LOCUS       Exported                 839 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    DCT.VV.Orf236
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             341..382
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             392..409
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(432..449)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    438..662
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(795..814)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc cagctgacca ccatgtccgt catatcgaat
       61 ccatcaaagt acaaacagtc aaacacgaac aaacacatgt tagagttttt atattctttc
      121 tttttgtgta ttcctaaact tccaaacggg agcggtacat tatgttcgtc tacaagaaca
      181 atttcagaat ccaatacgat agacgtagct tttttaaatg ctttcggtat gtattctttg
      241 agataagcaa ttcccgggga tccgaataaa gggtcaagac aattctgcag atatccagca
      301 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
      361 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac
      421 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      481 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
      541 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
      601 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
      661 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
      721 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      781 cgccctagcg cccgctcctt tcgctttctt cccttctttc tcgccacgtt cgccggctt
//
