LOCUS       Exported               12800 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLenti-MetGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12800)
  TITLE     Rimm Lab Lenti MetGFP plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12800
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     36..56
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        38..56
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        84..310
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             311..491
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    538..663
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1156..1389
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1574..1618
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1885..2001
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2024..2327
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2328..2531
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2482..2502
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2528..2552
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6802..6811
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6808..7527
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6808..7524
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6853..6874)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7114..7133)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7461..7482
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7543..8131
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7596..7616)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8014..8025)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8180..8203)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8577..8596
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8665..9264
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8665..8684)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9161..9181
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9402..9635
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9707..9828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9744..9763)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9798..9817
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9868..10003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9930..9949
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(10023..10042)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10024..10042)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10052..10069)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10052..10068)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10061..10083)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10210..10665
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10297..10316)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10507..10528
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10691..10795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10796..11656
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11014..11033)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11827..12415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12316..12335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12569..12586
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12703..12724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12739..12769
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12777..12793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12782..4
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
       61 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
      121 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
      181 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
      241 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
      301 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      481 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      541 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      601 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      661 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
      721 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
      781 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
      841 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
      901 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
      961 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1021 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1081 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1141 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1201 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1261 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1321 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1381 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1441 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1501 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1561 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1621 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     1681 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     1741 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     1801 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     1861 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     1921 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     1981 aattacaaaa ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg
     2041 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2101 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2161 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2221 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     2281 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     2341 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     2401 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2461 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2521 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2581 acctccatag aagacaccga ctctagagga tccaccatga aggcccccgc tgtgcttgca
     2641 cctggcatcc tcgtgctcct gtttaccttg gtgcagagga gcaatgggga gtgtaaagag
     2701 gcactagcaa agtccgagat gaatgtgaat atgaagtatc agcttcccaa cttcaccgcg
     2761 gaaacaccca tccagaatgt cattctacat gagcatcaca ttttccttgg tgccactaac
     2821 tacatttatg ttttaaatga ggaagacctt cagaaggttg ctgagtacaa gactgggcct
     2881 gtgctggaac acccagattg tttcccatgt caggactgca gcagcaaagc caatttatca
     2941 ggaggtgttt ggaaagataa catcaacatg gctctagttg tcgacaccta ctatgatgat
     3001 caactcatta gctgtggcag cgtcaacaga gggacctgcc agcgacatgt ctttccccac
     3061 aatcatactg ctgacataca gtcggaggtt cactgcatat tctccccaca gatagaagag
     3121 cccagccagt gtcctgactg tgtggtgagc gccctgggag ccaaagtcct ttcatctgta
     3181 aaggaccggt tcatcaactt ctttgtaggc aataccataa attcttctta tttcccagat
     3241 catccattgc attcgatatc agtgagaagg ctaaaggaaa cgaaagatgg ttttatgttt
     3301 ttgacggacc agtcctacat tgatgtttta cctgagttca gagattctta ccccattaag
     3361 tatgtccatg cctttgaaag caacaatttt atttacttct tgacggtcca aagggaaact
     3421 ctagatgctc agacttttca cacaagaata atcaggttct gttccataaa ctctggattg
     3481 cattcctaca tggaaatgcc tctggagtgt attctcacag aaaagagaaa aaagagatcc
     3541 acaaagaagg aagtgtttaa tatacttcag gctgcgtatg tcagcaagcc tggggcccag
     3601 cttgctagac aaataggagc cagcctgaat gatgacattc ttttcggggt gttcgcacaa
     3661 agcaagccag attctgccga accaatggat cgatctgcca tgtgtgcatt ccctatcaaa
     3721 tatgtcaacg acttcttcaa caagatcgtc aacaaaaaca atgtgagatg tctccagcat
     3781 ttttacggac ccaatcatga gcactgcttt aataggacac ttctgagaaa ttcatcaggc
     3841 tgtgaagcgc gccgtgatga atatcgaaca gagtttacca cagctttgca gcgcgttgac
     3901 ttattcatgg gtcaattcag cgaagtcctc ttaacatcta tatccacctt cattaaagga
     3961 gacctcacca tagctaatct tgggacatca gagggtcgct tcatgcaggt tgtggtttct
     4021 cgatcaggac catcaacccc tcatgtgaat tttctcctgg actcccatcc agtgtctcca
     4081 gaagtgattg tggagcatac attaaaccaa aatggctaca cactggttat cactgggaag
     4141 aagatcacga agatcccatt gaatggcttg ggctgcagac atttccagtc ctgcagtcaa
     4201 tgcctctctg ccccaccctt tgttcagtgt ggctggtgcc acgacaaatg tgtgcgatcg
     4261 gaggaatgcc tgagcgggac atggactcaa cagatctgtc tgcctgcaat ctacaaggtt
     4321 ttcccaaata gtgcacccct tgaaggaggg acaaggctga ccatatgtgg ctgggacttt
     4381 ggatttcgga ggaataataa atttgattta aagaaaacta gagttctcct tggaaatgag
     4441 agctgcacct tgactttaag tgagagcacg atgaatacat tgaaatgcac agttggtcct
     4501 gccatgaata agcatttcaa tatgtccata attatttcaa atggccacgg gacaacacaa
     4561 tacagtacat tctcctatgt ggatcctgta ataacaagta tttcgccgaa atacggtcct
     4621 atggctggtg gcactttact tactttaact ggaaattacc taaacagtgg gaattctaga
