LOCUS Exported 8966 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION expresses a chimeric cMYC-activator fusion. ACCESSION . VERSION . KEYWORDS pSN27 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8966) AUTHORS Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M TITLE OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. JOURNAL Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071. PUBMED 28813671 REFERENCE 2 (bases 1 to 8966) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8966 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..511 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 430..448 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 526..1242 /codon_start=1 /product="enhanced monomeric variant of CFP (Rizzo et al., 2004)" /label=mCerulean /note=" mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(571..592) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(832..851) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1179..1200 /label=EGFP-C /note="EGFP, forward primer" CDS 1252..1308 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 1309..2625 /codon_start=1 /product="human c-Myc proto-oncogene" /label=c-Myc /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA" misc_feature 2833..3421 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2886..2906) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3304..3315) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3574..3754 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 3826..3947 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3863..3882) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3917..3936 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 3987..4122 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4049..4068 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(4142..4161) /label=T7 /note="T7 promoter, forward primer" promoter complement(4143..4161) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4171..4188) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4171..4187) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4180..4202) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4329..4784 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4416..4435) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4626..4647 /label=F1ori-F /note="F1 origin, forward primer" promoter 4810..4914 /gene="bla" /label=AmpR promoter CDS 4915..5775 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5133..5152) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5946..6534 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6435..6454 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6688..6705 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6822..6843 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6858..6888 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6896..6912 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6901..6923 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6920..6936 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6920..6936 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6955..6975 /label=T3 /note="T3 promoter, forward primer" promoter 6957..6975 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 7003..7229 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7230..7410 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7457..7582 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8075..8308 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8493..8537 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 8804..8921 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc 61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc 121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc 181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac 241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc 301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag 361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct 421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct 481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc 541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 721 acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc 781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 961 gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac 1021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 1141 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 1201 accgccgccg ggatcactct cggcatggac gagctgtaca agggatctgg agcaacaaac 1261 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat gcccctcaac 1321 gttagcttca ccaacaggaa ctatgacctc gactacgact cggtgcagcc gtatttctac 1381 tgcgacgagg aggagaactt ctaccagcag cagcagcaga gcgagctgca gcccccggcg 1441 cccagcgagg atatctggaa gaaattcgag ctgctgccca ccccgcccct gtcccctagc 1501 cgccgctccg ggctctgctc gccctcctac gttgcggtca cacccttctc ccttcgggga 1561 gacaacgacg gcggtggcgg gagcttctcc acggccgacc agctggagat ggtgaccgag 1621 ctgctgggag gagacatggt gaaccagagt ttcatctgcg acccggacga cgagaccttc 1681 atcaaaaaca tcatcatcca ggactgtatg tggagcggct tctcggccgc cgccaagctc 1741 gtctcagaga agctggcctc ctaccaggct gcgcgcaaag acagcggcag cccgaacccc 1801 gcccgcggcc acagcgtctg ctccacctcc agcttgtacc tgcaggatct gagcgccgcc 1861 gcctcagagt gcatcgaccc ctcggtggtc ttcccctacc ctctcaacga cagcagctcg 1921 cccaagtcct gcgcctcgca agactccagc gccttctctc cgtcctcgga ttctctgctc 1981 tcctcgacgg agtcctcccc gcagggcagc cccgagcccc tggtgctcca tgaggagaca 2041 ccgcccacca ccagcagcga ctctgaggag gaacaagaag atgaggaaga aatcgatgtt 2101 gtttctgtgg aaaagaggca ggctcctggc aaaaggtcag agtctggatc accttctgct 2161 ggaggccaca gcaaacctcc tcacagccca ctggtcctca agaggtgcca cgtctccaca 2221 catcagcaca actacgcagc gcctccctcc actcggaagg actatcctgc tgccaagagg 2281 gtcaagttgg acagtgtcag agtcctgaga cagatcagca acaaccgaaa atgcaccagc 2341 cccaggtcct cggacaccga ggagaatgtc aagaggcgaa cacacaacgt cttggagcgc 2401 cagaggagga acgagctaaa acggagcttt tttgccctgc gtgaccagat cccggagttg 2461 gaaaacaatg aaaaggcccc caaggtagtt atccttaaaa aagccacagc atacatcctg 2521 tccgtccaag cagaggagca aaagctcatt tctgaagagg acttgttgcg gaaacgacga 2581 gaacagttga aacacaaact tgaacagcta cggaactctt gtgcgcactt agacggcgag 2641 gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat gttgggggac 2701 ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg cgctctggat 2761 atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga cgagtacggt 2821 gggtgagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 2881 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 2941 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 3001 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 3061 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 3121 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 3181 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 3241 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 3301 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 3361 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 3421 cctggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta 3481 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 3541 atatcctgct agctccggat ccggacctgt actgggtctc tctggttaga ccagatctga 3601 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 3661 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 3721 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 3781 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 3841 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 3901 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 3961 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 4021 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 4081 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc 4141 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg 4201 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 4261 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 4321 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 4381 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 4441 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 4501 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 4561 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 4621 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 4681 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 4741 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 4801 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 4861 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 4921 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 4981 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 5041 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 5101 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 5161 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 5221 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 5281 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 5341 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 5401 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 5461 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 5521 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 5581 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 5641 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 5701 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 5761 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 5821 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 5881 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 5941 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 6001 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 6061 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 6121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 6181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 6241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 6301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 6361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 6481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 6541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 6601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 6661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 6721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 6781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 6841 cattaggcac cccaggtttt acactttatg cttccggctc gtatgttgtg tggaattgtg 6901 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 6961 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac 7021 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa 7081 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc 7141 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg 7201 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct 7261 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 7321 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 7381 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 7441 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag 7501 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga 7561 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa 7621 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag 7681 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag 7741 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt 7801 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa 7861 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc 7921 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat 7981 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa 8041 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg 8101 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat 8161 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc 8221 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg 8281 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt 8341 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga 8401 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact 8461 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag 8521 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat 8581 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta 8641 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc 8701 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca 8761 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg 8821 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 8881 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc 8941 tcgagaagct tgatatcgaa ttccac //