaat cttccagcac ttccagttga cgcgacaagt gatgaagttc 3661 gcaagaacct catggatatg ttccgtgaca gacaagcctt ctccgagcac acctggaaga 3721 tgcttctctc agtgtgccgt tcttgggctg cctggtgcaa gcttaacaac cgcaagtggt 3781 tcccagccga gccagaagac gttcgtgact acttgctcta cctccaagct cgtggattgg 3841 cggtgaagac gatccaacaa catctcggac agctcaatat gctccatcgc cgatctggac 3901 ttccacgccc atcagattct aatgccgtat ctctcgtaat gagacgtatc cgcaaggaaa 3961 atgttgatgc tggagagaga gccaagcaag ccctcgcctt cgaaaggtaa gtttaaatat 4021 taatgtattc aatcctaaga cttgacgaaa ttttcagaac cgacttcgat caggtccgtt 4081 ctcttatgga aaattccgat cgttgccagg acatccgaaa cctcgccttc ctcggaattg 4141 cttacaacac acttctccgt atcgccgaaa ttgcccgcat tcgcgtcaag gatattagtc 4201 gcacagacgg aggaagaatg ctcatccaca tcggacgtac aaaaacgctg gtctcgactg 4261 ccggagtcga aaaagcgctc tcccttggag ttaccaagct cgttgagcgc tggatttcgg 4321 tttccggagt cgccgatgat ccaaacaact acctgttctg tcgtgttcgc aagaacggag 4381 ttgctgcccc gagtgcaaca tcacaactct cgactcgagc tcttgaaggt aagtttaaac 4441 aacatattag ctaagaataa gctgaaaata aattttcagg aatcttcgaa gctacccacc 4501 gtctcatcta cggagccaag gatgattccg gacaacgtta cttggcttgg tctggacatt 4561 ccgcccgtgt cggagctgcc cgtgacatgg ctcgcgctgg agtttccatc ccagagatta 4621 tgcaagccgg aggttggacc aacgtcaaca ttgtgatgaa ctatattaga aacttggatt 4681 ctgaaactgg cgccatggtt cgtcttctcg aagacggtga ttaagataaa tgcaaaatcc 4741 tttcaagcat tcccttcttc tctatcactc ttctttcttt ttgtcaaaaa attctctcgc 4801 taatttattt gcttttttaa tgttattatt ttatgacttt ttatagtcac tgaaaagttt 4861 gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt ctgatgtact ttcacaatct 4921 ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt tgagaaaaat gcgagcgctc 4981 aaatatttgt attgtgttcg ttgagtgacc caacaaaaag aggaaacttt attgtgccgc 5041 caagaaaaaa gtctcactga atctcaaata ttttattaga aaacaccaac aatttagata 5101 tttctgtatc cacggttggc gttgacagcc tctgatggtg gagacttgtc tgggcactgg 5161 cagtactcgg catcctttcc tggtcttccg tctggtccag tctctccctg ggttccgtct 5221 ggtccttgac gtccttgctt tccagatggt ccttctggtc caacggttcc tggctctcct 5281 ggtggtccgt gaagaccgtt ctgtccagct ggtcctgttg gtccttgatc tcctcttggt 5341 ccctttggtc ctggtcttcc gattggatgc tcggcgtcgc gtcccttctc tcctggtggt 5401 cctggctctc cgatctttcc gatttgaccg gatgatcctt gctctcctgg atgtcctggt 5461 tgtccatctc ttcctgggtt tccattttgt cctcttggtc ctggaagtcc acgtggtcct 5521 ggtcttccaa gagctcctgg tggtccaact ggtccttgtg ggcaagtgaa gcatccgaca 5581 gactcgcgtt gtggagcgat atcatcagct ccaactcctg gaacaccgtc aagtcctgga 5641 actccgtcga gtcctggaac tcctggaact ccctttggtc cggatggtcc agctgggcac 5701 ttgttatcag cattgcagtt gcaggttggt ccatttccat tggctccatt tggagtcttt 5761 ggctggttga agactggtgg aactgctggt ggttgacctg gtggagcaga tggggatcca 5821 tcagcatgtg gagttggggt agcgttggtc tcttcatatt gacaacggac acgcttgcta 5881 gatcttccga tgacaactat gtccttccac aatccattgg tagattgcta gaaaattaaa 5941 aattatttaa aattcaaaat ataatttaac tcactctgaa cacattcatc tcggtatcaa 6001 gcttctctct gaatccatca acctccgagt agatggttga catggtcatg aagcatacca 6061 taagagtggc gacagtgacc gaagccgtca cataacacgc aagttttaca gacatctctg 6121 aaattatcaa atttaggatt tcaaaataat tcggccaaag aaatgtaact cacctagtct 6181 tcgagaagca gagacttgtc gatttcctac cggattcgtc tccttttatt aggaaatctt 6241 tccgcaagtt ttcaagtttt ttttgttttt tttttacatt accgacgaga cagtgtgcga 6301 gacgcagaga cgataaaaga ctggataaga acgggagata aggagtacgg atgtaggtga 6361 ggaatgtggc cgaaaggaca gatgggcttt attcgggaag attggttggg aaatgaacca 6421 gagaagaatg actgaataaa gttgttgaaa cttggtgaga atggaagata gacaatcgag 6481 ggcagctagt tttccttcct cctctatata tttttatgtt ttcttttaaa ccttcctcca 6541 tatttctgaa ttgaatgttg ttgcatattg tgtgtcctta tttcatttcc atcatccttt 6601 tgtgtgtttc ccccttccac caattccccc cacgtcctaa tgaaatgaaa gcgagaagat 6661 aaataaactt gcacattgtg ttcagatgag aggagcggaa tacgaattgg gagacggaaa 6721 gagaaaaaaa aatgtttaga tttggattga attaattttt tagaaagcaa gaaaaaaaac 6781 acaaagagaa taaataaata ataggaaaaa aaacaaatat tcagtattta aaatttgaaa 6841 