     4681 cacatttcaa ttggtggaaa aacatgtact ttaaaaagtg tgtcaaacag tattcttgaa
     4741 tgttataccc cagcccaaac catttcaact gagtttgctg ttaaattgaa aattgactta
     4801 gccaaccgag agacaagcat cttcagttac cgtgaagatc ccattgtcta tgaaattcat
     4861 ccaaccaaat cttttattag tggtgggagc acaataacag gtgttgggaa aaacctgaat
     4921 tcagttagtg tcccgagaat ggtcataaat gtgcatgaag caggaaggaa ctttacagtg
     4981 gcatgtcaac atcgctctaa ttcagagata atctgttgta ccactccttc cctgcaacag
     5041 ctgaatctgc aactccccct gaaaaccaaa gcctttttca tgttagatgg gatcctttcc
     5101 aaatactttg atctcattta tgtacataat cctgtgttta agccttttga aaagccagtg
     5161 atgatctcaa tgggcaatga aaatgtactg gaaattaagg gaaatgatat tgaccctgaa
     5221 gcagttaaag gtgaagtgtt aaaagttgga aataagagct gtgagaatat acacttacat
     5281 tctgaagccg ttttatgcac ggtccccaat gacctgctga aattgaacag cgagctaaat
     5341 atagagtgga agcaagcaat ttcttcaacc gtccttggaa aagtaatagt tcaaccagat
     5401 cagaatttca caggattgat tgctggtgtt gtctcaatat caacagcact gttattacta
     5461 cttgggtttt tcctgtggct gaaaaagaga aagcaaatta aagatctggg cagtgaatta
     5521 gttcgctacg atgcaagagt acacactcct catttggata ggcttgtaag tgcccgaagt
     5581 gtaagcccaa ctacagaaat ggtttcaaat gaatctgtag actaccgagc tacttttcca
     5641 gaagatcagt ttcctaattc atctcagaac ggttcatgcc gacaagtgca gtatcctctg
     5701 acagacatgt cccccatcct aactagtggg gactctgata tatccagtcc attactgcaa
     5761 aatactgtcc acattgacct cagtgctcta aatccagagc tggtccaggc agtgcagcat
     5821 gtagtgattg ggcccagtag cctgattgtg catttcaatg aagtcatagg aagagggcat
     5881 tttggttgtg tatatcatgg gactttgttg gacaatgatg gcaagaaaat tcactgtgct
     5941 gtgaaatcct tgaacagaat cactgacata ggagaagttt cccaatttct gaccgaggga
     6001 atcatcatga aagattttag tcatcccaat gtcctctcgc tcctgggaat ctgcctgcga
     6061 agtgaagggt ctccgctggt ggtcctacca tacatgaaac atggagatct tcgaaatttc
     6121 attcgaaatg agactcataa tccaactgta aaagatctta ttggctttgg tcttcaagta
     6181 gccaaaggca tgaaatatct tgcaagcaaa aagtttgtcc acagagactt ggctgcaaga
     6241 aactgtatgc tggatgaaaa attcacagtc aaggttgctg attttggtct tgccagagac
     6301 atgtatgata aagaatacta tagtgtacac aacaaaacag gtgcaaagct gccagtgaag
     6361 tggatggctt tggaaagtct gcaaactcaa aagtttacca ccaagtcaga tgtgtggtcc
     6421 tttggcgtgc tcctctggga gctgatgaca agaggagccc caccttatcc tgacgtaaac
     6481 acctttgata taactgttta cttgttgcaa gggagaagac tcctacaacc cgaatactgc
     6541 ccagacccct tatatgaagt aatgctaaaa tgctggcacc ctaaagccga aatgcgccca
     6601 tccttttctg aactggtgtc ccggatatca gcgatcttct ctactttcat tggggagcac
     6661 tatgtccatg tgaacgctac ttatgtgaac gtaaaatgtg tcgctccgta tccttctctg
     6721 ttgtcatcag aagataacgc tgatgatgag gtggacacac gaccagcctc cttctgggag
     6781 acatcagcgg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     6841 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     6901 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     6961 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     7021 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     7081 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     7141 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     7201 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     7261 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     7321 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     7381 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     7441 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     7501 ctcggcatgg acgagctgta caagtaaagc ggccgcgtcg acaatcaacc tctggattac
     7561 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7621 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7681 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7741 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7801 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7861 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7921 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     7981 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8041 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8101 agtcggatct ccctttgggc cgcctccccg cctggaattc taccgggtag gggaggcgct
     8161 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     8221 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     8281 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     8341 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     8401 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     8461 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggnnn
     8521 nnnnnggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     8581 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     8641 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     8701 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     8761 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     8821 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     8881 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     8941 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     9001 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     9061 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     9121 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     9181 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     9241 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     9301 agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc
     9361 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc
     9421 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9601 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     9661 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     9721 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9781 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     9841 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
    10021 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
    10081 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
    10141 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
    10201 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    10261 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    10321 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    10381 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    10441 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    10501 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    10561 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    10621 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
    10681 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    10741 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    10801 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    10861 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    10921 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    10981 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    11041 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    11101 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    11161 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    11221 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    11281 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    11341 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    11401 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    11461 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    11521 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    11581 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    11641 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    11701 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    11761 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    11821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    11881 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    11941 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    12001 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    12061 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    12121 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    12181 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    12241 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    12301 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    12361 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    12421 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    12481 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    12541 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    12601 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    12661 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    12721 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    12781 gagcggataa caatttcaca
//
