LOCUS       Exported                 856 bp ds-DNA     linear   SYN 27-MAY-2021
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    DCT.VV.Orf242
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 27, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             365..406
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             416..433
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(456..473)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    462..686
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(819..838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc cagctgacca ccatggtaat aattccaggt
       61 gtccgttgtt taagtctctt atttctaagg cgtcgatgcc cgctccatat aatatctgcg
      121 tttacgttac tagcaataaa tgcattaata ttgggacata ccatttcgcc agtagatctc
      181 tcatttacta tttgtggata cgagataaga tctatatttg attctgagac agacacgatt
      241 gtcaaattta acgacatcat gtcacagtag gcaattcccg gggatccgaa taaagggtca
      301 agacaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
      361 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca
      421 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
      481 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
      541 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
      601 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
      661 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
      721 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      781 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      841 ctttctcgcc acgttc
//