attcaacgac gttggcgtcg atcatcctta ggcgtagtct gggacgtcgt atgggtatcc 6901 ctgaaaataa cttcgtataa agtatcctat acgaagttat tttaaactta cctttccgga 6961 ttgaaagtac agattctcac ggtgtccgag cttggatggg aggtcgcagt aacgggcgac 7021 ggcgacctcg tgttgctcga cgtaggtctc cttgtcggcc tccttgatac gctcaagacg 7081 acggtcgacg tagtagactc ctggcatctt gaggttcttg gctggcttct tggaacggta 7141 ggtggtctga aaatttaaat cagattagtt agttagtaaa atcatgttta aacttacctt 7201 gaggttgcag atgaggtgtc ctcctccgac gagcttgagg gccatgtcgg cacgtccctc 7261 aagtcctccg tcggctgggt agagggtctc ggtggaggcc tcccatccga gggtcttctt 7321 ttgcatgact ggtccgttgg atgggaagtt gactccacgg atctgaaaat ttaaatatgt 7381 atggttagtt agtaccgaac tgtttaaact taccttgacg ttgtagatga ggcatccgtc 7441 ttggagggag gtgtcttggg tggcggtgag gactcctccg tcctcgtagg tggtgacacg 7501 ctcccaggtg aatccctctg ggaaggattg cttgaagaag tctgggattc cttgggtgtg 7561 gttgatgaag gtctgaaaat ttaaataatc agggttagtt agtatatata tgtttaaact 7621 taccttggat ccgtacatga aggaggtggc gaggatgtcg aaggcgaatg ggagtggtcc 7681 tccctcgacg gccttgatac gcatggtttg ggttccctcg tatggctttc cctctccctc 7741 ggaggtgcac ttgaagtggt ggttgttgac ggttccctcc atgtagagct tcatatgcat 7801 gttttcttta atgagctcgg agaccatcga tgctcctgag gctcccgatg ctccctaggt 7861 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg 7921 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata 7981 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata 8041 tgcaccaccg ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg 8101 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga 8161 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt 8221 ctcgtcggca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg 8281 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct 8341 taccgaaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca 8401 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtaacta 8461 gtgccggcga ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 8521 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 8581 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta 8641 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat 8701 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc 8761 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 8821 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt 8881 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 8941 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 9001 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 9061 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 9121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 9181 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 9241 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 9301 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 9361 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 9421 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 9481 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 9541 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 9601 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 9661 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 9721 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 9781 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 9841 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 9901 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 9961 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 10021 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 10081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 10141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 10201 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 10261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 10321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 10381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 10441 cttggagcga acgacctaca ccgaactg //